Chitosan/Albumin Coating Factorial Optimization of Alginate/Dextran Sulfate Cores for Oral Delivery of Insulin
The design of nanoparticle formulations composed of biopolymers, that govern the physicochemical properties of orally delivered insulin, relies on improving insulin stability and absorption through the intestinal mucosa while protecting it from harsh conditions in the gastrointestinal (GI) tract. Ch...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/3/179 |
| _version_ | 1797610541041057792 |
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| author | Bruno Pessoa Mar Collado-Gonzalez Giuseppina Sandri António Ribeiro |
| author_facet | Bruno Pessoa Mar Collado-Gonzalez Giuseppina Sandri António Ribeiro |
| author_sort | Bruno Pessoa |
| collection | DOAJ |
| description | The design of nanoparticle formulations composed of biopolymers, that govern the physicochemical properties of orally delivered insulin, relies on improving insulin stability and absorption through the intestinal mucosa while protecting it from harsh conditions in the gastrointestinal (GI) tract. Chitosan/polyethylene glycol (PEG) and albumin coating of alginate/dextran sulfate hydrogel cores are presented as a multilayer complex protecting insulin within the nanoparticle. This study aims to optimize a nanoparticle formulation by assessing the relationship between design parameters and experimental data using response surface methodology through a 3-factor 3-level optimization Box–Behnken design. While the selected independent variables were the concentrations of PEG, chitosan and albumin, the dependent variables were particle size, polydispersity index (PDI), zeta potential, and insulin release. Experimental results showed a nanoparticle size ranging from 313 to 585 nm, with PDI from 0.17 to 0.39 and zeta potential ranging from −29 to −44 mV. Insulin bioactivity was maintained in simulated GI media with over 45% cumulative release after 180 min in a simulated intestinal medium. Based on the experimental responses and according to the criteria of desirability on the experimental region’s constraints, solutions of 0.03% PEG, 0.047% chitosan and 1.20% albumin provide an optimum nanoparticle formulation for insulin oral delivery. |
| first_indexed | 2024-03-11T06:16:42Z |
| format | Article |
| id | doaj.art-d79259e5fea6483d80a7ff6ceb431ce3 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T06:16:42Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-d79259e5fea6483d80a7ff6ceb431ce32023-11-17T12:17:39ZengMDPI AGMarine Drugs1660-33972023-03-0121317910.3390/md21030179Chitosan/Albumin Coating Factorial Optimization of Alginate/Dextran Sulfate Cores for Oral Delivery of InsulinBruno Pessoa0Mar Collado-Gonzalez1Giuseppina Sandri2António Ribeiro3Faculty of Pharmacy, University of Coimbra, 3000-048 Coimbra, PortugalFaculty of Pharmacy, University of Coimbra, 3000-048 Coimbra, PortugalDepartment of Drug Sciences, University of Pavia, Viale Taramelli, 12, 27100 Pavia, ItalyFaculty of Pharmacy, University of Coimbra, 3000-048 Coimbra, PortugalThe design of nanoparticle formulations composed of biopolymers, that govern the physicochemical properties of orally delivered insulin, relies on improving insulin stability and absorption through the intestinal mucosa while protecting it from harsh conditions in the gastrointestinal (GI) tract. Chitosan/polyethylene glycol (PEG) and albumin coating of alginate/dextran sulfate hydrogel cores are presented as a multilayer complex protecting insulin within the nanoparticle. This study aims to optimize a nanoparticle formulation by assessing the relationship between design parameters and experimental data using response surface methodology through a 3-factor 3-level optimization Box–Behnken design. While the selected independent variables were the concentrations of PEG, chitosan and albumin, the dependent variables were particle size, polydispersity index (PDI), zeta potential, and insulin release. Experimental results showed a nanoparticle size ranging from 313 to 585 nm, with PDI from 0.17 to 0.39 and zeta potential ranging from −29 to −44 mV. Insulin bioactivity was maintained in simulated GI media with over 45% cumulative release after 180 min in a simulated intestinal medium. Based on the experimental responses and according to the criteria of desirability on the experimental region’s constraints, solutions of 0.03% PEG, 0.047% chitosan and 1.20% albumin provide an optimum nanoparticle formulation for insulin oral delivery.https://www.mdpi.com/1660-3397/21/3/179biopolymersBox–Behnkenfactorial optimizationinsulin deliveryionotropic gelationnanoparticles |
| spellingShingle | Bruno Pessoa Mar Collado-Gonzalez Giuseppina Sandri António Ribeiro Chitosan/Albumin Coating Factorial Optimization of Alginate/Dextran Sulfate Cores for Oral Delivery of Insulin Marine Drugs biopolymers Box–Behnken factorial optimization insulin delivery ionotropic gelation nanoparticles |
| title | Chitosan/Albumin Coating Factorial Optimization of Alginate/Dextran Sulfate Cores for Oral Delivery of Insulin |
| title_full | Chitosan/Albumin Coating Factorial Optimization of Alginate/Dextran Sulfate Cores for Oral Delivery of Insulin |
| title_fullStr | Chitosan/Albumin Coating Factorial Optimization of Alginate/Dextran Sulfate Cores for Oral Delivery of Insulin |
| title_full_unstemmed | Chitosan/Albumin Coating Factorial Optimization of Alginate/Dextran Sulfate Cores for Oral Delivery of Insulin |
| title_short | Chitosan/Albumin Coating Factorial Optimization of Alginate/Dextran Sulfate Cores for Oral Delivery of Insulin |
| title_sort | chitosan albumin coating factorial optimization of alginate dextran sulfate cores for oral delivery of insulin |
| topic | biopolymers Box–Behnken factorial optimization insulin delivery ionotropic gelation nanoparticles |
| url | https://www.mdpi.com/1660-3397/21/3/179 |
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