Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts
Abstract This study investigated the role of vector acquisition and transmission on the propagation of single and co-infections of tomato yellow leaf curl virus (TYLCV,) and tomato mottle virus (ToMoV) (Family: Geminiviridae, Genus: Begomovirus) by the whitefly vector Bemisia tabaci MEAM1 (Gennadius...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-24880-5 |
| _version_ | 1811211130630045696 |
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| author | Autumn A. McLaughlin Linda Hanley-Bowdoin George G. Kennedy Alana L. Jacobson |
| author_facet | Autumn A. McLaughlin Linda Hanley-Bowdoin George G. Kennedy Alana L. Jacobson |
| author_sort | Autumn A. McLaughlin |
| collection | DOAJ |
| description | Abstract This study investigated the role of vector acquisition and transmission on the propagation of single and co-infections of tomato yellow leaf curl virus (TYLCV,) and tomato mottle virus (ToMoV) (Family: Geminiviridae, Genus: Begomovirus) by the whitefly vector Bemisia tabaci MEAM1 (Gennadius) in tomato. The aim of this research was to determine if the manner in which viruses are co-acquired and co-transmitted changes the probability of acquisition, transmission and new host infections. Whiteflies acquired virus by feeding on singly infected plants, co-infected plants, or by sequential feeding on singly infected plants. Viral titers were also quantified by qPCR in vector cohorts, in artificial diet, and plants after exposure to viruliferous vectors. Differences in transmission, infection status of plants, and titers of TYLCV and ToMoV were observed among treatments. All vector cohorts acquired both viruses, but co-acquisition/co-inoculation generally reduced transmission of both viruses as single and mixed infections. Co-inoculation of viruses by the vector also altered virus accumulation in plants regardless of whether one or both viruses were propagated in new hosts. These findings highlight the complex nature of vector-virus-plant interactions that influence the spread and replication of viruses as single and co-infections. |
| first_indexed | 2024-04-12T05:07:32Z |
| format | Article |
| id | doaj.art-d7b1a5ac978745dfa9930fcc24d1014c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T05:07:32Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d7b1a5ac978745dfa9930fcc24d1014c2022-12-22T03:46:50ZengNature PortfolioScientific Reports2045-23222022-11-0112111310.1038/s41598-022-24880-5Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hostsAutumn A. McLaughlin0Linda Hanley-Bowdoin1George G. Kennedy2Alana L. Jacobson3Department of Entomology and Plant Pathology, Auburn UniversityDepartment of Plant and Microbial Biology, North Carolina State UniversityDepartment of Entomology and Plant Pathology, North Carolina State UniversityDepartment of Entomology and Plant Pathology, Auburn UniversityAbstract This study investigated the role of vector acquisition and transmission on the propagation of single and co-infections of tomato yellow leaf curl virus (TYLCV,) and tomato mottle virus (ToMoV) (Family: Geminiviridae, Genus: Begomovirus) by the whitefly vector Bemisia tabaci MEAM1 (Gennadius) in tomato. The aim of this research was to determine if the manner in which viruses are co-acquired and co-transmitted changes the probability of acquisition, transmission and new host infections. Whiteflies acquired virus by feeding on singly infected plants, co-infected plants, or by sequential feeding on singly infected plants. Viral titers were also quantified by qPCR in vector cohorts, in artificial diet, and plants after exposure to viruliferous vectors. Differences in transmission, infection status of plants, and titers of TYLCV and ToMoV were observed among treatments. All vector cohorts acquired both viruses, but co-acquisition/co-inoculation generally reduced transmission of both viruses as single and mixed infections. Co-inoculation of viruses by the vector also altered virus accumulation in plants regardless of whether one or both viruses were propagated in new hosts. These findings highlight the complex nature of vector-virus-plant interactions that influence the spread and replication of viruses as single and co-infections.https://doi.org/10.1038/s41598-022-24880-5 |
| spellingShingle | Autumn A. McLaughlin Linda Hanley-Bowdoin George G. Kennedy Alana L. Jacobson Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts Scientific Reports |
| title | Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts |
| title_full | Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts |
| title_fullStr | Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts |
| title_full_unstemmed | Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts |
| title_short | Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts |
| title_sort | vector acquisition and co inoculation of two plant viruses influences transmission infection and replication in new hosts |
| url | https://doi.org/10.1038/s41598-022-24880-5 |
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