The Immunomodulatory Aspect of Quercetin Penta Acetate on Th17 Cells Proliferation and Gene Expression in Multiple Sclerosis
ObjectiveThe function of Th17 cells in the neuroinflammatory process in multiple sclerosis has been clarified. The production of IL-17 is dependent on the gene expression of RORc. It has been suggested that Quercetin can influence MS due to a variety of anti-inflammatory effects. Acetylation can enh...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2023-02-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_254753_ed01aacd312dd3ae20e0d4e8d9a85af9.pdf |
| _version_ | 1797894830175551488 |
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| author | Leila Ahmadi Nahid Eskandari Mustafa Ghanadian Mahshid Rahmati Neda Kasiri Masoud Etamadifar Mohadeseh Toghyani Fereshteh Alsahebfosoul |
| author_facet | Leila Ahmadi Nahid Eskandari Mustafa Ghanadian Mahshid Rahmati Neda Kasiri Masoud Etamadifar Mohadeseh Toghyani Fereshteh Alsahebfosoul |
| author_sort | Leila Ahmadi |
| collection | DOAJ |
| description | ObjectiveThe function of Th17 cells in the neuroinflammatory process in multiple sclerosis has been clarified. The production of IL-17 is dependent on the gene expression of RORc. It has been suggested that Quercetin can influence MS due to a variety of anti-inflammatory effects. Acetylation can enhance lipophilia that is an important feature to cross the Blood-Brain-Barrier, so Quercetin Penta Acetate may cause more improvement for patients. The aim of the present study was to examine in vitro immunomodulatory aspect of Quercetin Penta Acetate as a modified compound on Th17 cells of MS patients, also compared with Quercetin and Methyl Prednisolone Acetate.Materials and MethodsIn present experimental study, PBMCs isolated and stained CFSE then, IC50 values were determined using different doses and times for Quercetin Penta Acetate, and Methyl Prednisolone Acetate. Th17 cells proliferation by flow cytometry and gene expression of IL-17 and RORc by real-time PCR method were analyzed.ResultsThe results showed that IL-17A gene expression was inhibited by Quercetin (P = 0.0081), , and Quercetin Penta Acetate did not have a significant inhibitory effect on Th17 cells proliferation (P= 0.59) and RORc gene expression (P =0.1). Our results showed some immunomodulatory aspects of Quercetin Penta Acetate on Th17 cells is more effective than Quercetin (P= 0.018).ConclusionTaken together, it suggests that Quercetin Penta Acetate can be considered in control MS because of its more potent immunomodulatory properties on Th17 cells compare with Quercetin in some aspects and or as a natural based might be listed among other medications in MS. |
| first_indexed | 2024-04-10T07:16:42Z |
| format | Article |
| id | doaj.art-d7b20efe2cfb446db24d210b3c1827c4 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-04-10T07:16:42Z |
| publishDate | 2023-02-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-d7b20efe2cfb446db24d210b3c1827c42023-02-25T09:41:26ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142023-02-0125211011710.22074/cellj.2022.557560.1073254753The Immunomodulatory Aspect of Quercetin Penta Acetate on Th17 Cells Proliferation and Gene Expression in Multiple SclerosisLeila Ahmadi0Nahid Eskandari1Mustafa Ghanadian2Mahshid Rahmati3Neda Kasiri4Masoud Etamadifar5Mohadeseh Toghyani6Fereshteh Alsahebfosoul7Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IranIsfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IranObjectiveThe function of Th17 cells in the neuroinflammatory process in multiple sclerosis has been clarified. The production of IL-17 is dependent on the gene expression of RORc. It has been suggested that Quercetin can influence MS due to a variety of anti-inflammatory effects. Acetylation can enhance lipophilia that is an important feature to cross the Blood-Brain-Barrier, so Quercetin Penta Acetate may cause more improvement for patients. The aim of the present study was to examine in vitro immunomodulatory aspect of Quercetin Penta Acetate as a modified compound on Th17 cells of MS patients, also compared with Quercetin and Methyl Prednisolone Acetate.Materials and MethodsIn present experimental study, PBMCs isolated and stained CFSE then, IC50 values were determined using different doses and times for Quercetin Penta Acetate, and Methyl Prednisolone Acetate. Th17 cells proliferation by flow cytometry and gene expression of IL-17 and RORc by real-time PCR method were analyzed.ResultsThe results showed that IL-17A gene expression was inhibited by Quercetin (P = 0.0081), , and Quercetin Penta Acetate did not have a significant inhibitory effect on Th17 cells proliferation (P= 0.59) and RORc gene expression (P =0.1). Our results showed some immunomodulatory aspects of Quercetin Penta Acetate on Th17 cells is more effective than Quercetin (P= 0.018).ConclusionTaken together, it suggests that Quercetin Penta Acetate can be considered in control MS because of its more potent immunomodulatory properties on Th17 cells compare with Quercetin in some aspects and or as a natural based might be listed among other medications in MS.https://www.celljournal.org/article_254753_ed01aacd312dd3ae20e0d4e8d9a85af9.pdfmultiple sclerosisprednisolonequercetinth17 cells |
| spellingShingle | Leila Ahmadi Nahid Eskandari Mustafa Ghanadian Mahshid Rahmati Neda Kasiri Masoud Etamadifar Mohadeseh Toghyani Fereshteh Alsahebfosoul The Immunomodulatory Aspect of Quercetin Penta Acetate on Th17 Cells Proliferation and Gene Expression in Multiple Sclerosis Cell Journal multiple sclerosis prednisolone quercetin th17 cells |
| title | The Immunomodulatory Aspect of Quercetin Penta Acetate on Th17 Cells Proliferation and Gene Expression in Multiple Sclerosis |
| title_full | The Immunomodulatory Aspect of Quercetin Penta Acetate on Th17 Cells Proliferation and Gene Expression in Multiple Sclerosis |
| title_fullStr | The Immunomodulatory Aspect of Quercetin Penta Acetate on Th17 Cells Proliferation and Gene Expression in Multiple Sclerosis |
| title_full_unstemmed | The Immunomodulatory Aspect of Quercetin Penta Acetate on Th17 Cells Proliferation and Gene Expression in Multiple Sclerosis |
| title_short | The Immunomodulatory Aspect of Quercetin Penta Acetate on Th17 Cells Proliferation and Gene Expression in Multiple Sclerosis |
| title_sort | immunomodulatory aspect of quercetin penta acetate on th17 cells proliferation and gene expression in multiple sclerosis |
| topic | multiple sclerosis prednisolone quercetin th17 cells |
| url | https://www.celljournal.org/article_254753_ed01aacd312dd3ae20e0d4e8d9a85af9.pdf |
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