Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues
Pyrimethanil is one of anilinopyrimidine fungicides widely used in crop protection and frequently found in a variety of crops, including fruits, vegetables, and cereals. This paper describes the development of the first reported immunochromatography test strip (ICTS) based on colloidal gold-labeled...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2020.1733496 |
| Summary: | Pyrimethanil is one of anilinopyrimidine fungicides widely used in crop protection and frequently found in a variety of crops, including fruits, vegetables, and cereals. This paper describes the development of the first reported immunochromatography test strip (ICTS) based on colloidal gold-labeled monoclonal antibody for the detection of pyrimethanil. Under optimal conditions, the visual cut-off value of ICTS for pyrimethanil was 40 ng/mL. Using a portable strip reader, the 50% inhibition concentration and calculated LOD of pyrimethanil in the PBS samples were 4.8 and 0.9 ng/mL, respectively. When used to analyse fortified samples of fruit and vegetable, acceptable recovery rates of 83.3–105.7% with the coefficient of variation values below 11.7% were obtained. Analysis of blindly fortified samples by the proposed ICTS gave similar results to those of the ic-ELISA and HPLC-MS/MS methods. These results indicate the proposed ICTS is a rapid, simple analytical method for quantitative on-site monitoring of pyrimethanil in foodstuffs. |
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| ISSN: | 0954-0105 1465-3443 |