Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues
Pyrimethanil is one of anilinopyrimidine fungicides widely used in crop protection and frequently found in a variety of crops, including fruits, vegetables, and cereals. This paper describes the development of the first reported immunochromatography test strip (ICTS) based on colloidal gold-labeled...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2020.1733496 |
| _version_ | 1818665513769762816 |
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| author | Yong Xie Qiming Kou Qi Sun Yarong Wang Yi Cao Tao Le |
| author_facet | Yong Xie Qiming Kou Qi Sun Yarong Wang Yi Cao Tao Le |
| author_sort | Yong Xie |
| collection | DOAJ |
| description | Pyrimethanil is one of anilinopyrimidine fungicides widely used in crop protection and frequently found in a variety of crops, including fruits, vegetables, and cereals. This paper describes the development of the first reported immunochromatography test strip (ICTS) based on colloidal gold-labeled monoclonal antibody for the detection of pyrimethanil. Under optimal conditions, the visual cut-off value of ICTS for pyrimethanil was 40 ng/mL. Using a portable strip reader, the 50% inhibition concentration and calculated LOD of pyrimethanil in the PBS samples were 4.8 and 0.9 ng/mL, respectively. When used to analyse fortified samples of fruit and vegetable, acceptable recovery rates of 83.3–105.7% with the coefficient of variation values below 11.7% were obtained. Analysis of blindly fortified samples by the proposed ICTS gave similar results to those of the ic-ELISA and HPLC-MS/MS methods. These results indicate the proposed ICTS is a rapid, simple analytical method for quantitative on-site monitoring of pyrimethanil in foodstuffs. |
| first_indexed | 2024-12-17T05:49:50Z |
| format | Article |
| id | doaj.art-d7a9a6a93ad74a77af4f90feb90fde33 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-17T05:49:50Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-d7a9a6a93ad74a77af4f90feb90fde332022-12-21T22:01:12ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432020-01-0131139340510.1080/09540105.2020.17334961733496Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residuesYong Xie0Qiming Kou1Qi Sun2Yarong Wang3Yi Cao4Tao Le5Department of Environmental Art and Design, Chongqing College of Electronic EngineeringCollege of Life Science, Chongqing Normal UniversityCollege of Life Science, Chongqing Normal UniversityCollege of Life Science, Chongqing Normal UniversityDepartment of Environmental Art and Design, Chongqing College of Electronic EngineeringCollege of Life Science, Chongqing Normal UniversityPyrimethanil is one of anilinopyrimidine fungicides widely used in crop protection and frequently found in a variety of crops, including fruits, vegetables, and cereals. This paper describes the development of the first reported immunochromatography test strip (ICTS) based on colloidal gold-labeled monoclonal antibody for the detection of pyrimethanil. Under optimal conditions, the visual cut-off value of ICTS for pyrimethanil was 40 ng/mL. Using a portable strip reader, the 50% inhibition concentration and calculated LOD of pyrimethanil in the PBS samples were 4.8 and 0.9 ng/mL, respectively. When used to analyse fortified samples of fruit and vegetable, acceptable recovery rates of 83.3–105.7% with the coefficient of variation values below 11.7% were obtained. Analysis of blindly fortified samples by the proposed ICTS gave similar results to those of the ic-ELISA and HPLC-MS/MS methods. These results indicate the proposed ICTS is a rapid, simple analytical method for quantitative on-site monitoring of pyrimethanil in foodstuffs.http://dx.doi.org/10.1080/09540105.2020.1733496pyrimethanilimmunochromatography test stripcolloidal goldfungicide residuesfood analysis |
| spellingShingle | Yong Xie Qiming Kou Qi Sun Yarong Wang Yi Cao Tao Le Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues Food and Agricultural Immunology pyrimethanil immunochromatography test strip colloidal gold fungicide residues food analysis |
| title | Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues |
| title_full | Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues |
| title_fullStr | Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues |
| title_full_unstemmed | Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues |
| title_short | Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues |
| title_sort | development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues |
| topic | pyrimethanil immunochromatography test strip colloidal gold fungicide residues food analysis |
| url | http://dx.doi.org/10.1080/09540105.2020.1733496 |
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