Experimental diabetes exacerbates skin transplant rejection in rats
PURPOSE: To investigate the effect of chronic experimental diabetes on skin allografts in rats as a simple model that could clarify some basic aspects and mechanisms involved in transplant rejection in diabetes compared to normal animals. METHODS: Skin grafting was performed with fragments of tail s...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2013-05-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500001&tlng=en |
| _version_ | 1819125776504586240 |
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| author | Flávio Pola dos Reis Angelo Sementilli Antonio Ricardo de Toledo Gagliardi |
| author_facet | Flávio Pola dos Reis Angelo Sementilli Antonio Ricardo de Toledo Gagliardi |
| author_sort | Flávio Pola dos Reis |
| collection | DOAJ |
| description | PURPOSE: To investigate the effect of chronic experimental diabetes on skin allografts in rats as a simple model that could clarify some basic aspects and mechanisms involved in transplant rejection in diabetes compared to normal animals. METHODS: Skin grafting was performed with fragments of tail skin from sex matched non diabetic Wistar rats engrafted onto the thoracic area of diabetic and non diabetic recipients. Grafts were scored for rejection every other day and were removed on day 14. Skin grafts were graded according to the following itens: no rejection; or rejection including: acute, chronic and humoral and/or cellular rejection. Statistical analysis was performed using JMP 5.1 software with ANOVA test. Diabetes was induced with IV injection of alloxan 40 mg/kg. RESULTS: Inflammatory vascular infiltrate compromising the endothelium with areas of fibrinoid necrosis and thrombosis characteristics of acute humoral rejection and subendothelial lymphocyte infiltrate typical of acute cellular rejection were significantly (p<0.003) higher in diabetic than in non diabetic recipients as the inflammatory infiltrate in the epidermis (p<0.002). CONCLUSION: Skin transplant acute rejection from chronic alloxan diabetic rats to normal tissue was significantly more intense than the acute rejection between normal rats. |
| first_indexed | 2024-12-22T07:45:31Z |
| format | Article |
| id | doaj.art-d79b71feb21f4aa489314c96d2cbb8de |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-22T07:45:31Z |
| publishDate | 2013-05-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-d79b71feb21f4aa489314c96d2cbb8de2022-12-21T18:33:39ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502013-05-0128532332610.1590/S0102-86502013000500001Experimental diabetes exacerbates skin transplant rejection in ratsFlávio Pola dos ReisAngelo Sementilli0Antonio Ricardo de Toledo Gagliardi1Universidade Metropolitana de SantosCentro Universitário LusíadaPURPOSE: To investigate the effect of chronic experimental diabetes on skin allografts in rats as a simple model that could clarify some basic aspects and mechanisms involved in transplant rejection in diabetes compared to normal animals. METHODS: Skin grafting was performed with fragments of tail skin from sex matched non diabetic Wistar rats engrafted onto the thoracic area of diabetic and non diabetic recipients. Grafts were scored for rejection every other day and were removed on day 14. Skin grafts were graded according to the following itens: no rejection; or rejection including: acute, chronic and humoral and/or cellular rejection. Statistical analysis was performed using JMP 5.1 software with ANOVA test. Diabetes was induced with IV injection of alloxan 40 mg/kg. RESULTS: Inflammatory vascular infiltrate compromising the endothelium with areas of fibrinoid necrosis and thrombosis characteristics of acute humoral rejection and subendothelial lymphocyte infiltrate typical of acute cellular rejection were significantly (p<0.003) higher in diabetic than in non diabetic recipients as the inflammatory infiltrate in the epidermis (p<0.002). CONCLUSION: Skin transplant acute rejection from chronic alloxan diabetic rats to normal tissue was significantly more intense than the acute rejection between normal rats.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500001&tlng=enTransplantationSkinDiabetes Mellitus, ExperimentalGraft RejectionRats, Wistar |
| spellingShingle | Flávio Pola dos Reis Angelo Sementilli Antonio Ricardo de Toledo Gagliardi Experimental diabetes exacerbates skin transplant rejection in rats Acta Cirúrgica Brasileira Transplantation Skin Diabetes Mellitus, Experimental Graft Rejection Rats, Wistar |
| title | Experimental diabetes exacerbates skin transplant rejection in rats |
| title_full | Experimental diabetes exacerbates skin transplant rejection in rats |
| title_fullStr | Experimental diabetes exacerbates skin transplant rejection in rats |
| title_full_unstemmed | Experimental diabetes exacerbates skin transplant rejection in rats |
| title_short | Experimental diabetes exacerbates skin transplant rejection in rats |
| title_sort | experimental diabetes exacerbates skin transplant rejection in rats |
| topic | Transplantation Skin Diabetes Mellitus, Experimental Graft Rejection Rats, Wistar |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500001&tlng=en |
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