Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome
Dental stem cells are heterogeneous in their properties. Despite their common origin from neural crest stem cells, they have different functional capacities and biological functions due to niche influence. In this study, we assessed the differences between dental pulp stem cells (DPSC) and periodont...
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MDPI AG
2021-11-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/11/1606 |
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| author | Anastasia V. Kotova Arseniy A. Lobov Julia A. Dombrovskaya Valentina Y. Sannikova Nadezhda A. Ryumina Polina Klausen Alexey L. Shavarda Anna B. Malashicheva Natella I. Enukashvily |
| author_facet | Anastasia V. Kotova Arseniy A. Lobov Julia A. Dombrovskaya Valentina Y. Sannikova Nadezhda A. Ryumina Polina Klausen Alexey L. Shavarda Anna B. Malashicheva Natella I. Enukashvily |
| author_sort | Anastasia V. Kotova |
| collection | DOAJ |
| description | Dental stem cells are heterogeneous in their properties. Despite their common origin from neural crest stem cells, they have different functional capacities and biological functions due to niche influence. In this study, we assessed the differences between dental pulp stem cells (DPSC) and periodontal ligament stem cells (PDLSC) in their pluripotency and neuroepithelial markers transcription, morphological and functional features, osteoblast/odontoblast differentiation and proteomic profile during osteogenic differentiation. The data were collected in paired observations: two cell cultures, DPSC and PDLSC, were obtained from each donor. Both populations had the mesenchymal stem cells surface marker set exposed on their membranes but differed in <i>Nestin</i> (a marker of neuroectodermal origin) expression, morphology, and proliferation rate. <i>OCT4</i> mRNA was revealed in DPSC and PDLSC, while OCT4 protein was present in the nuclei of DPSC only. However, transcription of <i>OCT4</i> mRNA was 1000–10,000-fold lower in dental stem cells than in blastocysts. DPSC proliferated at a slower rate and have a shape closer to polygonal but they responded better to osteogenic stimuli as compared to PDLSC. <i>RUNX2</i> mRNA was detected by qPCR in both types of dental stem cells but RUNX2 protein was detected by LC-MS/MS shotgun proteomics only in PDLSC suggesting the posttranscriptional regulation. <i>DSPP</i> and <i>DMP1</i>, marker genes of odontoblastic type of osteogenic differentiation, were transcribed in DPSC but not in PDLSC samples. Our results prove that DPSC and PDLSC are different in their biology and therapeutic potential: DPSC are a good candidate for osteogenic or odontogenic bone-replacement cell-seeded medicines, while fast proliferating PDLSC are a prospective candidate for other cell products. |
| first_indexed | 2024-03-10T05:41:41Z |
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| id | doaj.art-d7926e8bbff2428596e5e73c3fb71c43 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T05:41:41Z |
| publishDate | 2021-11-01 |
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| series | Biomedicines |
| spelling | doaj.art-d7926e8bbff2428596e5e73c3fb71c432023-11-22T22:30:43ZengMDPI AGBiomedicines2227-90592021-11-01911160610.3390/biomedicines9111606Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and ProteomeAnastasia V. Kotova0Arseniy A. Lobov1Julia A. Dombrovskaya2Valentina Y. Sannikova3Nadezhda A. Ryumina4Polina Klausen5Alexey L. Shavarda6Anna B. Malashicheva7Natella I. Enukashvily8Institute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, RussiaInstitute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, RussiaCell Technologies Laboratory, General Dentistry Department, North-Western State Medical University, 191015 St. Petersburg, RussiaInstitute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, RussiaCell Technologies Center Pokrovsky, LLC, 199106 St. Petersburg, RussiaInstitute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, RussiaResearch Resource Center Molecular and Cell Technologies, Saint-Petersburg State University, 199034 St. Petersburg, RussiaInstitute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, RussiaInstitute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, RussiaDental stem cells are heterogeneous in their properties. Despite their common origin from neural crest stem cells, they have different functional capacities and biological functions due to niche influence. In this study, we assessed the differences between dental pulp stem cells (DPSC) and periodontal ligament stem cells (PDLSC) in their pluripotency and neuroepithelial markers transcription, morphological and functional features, osteoblast/odontoblast differentiation and proteomic profile during osteogenic differentiation. The data were collected in paired observations: two cell cultures, DPSC and PDLSC, were obtained from each donor. Both populations had the mesenchymal stem cells surface marker set exposed on their membranes but differed in <i>Nestin</i> (a marker of neuroectodermal origin) expression, morphology, and proliferation rate. <i>OCT4</i> mRNA was revealed in DPSC and PDLSC, while OCT4 protein was present in the nuclei of DPSC only. However, transcription of <i>OCT4</i> mRNA was 1000–10,000-fold lower in dental stem cells than in blastocysts. DPSC proliferated at a slower rate and have a shape closer to polygonal but they responded better to osteogenic stimuli as compared to PDLSC. <i>RUNX2</i> mRNA was detected by qPCR in both types of dental stem cells but RUNX2 protein was detected by LC-MS/MS shotgun proteomics only in PDLSC suggesting the posttranscriptional regulation. <i>DSPP</i> and <i>DMP1</i>, marker genes of odontoblastic type of osteogenic differentiation, were transcribed in DPSC but not in PDLSC samples. Our results prove that DPSC and PDLSC are different in their biology and therapeutic potential: DPSC are a good candidate for osteogenic or odontogenic bone-replacement cell-seeded medicines, while fast proliferating PDLSC are a prospective candidate for other cell products.https://www.mdpi.com/2227-9059/9/11/1606dental stem cellsdental pulp stem cellsperiodontal ligament stem cellsosteogenic differentiationodontoblastic differentiationpluripotency |
| spellingShingle | Anastasia V. Kotova Arseniy A. Lobov Julia A. Dombrovskaya Valentina Y. Sannikova Nadezhda A. Ryumina Polina Klausen Alexey L. Shavarda Anna B. Malashicheva Natella I. Enukashvily Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome Biomedicines dental stem cells dental pulp stem cells periodontal ligament stem cells osteogenic differentiation odontoblastic differentiation pluripotency |
| title | Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome |
| title_full | Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome |
| title_fullStr | Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome |
| title_full_unstemmed | Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome |
| title_short | Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome |
| title_sort | comparative analysis of dental pulp and periodontal stem cells differences in morphology functionality osteogenic differentiation and proteome |
| topic | dental stem cells dental pulp stem cells periodontal ligament stem cells osteogenic differentiation odontoblastic differentiation pluripotency |
| url | https://www.mdpi.com/2227-9059/9/11/1606 |
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