Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysis
The photogeneration of nitric oxide (NO) using laser flash photolysis was investigated for S-nitroso-glutathione (GSNO) and S-nitroso-N-acetylcysteine (NacySNO) at pH 6.4 (PBS/HCl) and 7.4 (PBS). Irradiation of S-nitrosothiol with light (lambda = 355 nm followed by absorption spectroscopy) resulted...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2003-05-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500005 |
| _version_ | 1818476957986193408 |
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| author | J.C.G. Rotta C.N. Lunardi A.C. Tedesco |
| author_facet | J.C.G. Rotta C.N. Lunardi A.C. Tedesco |
| author_sort | J.C.G. Rotta |
| collection | DOAJ |
| description | The photogeneration of nitric oxide (NO) using laser flash photolysis was investigated for S-nitroso-glutathione (GSNO) and S-nitroso-N-acetylcysteine (NacySNO) at pH 6.4 (PBS/HCl) and 7.4 (PBS). Irradiation of S-nitrosothiol with light (lambda = 355 nm followed by absorption spectroscopy) resulted in the homolytic decomposition of NacySNO and GSNO to generate radicals (GS· and NacyS·) and NO. The release of NO from donor compounds measured with an ISO-Nometer apparatus was larger at pH 7.4 than pH 6.4. NacySNO was also incorporated into dipalmitoyl-phosphatidylcholine liposomes in the presence and absence of zinc phthalocyanine (ZnPC), a well-known photosensitizer useful for photodynamic therapy. Liposomes are usually used as carriers for hydrophobic compounds such as ZnPC. Inclusion of ZnPC resulted in a decrease in NO liberation in liposomal medium. However, there was a synergistic action of both photosensitizers and S-nitrosothiols resulting in the formation of other reactive species such as peroxynitrite, which is a potent oxidizing agent. These data show that NO release depends on pH and the medium, as well as on the laser energy applied to the system. Changes in the absorption spectrum were monitored as a function of light exposure. |
| first_indexed | 2024-12-10T09:31:59Z |
| format | Article |
| id | doaj.art-d7bac2793aaf4f64a856296eb0cd74bb |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-10T09:31:59Z |
| publishDate | 2003-05-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-d7bac2793aaf4f64a856296eb0cd74bb2022-12-22T01:54:19ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2003-05-0136558759410.1590/S0100-879X2003000500005Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysisJ.C.G. RottaC.N. LunardiA.C. TedescoThe photogeneration of nitric oxide (NO) using laser flash photolysis was investigated for S-nitroso-glutathione (GSNO) and S-nitroso-N-acetylcysteine (NacySNO) at pH 6.4 (PBS/HCl) and 7.4 (PBS). Irradiation of S-nitrosothiol with light (lambda = 355 nm followed by absorption spectroscopy) resulted in the homolytic decomposition of NacySNO and GSNO to generate radicals (GS· and NacyS·) and NO. The release of NO from donor compounds measured with an ISO-Nometer apparatus was larger at pH 7.4 than pH 6.4. NacySNO was also incorporated into dipalmitoyl-phosphatidylcholine liposomes in the presence and absence of zinc phthalocyanine (ZnPC), a well-known photosensitizer useful for photodynamic therapy. Liposomes are usually used as carriers for hydrophobic compounds such as ZnPC. Inclusion of ZnPC resulted in a decrease in NO liberation in liposomal medium. However, there was a synergistic action of both photosensitizers and S-nitrosothiols resulting in the formation of other reactive species such as peroxynitrite, which is a potent oxidizing agent. These data show that NO release depends on pH and the medium, as well as on the laser energy applied to the system. Changes in the absorption spectrum were monitored as a function of light exposure.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500005S-nitrosothiolsNitric oxideZinc phthalocyanineLiposomesPhotodynamic therapy |
| spellingShingle | J.C.G. Rotta C.N. Lunardi A.C. Tedesco Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysis Brazilian Journal of Medical and Biological Research S-nitrosothiols Nitric oxide Zinc phthalocyanine Liposomes Photodynamic therapy |
| title | Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysis |
| title_full | Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysis |
| title_fullStr | Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysis |
| title_full_unstemmed | Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysis |
| title_short | Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysis |
| title_sort | nitric oxide release from the s nitrosothiol zinc phthalocyanine complex by flash photolysis |
| topic | S-nitrosothiols Nitric oxide Zinc phthalocyanine Liposomes Photodynamic therapy |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500005 |
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