Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial injury in rats
Abstract Background Myocardial injury is considered as a worldwide main cause of morbidity and mortality. The present study aimed to investigate the probable cardioprotective activity of the naturally occurring endogenous fatty acid ester methyl palmitate (MP) against isoproterenol (ISO)-induced myo...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-07-01
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| Series: | Future Journal of Pharmaceutical Sciences |
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| Online Access: | http://link.springer.com/article/10.1186/s43094-020-00044-y |
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| author | Ahmed B. Hamed Eman M. Mantawy Wesam M. El-Bakly Yousra Abdel-Mottaleb Samar S. Azab |
| author_facet | Ahmed B. Hamed Eman M. Mantawy Wesam M. El-Bakly Yousra Abdel-Mottaleb Samar S. Azab |
| author_sort | Ahmed B. Hamed |
| collection | DOAJ |
| description | Abstract Background Myocardial injury is considered as a worldwide main cause of morbidity and mortality. The present study aimed to investigate the probable cardioprotective activity of the naturally occurring endogenous fatty acid ester methyl palmitate (MP) against isoproterenol (ISO)-induced myocardial injury in rats and the possible underlying molecular mechanisms. The study was carried out in two consecutive sets of experiments; the first set screened the cardioprotective dose of MP in ISO-intoxicated rats. In the second set, forty male Sprague Dawley rats received either MP (150 mg/kg, p.o) three times/week for 2 weeks and/or 2 consecutive doses of ISO separated by 24 h (85 mg/kg, s.c) on the 13th and 14th days. Different cardiotoxicity and oxidative stress markers were assessed. Furthermore, endothelial nitric oxide synthase (eNOS) levels were determined. For detection of apoptosis, Bax, Bcl-2, and caspase 3 were estimated. To assess inflammation, toll-like receptor 4 (TLR-4) and tumor necrosis factor-alpha (TNF-α) were measured using ELISA. Meanwhile, nuclear factor kappa B (NF-kB) and cyclooxygenase-2 (COX-2) were detected immunohistochemically. Results Pretreatment with MP significantly ameliorated the cardiotoxicity and oxidative stress markers. It also markedly elevated eNOS content, decreased apoptotic marker expression, and mitigated TLR-4 activation and other inflammatory markers. Electrocardiography and histopathological examination also confirmed the cardioprotective effect of MP. Conclusion The findings of this study indicated that MP possesses a potent cardioprotective activity against ISO-induced myocardial injury through its significant antioxidant, anti-apoptotic, anti-inflammatory, and vasodilatation activities. Graphical abstract |
| first_indexed | 2024-12-11T09:14:03Z |
| format | Article |
| id | doaj.art-d7974ad7f28845aaaca241067d973c68 |
| institution | Directory Open Access Journal |
| issn | 2314-7253 |
| language | English |
| last_indexed | 2024-12-11T09:14:03Z |
| publishDate | 2020-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Future Journal of Pharmaceutical Sciences |
| spelling | doaj.art-d7974ad7f28845aaaca241067d973c682022-12-22T01:13:25ZengSpringerOpenFuture Journal of Pharmaceutical Sciences2314-72532020-07-016111410.1186/s43094-020-00044-yPutative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial injury in ratsAhmed B. Hamed0Eman M. Mantawy1Wesam M. El-Bakly2Yousra Abdel-Mottaleb3Samar S. Azab4Pharmacology, Toxicology and Biochemistry Department, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in EgyptPharmacology and Toxicology Department, Faculty of Pharmacy, Ain shams UniversityPharmacology and Therapeutics Department, Faculty of Medicine, Ain Shams UniversityPharmacology, Toxicology and Biochemistry Department, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in EgyptPharmacology and Toxicology Department, Faculty of Pharmacy, Ain shams UniversityAbstract Background Myocardial injury is considered as a worldwide main cause of morbidity and mortality. The present study aimed to investigate the probable cardioprotective activity of the naturally occurring endogenous fatty acid ester methyl palmitate (MP) against isoproterenol (ISO)-induced myocardial injury in rats and the possible underlying molecular mechanisms. The study was carried out in two consecutive sets of experiments; the first set screened the cardioprotective dose of MP in ISO-intoxicated rats. In the second set, forty male Sprague Dawley rats received either MP (150 mg/kg, p.o) three times/week for 2 weeks and/or 2 consecutive doses of ISO separated by 24 h (85 mg/kg, s.c) on the 13th and 14th days. Different cardiotoxicity and oxidative stress markers were assessed. Furthermore, endothelial nitric oxide synthase (eNOS) levels were determined. For detection of apoptosis, Bax, Bcl-2, and caspase 3 were estimated. To assess inflammation, toll-like receptor 4 (TLR-4) and tumor necrosis factor-alpha (TNF-α) were measured using ELISA. Meanwhile, nuclear factor kappa B (NF-kB) and cyclooxygenase-2 (COX-2) were detected immunohistochemically. Results Pretreatment with MP significantly ameliorated the cardiotoxicity and oxidative stress markers. It also markedly elevated eNOS content, decreased apoptotic marker expression, and mitigated TLR-4 activation and other inflammatory markers. Electrocardiography and histopathological examination also confirmed the cardioprotective effect of MP. Conclusion The findings of this study indicated that MP possesses a potent cardioprotective activity against ISO-induced myocardial injury through its significant antioxidant, anti-apoptotic, anti-inflammatory, and vasodilatation activities. Graphical abstracthttp://link.springer.com/article/10.1186/s43094-020-00044-yMyocardial injuryMethyl palmitateISOOxidative stresseNOSApoptosis |
| spellingShingle | Ahmed B. Hamed Eman M. Mantawy Wesam M. El-Bakly Yousra Abdel-Mottaleb Samar S. Azab Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial injury in rats Future Journal of Pharmaceutical Sciences Myocardial injury Methyl palmitate ISO Oxidative stress eNOS Apoptosis |
| title | Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial injury in rats |
| title_full | Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial injury in rats |
| title_fullStr | Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial injury in rats |
| title_full_unstemmed | Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial injury in rats |
| title_short | Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial injury in rats |
| title_sort | putative anti inflammatory antioxidant and anti apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol induced myocardial injury in rats |
| topic | Myocardial injury Methyl palmitate ISO Oxidative stress eNOS Apoptosis |
| url | http://link.springer.com/article/10.1186/s43094-020-00044-y |
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