Effect of Cecropin–melittin Chimeric Peptide (CM11) on Trophozoite of Giardia lamblia In Vitro
Background and purpose: Antimicrobial peptides (AMP) are one of the most diverse antimicrobial compounds that have received much attention due to the development of drug resistance of pathogens to conventional antibiotics. But, few studies have evaluated anti-parasitic properties of AMP. The present...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2019-10-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
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| Online Access: | http://jmums.mazums.ac.ir/article-1-13447-en.html |
| _version_ | 1797943956614414336 |
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| author | Somayeh Karimi Tanha Majid Pirestani Javid Sadraei |
| author_facet | Somayeh Karimi Tanha Majid Pirestani Javid Sadraei |
| author_sort | Somayeh Karimi Tanha |
| collection | DOAJ |
| description | Background and purpose: Antimicrobial peptides (AMP) are one of the most diverse antimicrobial compounds that have received much attention due to the development of drug resistance of pathogens to conventional antibiotics. But, few studies have evaluated anti-parasitic properties of AMP. The present study was conducted to compare the effect of a cecropin–melittin chimeric peptide (CM11) and metronidazole on Giardia lamblia trophozoite.
Materials and methods: In this study, using MTT assay, the cytotoxicity of CM-11 peptide (0.5-32 μg/ml) and metronidazole (0.25-25 μg/ml) was investigated on Caco-2 cells and IC50 was calculated. Flow cytometry was used to determine the rate of induction of apoptosis by CM11 and metronidazole. The toxicity of peptide on G. lamblia trophozitis was evaluated in vitro under different conditions.
Results: MTT results showed that the highest percentage of cytotoxicity in caco2 cells was seen at 32 μg/ml of CM11 peptide after 24 (84.3±1.2%), 48 (88.7±0.9%) and 72 (87±1.5%) hours. In metronidazole group, the highest cytotoxicity was observed in 20 μg/ml after 24 (35±2.3), 48 (39.3±1.2) and 72 (48.3±1.8%) hours. The highest concentrations of peptide (32 μg/ml) and metronidazole (20 μg/ml) showed 99.8±0.2% and 99±0.6% cytotoxicity effect, respectively. The cytotoxicity effect of peptide and metronidazole on G. lamblia attached to caco2 cells at highest concentrations were 99.7±0.1% and 99.5±0.3%, respectively.
Conclusion: This study showed that cecropin-melittin chimeric peptide could be an appropriate candidate for the treatment of giardiasis. |
| first_indexed | 2024-04-10T20:32:14Z |
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| id | doaj.art-d7a0fc89ec264042b2c893ed87f6d567 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:32:14Z |
| publishDate | 2019-10-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-d7a0fc89ec264042b2c893ed87f6d5672023-01-25T06:08:39ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792019-10-01291774255Effect of Cecropin–melittin Chimeric Peptide (CM11) on Trophozoite of Giardia lamblia In VitroSomayeh Karimi Tanha0Majid Pirestani1Javid Sadraei2 MSc in Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Assistant Professor, Department of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Associate Professor, Department of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Background and purpose: Antimicrobial peptides (AMP) are one of the most diverse antimicrobial compounds that have received much attention due to the development of drug resistance of pathogens to conventional antibiotics. But, few studies have evaluated anti-parasitic properties of AMP. The present study was conducted to compare the effect of a cecropin–melittin chimeric peptide (CM11) and metronidazole on Giardia lamblia trophozoite. Materials and methods: In this study, using MTT assay, the cytotoxicity of CM-11 peptide (0.5-32 μg/ml) and metronidazole (0.25-25 μg/ml) was investigated on Caco-2 cells and IC50 was calculated. Flow cytometry was used to determine the rate of induction of apoptosis by CM11 and metronidazole. The toxicity of peptide on G. lamblia trophozitis was evaluated in vitro under different conditions. Results: MTT results showed that the highest percentage of cytotoxicity in caco2 cells was seen at 32 μg/ml of CM11 peptide after 24 (84.3±1.2%), 48 (88.7±0.9%) and 72 (87±1.5%) hours. In metronidazole group, the highest cytotoxicity was observed in 20 μg/ml after 24 (35±2.3), 48 (39.3±1.2) and 72 (48.3±1.8%) hours. The highest concentrations of peptide (32 μg/ml) and metronidazole (20 μg/ml) showed 99.8±0.2% and 99±0.6% cytotoxicity effect, respectively. The cytotoxicity effect of peptide and metronidazole on G. lamblia attached to caco2 cells at highest concentrations were 99.7±0.1% and 99.5±0.3%, respectively. Conclusion: This study showed that cecropin-melittin chimeric peptide could be an appropriate candidate for the treatment of giardiasis.http://jmums.mazums.ac.ir/article-1-13447-en.htmlgiardia lambliachimeric peptidececropin-melittinmetronidazolecaco-2 cell |
| spellingShingle | Somayeh Karimi Tanha Majid Pirestani Javid Sadraei Effect of Cecropin–melittin Chimeric Peptide (CM11) on Trophozoite of Giardia lamblia In Vitro Journal of Mazandaran University of Medical Sciences giardia lamblia chimeric peptide cecropin-melittin metronidazole caco-2 cell |
| title | Effect of Cecropin–melittin Chimeric Peptide (CM11) on Trophozoite of Giardia lamblia In Vitro |
| title_full | Effect of Cecropin–melittin Chimeric Peptide (CM11) on Trophozoite of Giardia lamblia In Vitro |
| title_fullStr | Effect of Cecropin–melittin Chimeric Peptide (CM11) on Trophozoite of Giardia lamblia In Vitro |
| title_full_unstemmed | Effect of Cecropin–melittin Chimeric Peptide (CM11) on Trophozoite of Giardia lamblia In Vitro |
| title_short | Effect of Cecropin–melittin Chimeric Peptide (CM11) on Trophozoite of Giardia lamblia In Vitro |
| title_sort | effect of cecropin melittin chimeric peptide cm11 on trophozoite of giardia lamblia in vitro |
| topic | giardia lamblia chimeric peptide cecropin-melittin metronidazole caco-2 cell |
| url | http://jmums.mazums.ac.ir/article-1-13447-en.html |
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