Short-Term Lincomycin Exposure Depletion of Murine Microbiota Affects Short-Chain Fatty Acids and Intestinal Morphology and Immunity
Lincomycin, as one of the most commonly used antibiotics, may cause intestinal injury, enteritis and other side effects, but it remains unknown whether these effects are associated with microbial changes and the effects of different doses of lincomycin on infants. Here, 21-day old mice were exposed...
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MDPI AG
2020-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/12/907 |
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| author | Shunfen Zhang Ruqing Zhong Hui Han Bao Yi Jie Yin Liang Chen Hongfu Zhang |
| author_facet | Shunfen Zhang Ruqing Zhong Hui Han Bao Yi Jie Yin Liang Chen Hongfu Zhang |
| author_sort | Shunfen Zhang |
| collection | DOAJ |
| description | Lincomycin, as one of the most commonly used antibiotics, may cause intestinal injury, enteritis and other side effects, but it remains unknown whether these effects are associated with microbial changes and the effects of different doses of lincomycin on infants. Here, 21-day old mice were exposed to 1 and 5 g/L lincomycin to explore the effects of lincomycin on the gut microbiota, metabolites and inflammation. Compared to the control mice, 1 g/L lincomycin exposure decreased the body weight gain of mice (<i>p</i> < 0.05). Both 1 and 5 g/L lincomycin exposure reduced the diversity and microbial composition of mice (<i>p <</i> 0.05). Furthermore, 1 and 5 g/L lincomycin reduced the relative concentrations of acetate, propionate, butyrate, valerate, isobutyric acid and isovaleric acid in the colon chyme of mice (<i>p <</i> 0.05). In addition, 5 g/L lincomycin exposure reduced the villus height, crypt depth, and relative expression of <i>TLR2</i>, <i>TLR3</i>, <i>TLR4</i>, <i>IL-18</i>, <i>TNF-α</i>, and <i>p65</i> in the jejunum of mice (<i>p</i> < 0.05), while 1 g/L lincomycin exposure reduced the relative expression of <i>TLR2</i>, <i>TLR3</i>, <i>TNF-α</i>, and <i>p65</i> (<i>p <</i> 0.05). Collectively, these results highlight the depletion effect of short-term lincomycin exposure on microbiota and the further regulatory effect on intestinal morphology and immunosuppression in infant mice. |
| first_indexed | 2024-03-10T14:05:20Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T14:05:20Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-d7ba8254ffca4849a34ca1339db141932023-11-21T00:45:19ZengMDPI AGAntibiotics2079-63822020-12-0191290710.3390/antibiotics9120907Short-Term Lincomycin Exposure Depletion of Murine Microbiota Affects Short-Chain Fatty Acids and Intestinal Morphology and ImmunityShunfen Zhang0Ruqing Zhong1Hui Han2Bao Yi3Jie Yin4Liang Chen5Hongfu Zhang6State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaLincomycin, as one of the most commonly used antibiotics, may cause intestinal injury, enteritis and other side effects, but it remains unknown whether these effects are associated with microbial changes and the effects of different doses of lincomycin on infants. Here, 21-day old mice were exposed to 1 and 5 g/L lincomycin to explore the effects of lincomycin on the gut microbiota, metabolites and inflammation. Compared to the control mice, 1 g/L lincomycin exposure decreased the body weight gain of mice (<i>p</i> < 0.05). Both 1 and 5 g/L lincomycin exposure reduced the diversity and microbial composition of mice (<i>p <</i> 0.05). Furthermore, 1 and 5 g/L lincomycin reduced the relative concentrations of acetate, propionate, butyrate, valerate, isobutyric acid and isovaleric acid in the colon chyme of mice (<i>p <</i> 0.05). In addition, 5 g/L lincomycin exposure reduced the villus height, crypt depth, and relative expression of <i>TLR2</i>, <i>TLR3</i>, <i>TLR4</i>, <i>IL-18</i>, <i>TNF-α</i>, and <i>p65</i> in the jejunum of mice (<i>p</i> < 0.05), while 1 g/L lincomycin exposure reduced the relative expression of <i>TLR2</i>, <i>TLR3</i>, <i>TNF-α</i>, and <i>p65</i> (<i>p <</i> 0.05). Collectively, these results highlight the depletion effect of short-term lincomycin exposure on microbiota and the further regulatory effect on intestinal morphology and immunosuppression in infant mice.https://www.mdpi.com/2079-6382/9/12/907antibioticmicrobiotametabolitesTLRintestinal morphology |
| spellingShingle | Shunfen Zhang Ruqing Zhong Hui Han Bao Yi Jie Yin Liang Chen Hongfu Zhang Short-Term Lincomycin Exposure Depletion of Murine Microbiota Affects Short-Chain Fatty Acids and Intestinal Morphology and Immunity Antibiotics antibiotic microbiota metabolites TLR intestinal morphology |
| title | Short-Term Lincomycin Exposure Depletion of Murine Microbiota Affects Short-Chain Fatty Acids and Intestinal Morphology and Immunity |
| title_full | Short-Term Lincomycin Exposure Depletion of Murine Microbiota Affects Short-Chain Fatty Acids and Intestinal Morphology and Immunity |
| title_fullStr | Short-Term Lincomycin Exposure Depletion of Murine Microbiota Affects Short-Chain Fatty Acids and Intestinal Morphology and Immunity |
| title_full_unstemmed | Short-Term Lincomycin Exposure Depletion of Murine Microbiota Affects Short-Chain Fatty Acids and Intestinal Morphology and Immunity |
| title_short | Short-Term Lincomycin Exposure Depletion of Murine Microbiota Affects Short-Chain Fatty Acids and Intestinal Morphology and Immunity |
| title_sort | short term lincomycin exposure depletion of murine microbiota affects short chain fatty acids and intestinal morphology and immunity |
| topic | antibiotic microbiota metabolites TLR intestinal morphology |
| url | https://www.mdpi.com/2079-6382/9/12/907 |
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