Screening for production of amylase and protease by locally-isolated Bacillus spp. from soil collected in Taguig City and Clark Freeport Zone, Philippines
Amylase and protease are two of the most commonly used enzymes in the pharmaceutical industry. Particularly, Bacillus spp. is widely regarded as a “factory” of these enzymes. In this study, a total of 40 isolates were collected from four soil samples from different metropolitan sites namely, near Ch...
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Mahidol University
2022-03-01
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| Series: | Pharmaceutical Sciences Asia |
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| Online Access: | https://pharmacy.mahidol.ac.th/journal/_files/2022-49-2_11.pdf |
| _version_ | 1819122141781557248 |
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| author | John Paul Matthew Guzman Sheila Mantaring Elizabeth Panerio |
| author_facet | John Paul Matthew Guzman Sheila Mantaring Elizabeth Panerio |
| author_sort | John Paul Matthew Guzman |
| collection | DOAJ |
| description | Amylase and protease are two of the most commonly used enzymes in the pharmaceutical industry. Particularly, Bacillus spp. is widely regarded as a “factory” of these enzymes. In this study, a total of 40 isolates were collected from four soil samples from different metropolitan sites namely, near Chemicals and Energy Division (CED), and National Metrology Laboratory (NML) oil tankers parking lot inside the Department of Science and Technology (DOST) Compound in Taguig City, and two metropolitan-volcanic sites in Clark Freeport Zone, Pampanga (CRK1, CRK2). Of these, 33 isolates were deemed putative Bacillus spp. via phenotypic assays and were screened for the production of amylase and protease. Results showed that 26 of the screened isolates were able to produce protease and 12 were positive for amylase production. Molecular identification revealed that the enzyme-producing isolates were Bacillus spp., B. cereus, B. aryabhattai, B. amyloliquefaciens, B. firmus, B. velezensis, and Fictibacillus sp. No isolate was able to produce amylase alone. These results show the potential of Bacillus spp. from metropolitan soil as sources of pharmaceutically-important enzymes. |
| first_indexed | 2024-12-22T06:47:45Z |
| format | Article |
| id | doaj.art-d7a54173edbf4819947e2e86dd340a67 |
| institution | Directory Open Access Journal |
| issn | 2586-8470 |
| language | English |
| last_indexed | 2024-12-22T06:47:45Z |
| publishDate | 2022-03-01 |
| publisher | Faculty of Pharmacy, Mahidol University |
| record_format | Article |
| series | Pharmaceutical Sciences Asia |
| spelling | doaj.art-d7a54173edbf4819947e2e86dd340a672022-12-21T18:35:15ZengFaculty of Pharmacy, Mahidol UniversityPharmaceutical Sciences Asia2586-84702022-03-0149220220910.29090/psa.2022.02.21.156Screening for production of amylase and protease by locally-isolated Bacillus spp. from soil collected in Taguig City and Clark Freeport Zone, PhilippinesJohn Paul Matthew Guzman0Sheila Mantaring1 Elizabeth Panerio2Industrial Technology Development Institute, Department of Science and Technology, Bicutan, Taguig City, Philippines; The Graduate School, University of Santo Tomas, Espana, Manila, PhilippinesIndustrial Technology Development Institute, Department of Science and Technology, Bicutan, Taguig City, PhilippinesIndustrial Technology Development Institute, Department of Science and Technology, Bicutan, Taguig City, PhilippinesAmylase and protease are two of the most commonly used enzymes in the pharmaceutical industry. Particularly, Bacillus spp. is widely regarded as a “factory” of these enzymes. In this study, a total of 40 isolates were collected from four soil samples from different metropolitan sites namely, near Chemicals and Energy Division (CED), and National Metrology Laboratory (NML) oil tankers parking lot inside the Department of Science and Technology (DOST) Compound in Taguig City, and two metropolitan-volcanic sites in Clark Freeport Zone, Pampanga (CRK1, CRK2). Of these, 33 isolates were deemed putative Bacillus spp. via phenotypic assays and were screened for the production of amylase and protease. Results showed that 26 of the screened isolates were able to produce protease and 12 were positive for amylase production. Molecular identification revealed that the enzyme-producing isolates were Bacillus spp., B. cereus, B. aryabhattai, B. amyloliquefaciens, B. firmus, B. velezensis, and Fictibacillus sp. No isolate was able to produce amylase alone. These results show the potential of Bacillus spp. from metropolitan soil as sources of pharmaceutically-important enzymes.https://pharmacy.mahidol.ac.th/journal/_files/2022-49-2_11.pdfamylasebacillus spp.enzyme productionmetropolitan soilproteasevolcanic soil |
| spellingShingle | John Paul Matthew Guzman Sheila Mantaring Elizabeth Panerio Screening for production of amylase and protease by locally-isolated Bacillus spp. from soil collected in Taguig City and Clark Freeport Zone, Philippines Pharmaceutical Sciences Asia amylase bacillus spp. enzyme production metropolitan soil protease volcanic soil |
| title | Screening for production of amylase and protease by locally-isolated Bacillus spp. from soil collected in Taguig City and Clark Freeport Zone, Philippines |
| title_full | Screening for production of amylase and protease by locally-isolated Bacillus spp. from soil collected in Taguig City and Clark Freeport Zone, Philippines |
| title_fullStr | Screening for production of amylase and protease by locally-isolated Bacillus spp. from soil collected in Taguig City and Clark Freeport Zone, Philippines |
| title_full_unstemmed | Screening for production of amylase and protease by locally-isolated Bacillus spp. from soil collected in Taguig City and Clark Freeport Zone, Philippines |
| title_short | Screening for production of amylase and protease by locally-isolated Bacillus spp. from soil collected in Taguig City and Clark Freeport Zone, Philippines |
| title_sort | screening for production of amylase and protease by locally isolated bacillus spp from soil collected in taguig city and clark freeport zone philippines |
| topic | amylase bacillus spp. enzyme production metropolitan soil protease volcanic soil |
| url | https://pharmacy.mahidol.ac.th/journal/_files/2022-49-2_11.pdf |
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