An optimized protocol to detect ubiquitination modification of exogenous or endogenous proteins

Summary: Ubiquitination modification is an important post-translational modification that regulates the stability and function of proteins. Here, we present a protocol to detect the K27-linked polyubiquitination of exogenous and endogenous mitochondrial antiviral signaling protein. We describe steps...

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Detalles Bibliográficos
Main Authors: Xinyu Li, Wei Jiang, Min Fang
Formato: Artigo
Idioma:English
Publicado: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Acceso en liña:http://www.sciencedirect.com/science/article/pii/S2666166723006172