Cloning and Sequencing of Leishmania major Thiol-Specific-Antioxidant Antigen (TSA) Gene
Background: Leishmaniasis is caused by parasitic protozoa of the genus Leishmania which, in the infected host are obligate intracellular parasite. TSA is the immuno-dominant antigen of Leishmania major which is considered as the most promising molecule for a recombinant or DNA vaccine against leish...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2007-08-01
|
| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/4244.pdf&manuscript_id=4244 |
| _version_ | 1818897862865453056 |
|---|---|
| author | F Tabatabaie Z Sharifi A Dalimi F Ghaffari far A Zavaran Hoseini |
| author_facet | F Tabatabaie Z Sharifi A Dalimi F Ghaffari far A Zavaran Hoseini |
| author_sort | F Tabatabaie |
| collection | DOAJ |
| description | Background: Leishmaniasis is caused by parasitic protozoa of the genus Leishmania which, in the infected host are obligate intracellular parasite. TSA is the immuno-dominant antigen of Leishmania major which is considered as the most promising molecule for a recombinant or DNA vaccine against leishmaniasis. Methods: Genomic DNA of TSA protein was extracted and amplificated as a template. Then the PCR product was cloned into pTZ57R/T vector. Finally, the recombinant plasmid was extracted from transformed Escherichia coli (TG1 strain) and sequenced. Results: MRHO/IR/75/ER (an Iranian strain) of L. major and TSA gene (Accession number LmjF15.1080) were used. Sequence analysis of cloned TSA gene into pTZ57R/T vector showed high homology of 90% with LmjF15.1080 (TSA gene) and strain "LV39" (Accession no. AF069386) and strain "Friedlin" (Accession no.AF044679). Conclusion: We cloned TSA gene of L. major successfully. Recombinant plasmid was confirmed. It is ready to express recombinant protein for further studies. |
| first_indexed | 2024-12-19T19:22:55Z |
| format | Article |
| id | doaj.art-d7b137bcfb3644fa9cdbd1d3947db670 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-19T19:22:55Z |
| publishDate | 2007-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-d7b137bcfb3644fa9cdbd1d3947db6702022-12-21T20:08:56ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2007-08-01243041Cloning and Sequencing of Leishmania major Thiol-Specific-Antioxidant Antigen (TSA) GeneF TabatabaieZ SharifiA DalimiF Ghaffari farA Zavaran HoseiniBackground: Leishmaniasis is caused by parasitic protozoa of the genus Leishmania which, in the infected host are obligate intracellular parasite. TSA is the immuno-dominant antigen of Leishmania major which is considered as the most promising molecule for a recombinant or DNA vaccine against leishmaniasis. Methods: Genomic DNA of TSA protein was extracted and amplificated as a template. Then the PCR product was cloned into pTZ57R/T vector. Finally, the recombinant plasmid was extracted from transformed Escherichia coli (TG1 strain) and sequenced. Results: MRHO/IR/75/ER (an Iranian strain) of L. major and TSA gene (Accession number LmjF15.1080) were used. Sequence analysis of cloned TSA gene into pTZ57R/T vector showed high homology of 90% with LmjF15.1080 (TSA gene) and strain "LV39" (Accession no. AF069386) and strain "Friedlin" (Accession no.AF044679). Conclusion: We cloned TSA gene of L. major successfully. Recombinant plasmid was confirmed. It is ready to express recombinant protein for further studies.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/4244.pdf&manuscript_id=4244 CloningSequencingLeishmania majorTSA |
| spellingShingle | F Tabatabaie Z Sharifi A Dalimi F Ghaffari far A Zavaran Hoseini Cloning and Sequencing of Leishmania major Thiol-Specific-Antioxidant Antigen (TSA) Gene Iranian Journal of Parasitology Cloning Sequencing Leishmania major TSA |
| title | Cloning and Sequencing of Leishmania major Thiol-Specific-Antioxidant Antigen (TSA) Gene |
| title_full | Cloning and Sequencing of Leishmania major Thiol-Specific-Antioxidant Antigen (TSA) Gene |
| title_fullStr | Cloning and Sequencing of Leishmania major Thiol-Specific-Antioxidant Antigen (TSA) Gene |
| title_full_unstemmed | Cloning and Sequencing of Leishmania major Thiol-Specific-Antioxidant Antigen (TSA) Gene |
| title_short | Cloning and Sequencing of Leishmania major Thiol-Specific-Antioxidant Antigen (TSA) Gene |
| title_sort | cloning and sequencing of leishmania major thiol specific antioxidant antigen tsa gene |
| topic | Cloning Sequencing Leishmania major TSA |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/4244.pdf&manuscript_id=4244 |
| work_keys_str_mv | AT ftabatabaie cloningandsequencingofleishmaniamajorthiolspecificantioxidantantigentsagene AT zsharifi cloningandsequencingofleishmaniamajorthiolspecificantioxidantantigentsagene AT adalimi cloningandsequencingofleishmaniamajorthiolspecificantioxidantantigentsagene AT fghaffarifar cloningandsequencingofleishmaniamajorthiolspecificantioxidantantigentsagene AT azavaranhoseini cloningandsequencingofleishmaniamajorthiolspecificantioxidantantigentsagene |