Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells
CTCs (circulating tumor cells) are well-known for their use in clinical trials for tumor diagnosis. Capturing and isolating these CTCs from whole blood samples has enormous benefits in cancer diagnosis and treatment. In general, various approaches are being used to separate malignant cells, includin...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/4/220 |
| _version_ | 1797436713917743104 |
|---|---|
| author | Mahesh Padmalaya Bhat Venkatachalam Thendral Uluvangada Thammaiah Uthappa Kyeong-Hwan Lee Madhuprasad Kigga Tariq Altalhi Mahaveer D. Kurkuri Krishna Kant |
| author_facet | Mahesh Padmalaya Bhat Venkatachalam Thendral Uluvangada Thammaiah Uthappa Kyeong-Hwan Lee Madhuprasad Kigga Tariq Altalhi Mahaveer D. Kurkuri Krishna Kant |
| author_sort | Mahesh Padmalaya Bhat |
| collection | DOAJ |
| description | CTCs (circulating tumor cells) are well-known for their use in clinical trials for tumor diagnosis. Capturing and isolating these CTCs from whole blood samples has enormous benefits in cancer diagnosis and treatment. In general, various approaches are being used to separate malignant cells, including immunomagnets, macroscale filters, centrifuges, dielectrophoresis, and immunological approaches. These procedures, on the other hand, are time-consuming and necessitate multiple high-level operational protocols. In addition, considering their low efficiency and throughput, the processes of capturing and isolating CTCs face tremendous challenges. Meanwhile, recent advances in microfluidic devices promise unprecedented advantages for capturing and isolating CTCs with greater efficiency, sensitivity, selectivity and accuracy. In this regard, this review article focuses primarily on the various fabrication methodologies involved in microfluidic devices and techniques specifically used to capture and isolate CTCs using various physical and biological methods as well as their conceptual ideas, advantages and disadvantages. |
| first_indexed | 2024-03-09T11:05:34Z |
| format | Article |
| id | doaj.art-d7bd51070d8744b3bc3e249b5b83646e |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T11:05:34Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-d7bd51070d8744b3bc3e249b5b83646e2023-12-01T00:58:05ZengMDPI AGBiosensors2079-63742022-04-0112422010.3390/bios12040220Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor CellsMahesh Padmalaya Bhat0Venkatachalam Thendral1Uluvangada Thammaiah Uthappa2Kyeong-Hwan Lee3Madhuprasad Kigga4Tariq Altalhi5Mahaveer D. Kurkuri6Krishna Kant7Centre for Research in Functional Materials (CRFM), Jain Global Campus, Jain University, Bengaluru 562112, Karnataka, IndiaCentre for Research in Functional Materials (CRFM), Jain Global Campus, Jain University, Bengaluru 562112, Karnataka, IndiaSchool of Chemical Engineering, Yeungnam University, 280 Daehak-ro, Gyeongsan 38541, KoreaAgricultural Automation Research Center, Chonnam National University, Gwangju 61186, KoreaCentre for Research in Functional Materials (CRFM), Jain Global Campus, Jain University, Bengaluru 562112, Karnataka, IndiaDepartment of Chemistry, Faculty of Science, Taif University, Taif 21944, Saudi ArabiaCentre for Research in Functional Materials (CRFM), Jain Global Campus, Jain University, Bengaluru 562112, Karnataka, IndiaDepartamento de Química Física, Campus Universitario, CINBIO Universidade de Vigo, 36310 Vigo, SpainCTCs (circulating tumor cells) are well-known for their use in clinical trials for tumor diagnosis. Capturing and isolating these CTCs from whole blood samples has enormous benefits in cancer diagnosis and treatment. In general, various approaches are being used to separate malignant cells, including immunomagnets, macroscale filters, centrifuges, dielectrophoresis, and immunological approaches. These procedures, on the other hand, are time-consuming and necessitate multiple high-level operational protocols. In addition, considering their low efficiency and throughput, the processes of capturing and isolating CTCs face tremendous challenges. Meanwhile, recent advances in microfluidic devices promise unprecedented advantages for capturing and isolating CTCs with greater efficiency, sensitivity, selectivity and accuracy. In this regard, this review article focuses primarily on the various fabrication methodologies involved in microfluidic devices and techniques specifically used to capture and isolate CTCs using various physical and biological methods as well as their conceptual ideas, advantages and disadvantages.https://www.mdpi.com/2079-6374/12/4/220circulating tumor cells (CTCs)microfluidic devicephysical methodbiological methodcancer diagnostics |
| spellingShingle | Mahesh Padmalaya Bhat Venkatachalam Thendral Uluvangada Thammaiah Uthappa Kyeong-Hwan Lee Madhuprasad Kigga Tariq Altalhi Mahaveer D. Kurkuri Krishna Kant Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells Biosensors circulating tumor cells (CTCs) microfluidic device physical method biological method cancer diagnostics |
| title | Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells |
| title_full | Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells |
| title_fullStr | Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells |
| title_full_unstemmed | Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells |
| title_short | Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells |
| title_sort | recent advances in microfluidic platform for physical and immunological detection and capture of circulating tumor cells |
| topic | circulating tumor cells (CTCs) microfluidic device physical method biological method cancer diagnostics |
| url | https://www.mdpi.com/2079-6374/12/4/220 |
| work_keys_str_mv | AT maheshpadmalayabhat recentadvancesinmicrofluidicplatformforphysicalandimmunologicaldetectionandcaptureofcirculatingtumorcells AT venkatachalamthendral recentadvancesinmicrofluidicplatformforphysicalandimmunologicaldetectionandcaptureofcirculatingtumorcells AT uluvangadathammaiahuthappa recentadvancesinmicrofluidicplatformforphysicalandimmunologicaldetectionandcaptureofcirculatingtumorcells AT kyeonghwanlee recentadvancesinmicrofluidicplatformforphysicalandimmunologicaldetectionandcaptureofcirculatingtumorcells AT madhuprasadkigga recentadvancesinmicrofluidicplatformforphysicalandimmunologicaldetectionandcaptureofcirculatingtumorcells AT tariqaltalhi recentadvancesinmicrofluidicplatformforphysicalandimmunologicaldetectionandcaptureofcirculatingtumorcells AT mahaveerdkurkuri recentadvancesinmicrofluidicplatformforphysicalandimmunologicaldetectionandcaptureofcirculatingtumorcells AT krishnakant recentadvancesinmicrofluidicplatformforphysicalandimmunologicaldetectionandcaptureofcirculatingtumorcells |