Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia

Lipoprotein and endothelial lipases are members of the triglyceride lipase gene family. These genes are expressed in the brain, where the encoded proteins are fulfilling functions that have yet to be elucidated. In this study, we examined the distribution of their respective mRNAs in the C57BL/6 mou...

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Main Authors: É Paradis, S Clavel, P Julien, M.R.V Murthy, F de Bilbao, D Arsenijevic, P Giannakopoulos, P Vallet, D Richard
Format: Article
Language:English
Published: Elsevier 2004-03-01
Series:Neurobiology of Disease
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0969996103001980
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author É Paradis
S Clavel
P Julien
M.R.V Murthy
F de Bilbao
D Arsenijevic
P Giannakopoulos
P Vallet
D Richard
author_facet É Paradis
S Clavel
P Julien
M.R.V Murthy
F de Bilbao
D Arsenijevic
P Giannakopoulos
P Vallet
D Richard
author_sort É Paradis
collection DOAJ
description Lipoprotein and endothelial lipases are members of the triglyceride lipase gene family. These genes are expressed in the brain, where the encoded proteins are fulfilling functions that have yet to be elucidated. In this study, we examined the distribution of their respective mRNAs in the C57BL/6 mouse brain by in situ hybridization. In control mice, we observed widespread expression of lipoprotein lipase (LPL) mRNA mainly in pyramidal cells of the hippocampus (CA1, CA2 and CA3 areas), in the striatum and in several cortical areas. Endothelial lipase (EL) mRNA expression was restricted to CA3 pyramidal cells of the hippocampus, to ependymal cells in the ventral part of the third ventricle and to some cortical cell layers. To gain insight into the role played by lipases in the brain, neurodegeneration was induced by intraperitoneal injection of kainic acid (KA) or by occlusion of the middle cerebral artery (MCA). Upon injection of KA, a rapid increase in EL mRNA expression was observed in the piriform cortex, hippocampus, thalamus and neocortex. However, the levels of LPL mRNA were unaffected by KA injection. Remarkably, after focal cerebral ischemia, the expression of EL was unaffected whereas a dramatic increase in LPL expression was observed in neocortical areas of the lesioned side of the brain. These results show that LPL and EL transcripts are selectively upregulated in function of the type of brain injury. LPL and EL could thus fulfill a function in the pathophysiological response of the brain to injury.
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spelling doaj.art-d7b53e7d9bc14cefbff4d663a7c864d32022-12-21T18:36:14ZengElsevierNeurobiology of Disease1095-953X2004-03-01152312325Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemiaÉ Paradis0S Clavel1P Julien2M.R.V Murthy3F de Bilbao4D Arsenijevic5P Giannakopoulos6P Vallet7D Richard8Department of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandDepartment of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Department of Physiology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada; Laval University Medical Center, Laval University, Ste-Foy (PQ), Canada; Department of Psychiatry, University Hospital of Geneva, Switzerland; Department of Psychogenetics, University Hospitals of Lausanne, Switzerland; Gruppe Physiologie und Tierhaltung ETHZ, Zurich, SwitzerlandLipoprotein and endothelial lipases are members of the triglyceride lipase gene family. These genes are expressed in the brain, where the encoded proteins are fulfilling functions that have yet to be elucidated. In this study, we examined the distribution of their respective mRNAs in the C57BL/6 mouse brain by in situ hybridization. In control mice, we observed widespread expression of lipoprotein lipase (LPL) mRNA mainly in pyramidal cells of the hippocampus (CA1, CA2 and CA3 areas), in the striatum and in several cortical areas. Endothelial lipase (EL) mRNA expression was restricted to CA3 pyramidal cells of the hippocampus, to ependymal cells in the ventral part of the third ventricle and to some cortical cell layers. To gain insight into the role played by lipases in the brain, neurodegeneration was induced by intraperitoneal injection of kainic acid (KA) or by occlusion of the middle cerebral artery (MCA). Upon injection of KA, a rapid increase in EL mRNA expression was observed in the piriform cortex, hippocampus, thalamus and neocortex. However, the levels of LPL mRNA were unaffected by KA injection. Remarkably, after focal cerebral ischemia, the expression of EL was unaffected whereas a dramatic increase in LPL expression was observed in neocortical areas of the lesioned side of the brain. These results show that LPL and EL transcripts are selectively upregulated in function of the type of brain injury. LPL and EL could thus fulfill a function in the pathophysiological response of the brain to injury.http://www.sciencedirect.com/science/article/pii/S0969996103001980LipaseIschemiaExcitotoxicityLipidmRNA distributionIn situ hybridization
spellingShingle É Paradis
S Clavel
P Julien
M.R.V Murthy
F de Bilbao
D Arsenijevic
P Giannakopoulos
P Vallet
D Richard
Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia
Neurobiology of Disease
Lipase
Ischemia
Excitotoxicity
Lipid
mRNA distribution
In situ hybridization
title Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia
title_full Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia
title_fullStr Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia
title_full_unstemmed Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia
title_short Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia
title_sort lipoprotein lipase and endothelial lipase expression in mouse brain regional distribution and selective induction following kainic acid induced lesion and focal cerebral ischemia
topic Lipase
Ischemia
Excitotoxicity
Lipid
mRNA distribution
In situ hybridization
url http://www.sciencedirect.com/science/article/pii/S0969996103001980
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