Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia
Lipoprotein and endothelial lipases are members of the triglyceride lipase gene family. These genes are expressed in the brain, where the encoded proteins are fulfilling functions that have yet to be elucidated. In this study, we examined the distribution of their respective mRNAs in the C57BL/6 mou...
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Elsevier
2004-03-01
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Series: | Neurobiology of Disease |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996103001980 |
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author | É Paradis S Clavel P Julien M.R.V Murthy F de Bilbao D Arsenijevic P Giannakopoulos P Vallet D Richard |
author_facet | É Paradis S Clavel P Julien M.R.V Murthy F de Bilbao D Arsenijevic P Giannakopoulos P Vallet D Richard |
author_sort | É Paradis |
collection | DOAJ |
description | Lipoprotein and endothelial lipases are members of the triglyceride lipase gene family. These genes are expressed in the brain, where the encoded proteins are fulfilling functions that have yet to be elucidated. In this study, we examined the distribution of their respective mRNAs in the C57BL/6 mouse brain by in situ hybridization. In control mice, we observed widespread expression of lipoprotein lipase (LPL) mRNA mainly in pyramidal cells of the hippocampus (CA1, CA2 and CA3 areas), in the striatum and in several cortical areas. Endothelial lipase (EL) mRNA expression was restricted to CA3 pyramidal cells of the hippocampus, to ependymal cells in the ventral part of the third ventricle and to some cortical cell layers. To gain insight into the role played by lipases in the brain, neurodegeneration was induced by intraperitoneal injection of kainic acid (KA) or by occlusion of the middle cerebral artery (MCA). Upon injection of KA, a rapid increase in EL mRNA expression was observed in the piriform cortex, hippocampus, thalamus and neocortex. However, the levels of LPL mRNA were unaffected by KA injection. Remarkably, after focal cerebral ischemia, the expression of EL was unaffected whereas a dramatic increase in LPL expression was observed in neocortical areas of the lesioned side of the brain. These results show that LPL and EL transcripts are selectively upregulated in function of the type of brain injury. LPL and EL could thus fulfill a function in the pathophysiological response of the brain to injury. |
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spelling | doaj.art-d7b53e7d9bc14cefbff4d663a7c864d32022-12-21T18:36:14ZengElsevierNeurobiology of Disease1095-953X2004-03-01152312325Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemiaÉ Paradis0S Clavel1P Julien2M.R.V Murthy3F de Bilbao4D Arsenijevic5P Giannakopoulos6P Vallet7D Richard8Department of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandLipoprotein and endothelial lipases are members of the triglyceride lipase gene family. These genes are expressed in the brain, where the encoded proteins are fulfilling functions that have yet to be elucidated. In this study, we examined the distribution of their respective mRNAs in the C57BL/6 mouse brain by in situ hybridization. In control mice, we observed widespread expression of lipoprotein lipase (LPL) mRNA mainly in pyramidal cells of the hippocampus (CA1, CA2 and CA3 areas), in the striatum and in several cortical areas. Endothelial lipase (EL) mRNA expression was restricted to CA3 pyramidal cells of the hippocampus, to ependymal cells in the ventral part of the third ventricle and to some cortical cell layers. To gain insight into the role played by lipases in the brain, neurodegeneration was induced by intraperitoneal injection of kainic acid (KA) or by occlusion of the middle cerebral artery (MCA). Upon injection of KA, a rapid increase in EL mRNA expression was observed in the piriform cortex, hippocampus, thalamus and neocortex. However, the levels of LPL mRNA were unaffected by KA injection. Remarkably, after focal cerebral ischemia, the expression of EL was unaffected whereas a dramatic increase in LPL expression was observed in neocortical areas of the lesioned side of the brain. These results show that LPL and EL transcripts are selectively upregulated in function of the type of brain injury. LPL and EL could thus fulfill a function in the pathophysiological response of the brain to injury.http://www.sciencedirect.com/science/article/pii/S0969996103001980LipaseIschemiaExcitotoxicityLipidmRNA distributionIn situ hybridization |
spellingShingle | É Paradis S Clavel P Julien M.R.V Murthy F de Bilbao D Arsenijevic P Giannakopoulos P Vallet D Richard Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia Neurobiology of Disease Lipase Ischemia Excitotoxicity Lipid mRNA distribution In situ hybridization |
title | Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia |
title_full | Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia |
title_fullStr | Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia |
title_full_unstemmed | Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia |
title_short | Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia |
title_sort | lipoprotein lipase and endothelial lipase expression in mouse brain regional distribution and selective induction following kainic acid induced lesion and focal cerebral ischemia |
topic | Lipase Ischemia Excitotoxicity Lipid mRNA distribution In situ hybridization |
url | http://www.sciencedirect.com/science/article/pii/S0969996103001980 |
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