Lethal impacts of cigarette smoke in cultured tobacco cells
Background In order to understand and generalize the toxic mechanism of cigarette smoke in living cells, comparison of the data between animal systems and other biological system such as microbial and plant systems is highly beneficial. Objective By employing the tobacco cells as model materials fo...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
European Publishing
2011-07-01
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| Series: | Tobacco Induced Diseases |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/tid/Lethal-impacts-of-cigarette-smoke-in-cultured-tobacco-cells,66049,0,2.html |
| _version_ | 1818357778297651200 |
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| author | Masaru Yukihiro Takuya Hiramatsu Tomonori Kawano |
| author_facet | Masaru Yukihiro Takuya Hiramatsu Tomonori Kawano |
| author_sort | Masaru Yukihiro |
| collection | DOAJ |
| description | Background
In order to understand and generalize the toxic mechanism of cigarette smoke in living cells,
comparison of the data between animal systems and other biological system such as microbial and plant systems
is highly beneficial.
Objective
By employing the tobacco cells as model materials for cigarette smoke toxicity assay, the impacts of
the combustion by-products such as nitrogen oxides could be highlighted as the toxic impacts of the plantderived
endogenous chemicals could be excluded in the plant cells.
Methods
Cigarette smoke-induced cell death was assessed in tobacco cell suspension cultures in the presence
and absence of pharmacological inhibitors.
Results
Cigarette smoke was effective in induction of cell death. The smoke-induced cell death could be partially
prevented by addition of nitric oxide (NO) scavenger, suggesting the role for NO as the cell death mediator.
Addition of NO donor to tobacco cells also resulted in development of partial cell death further confirming the
role of NO as cell death mediator. Members of reactive oxygen species and calcium ion were shown to be
protecting the cells from the toxic action of smoke-derived NO. |
| first_indexed | 2024-12-13T20:18:31Z |
| format | Article |
| id | doaj.art-d7b63abecad3431d9b4ee5152a332bb7 |
| institution | Directory Open Access Journal |
| issn | 1617-9625 |
| language | English |
| last_indexed | 2024-12-13T20:18:31Z |
| publishDate | 2011-07-01 |
| publisher | European Publishing |
| record_format | Article |
| series | Tobacco Induced Diseases |
| spelling | doaj.art-d7b63abecad3431d9b4ee5152a332bb72022-12-21T23:32:45ZengEuropean PublishingTobacco Induced Diseases1617-96252011-07-019July10.1186/1617-9625-9-866049Lethal impacts of cigarette smoke in cultured tobacco cellsMasaru Yukihiro0Takuya Hiramatsu1Tomonori Kawano2Laboratory of Chemical Biology and Bioengineering, Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, JapanLaboratory of Chemical Biology and Bioengineering, Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, JapanLaboratory of Chemical Biology and Bioengineering, Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, JapanBackground In order to understand and generalize the toxic mechanism of cigarette smoke in living cells, comparison of the data between animal systems and other biological system such as microbial and plant systems is highly beneficial. Objective By employing the tobacco cells as model materials for cigarette smoke toxicity assay, the impacts of the combustion by-products such as nitrogen oxides could be highlighted as the toxic impacts of the plantderived endogenous chemicals could be excluded in the plant cells. Methods Cigarette smoke-induced cell death was assessed in tobacco cell suspension cultures in the presence and absence of pharmacological inhibitors. Results Cigarette smoke was effective in induction of cell death. The smoke-induced cell death could be partially prevented by addition of nitric oxide (NO) scavenger, suggesting the role for NO as the cell death mediator. Addition of NO donor to tobacco cells also resulted in development of partial cell death further confirming the role of NO as cell death mediator. Members of reactive oxygen species and calcium ion were shown to be protecting the cells from the toxic action of smoke-derived NO.http://www.journalssystem.com/tid/Lethal-impacts-of-cigarette-smoke-in-cultured-tobacco-cells,66049,0,2.htmlreactive oxygen speciesnitric oxidenicotinecigarette smokeprogramme cell death |
| spellingShingle | Masaru Yukihiro Takuya Hiramatsu Tomonori Kawano Lethal impacts of cigarette smoke in cultured tobacco cells Tobacco Induced Diseases reactive oxygen species nitric oxide nicotine cigarette smoke programme cell death |
| title | Lethal impacts of cigarette smoke in cultured tobacco cells |
| title_full | Lethal impacts of cigarette smoke in cultured tobacco cells |
| title_fullStr | Lethal impacts of cigarette smoke in cultured tobacco cells |
| title_full_unstemmed | Lethal impacts of cigarette smoke in cultured tobacco cells |
| title_short | Lethal impacts of cigarette smoke in cultured tobacco cells |
| title_sort | lethal impacts of cigarette smoke in cultured tobacco cells |
| topic | reactive oxygen species nitric oxide nicotine cigarette smoke programme cell death |
| url | http://www.journalssystem.com/tid/Lethal-impacts-of-cigarette-smoke-in-cultured-tobacco-cells,66049,0,2.html |
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