UVSSA, UBP12, and RDO2/TFIIS Contribute to Arabidopsis UV Tolerance
Plant DNA is damaged by exposure to solar radiation, which includes ultraviolet (UV) rays. UV damaged DNA is repaired either by photolyases, using visible light energy, or by nucleotide excision repair (NER), also known as dark repair. NER consists of two subpathways: global genomic repair (GGR), wh...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00516/full |
| _version_ | 1818448672361283584 |
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| author | Wesam M. Al Khateeb Annan A. Sher Jeffery M. Marcus Dana F. Schroeder |
| author_facet | Wesam M. Al Khateeb Annan A. Sher Jeffery M. Marcus Dana F. Schroeder |
| author_sort | Wesam M. Al Khateeb |
| collection | DOAJ |
| description | Plant DNA is damaged by exposure to solar radiation, which includes ultraviolet (UV) rays. UV damaged DNA is repaired either by photolyases, using visible light energy, or by nucleotide excision repair (NER), also known as dark repair. NER consists of two subpathways: global genomic repair (GGR), which repairs untranscribed DNA throughout the genome, and transcription-coupled repair (TCR), which repairs transcribed DNA. In mammals, CSA, CSB, UVSSA, USP7, and TFIIS have been implicated in TCR. Arabidopsis homologs of CSA (AtCSA-1/2) and CSB (CHR8) have previously been shown to contribute to UV tolerance. Here we examine the role of Arabidopsis homologs of UVSSA, USP7 (UBP12/13), and TFIIS (RDO2) in UV tolerance. We find that loss of function alleles of UVSSA, UBP12, and RDO2 exhibit increased UV sensitivity in both seedlings and adults. UV sensitivity in atcsa-1, uvssa, and ubp12 mutants is specific to dark conditions, consistent with a role in NER. Interestingly, chr8 mutants exhibit UV sensitivity in both light and dark conditions, suggesting that the Arabidopsis CSB homolog may play a role in both NER and light repair. Overall our results indicate a conserved role for UVSSA, USP7 (UBP12), and TFIIS (RDO2) in TCR. |
| first_indexed | 2024-12-14T20:23:14Z |
| format | Article |
| id | doaj.art-d7972309b4d64bd1ba8f13a1842d0b82 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-14T20:23:14Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d7972309b4d64bd1ba8f13a1842d0b822022-12-21T22:48:41ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-04-011010.3389/fpls.2019.00516456624UVSSA, UBP12, and RDO2/TFIIS Contribute to Arabidopsis UV ToleranceWesam M. Al Khateeb0Annan A. Sher1Jeffery M. Marcus2Dana F. Schroeder3Department of Biological Sciences, Yarmouk University, Irbid, JordanDepartment of Biological Sciences, University of Manitoba, Winnipeg, MB, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, MB, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, MB, CanadaPlant DNA is damaged by exposure to solar radiation, which includes ultraviolet (UV) rays. UV damaged DNA is repaired either by photolyases, using visible light energy, or by nucleotide excision repair (NER), also known as dark repair. NER consists of two subpathways: global genomic repair (GGR), which repairs untranscribed DNA throughout the genome, and transcription-coupled repair (TCR), which repairs transcribed DNA. In mammals, CSA, CSB, UVSSA, USP7, and TFIIS have been implicated in TCR. Arabidopsis homologs of CSA (AtCSA-1/2) and CSB (CHR8) have previously been shown to contribute to UV tolerance. Here we examine the role of Arabidopsis homologs of UVSSA, USP7 (UBP12/13), and TFIIS (RDO2) in UV tolerance. We find that loss of function alleles of UVSSA, UBP12, and RDO2 exhibit increased UV sensitivity in both seedlings and adults. UV sensitivity in atcsa-1, uvssa, and ubp12 mutants is specific to dark conditions, consistent with a role in NER. Interestingly, chr8 mutants exhibit UV sensitivity in both light and dark conditions, suggesting that the Arabidopsis CSB homolog may play a role in both NER and light repair. Overall our results indicate a conserved role for UVSSA, USP7 (UBP12), and TFIIS (RDO2) in TCR.https://www.frontiersin.org/article/10.3389/fpls.2019.00516/fullArabidopsistranscription coupled repairUVCSACSBUVSSA |
| spellingShingle | Wesam M. Al Khateeb Annan A. Sher Jeffery M. Marcus Dana F. Schroeder UVSSA, UBP12, and RDO2/TFIIS Contribute to Arabidopsis UV Tolerance Frontiers in Plant Science Arabidopsis transcription coupled repair UV CSA CSB UVSSA |
| title | UVSSA, UBP12, and RDO2/TFIIS Contribute to Arabidopsis UV Tolerance |
| title_full | UVSSA, UBP12, and RDO2/TFIIS Contribute to Arabidopsis UV Tolerance |
| title_fullStr | UVSSA, UBP12, and RDO2/TFIIS Contribute to Arabidopsis UV Tolerance |
| title_full_unstemmed | UVSSA, UBP12, and RDO2/TFIIS Contribute to Arabidopsis UV Tolerance |
| title_short | UVSSA, UBP12, and RDO2/TFIIS Contribute to Arabidopsis UV Tolerance |
| title_sort | uvssa ubp12 and rdo2 tfiis contribute to arabidopsis uv tolerance |
| topic | Arabidopsis transcription coupled repair UV CSA CSB UVSSA |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00516/full |
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