A novel mouse model of ischemic carotid artery disease.
<h4>Background</h4>Carotid artery occlusive disease gradually develops over time, eventually leading to cerebral infarction and high mortality rate. Animal models replicating cerebral infarction resulting from carotid artery occlusive disease have thus been developed to test potential no...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24940742/?tool=EBI |
| _version_ | 1819181000895234048 |
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| author | Yorito Hattori Akihiro Kitamura Kazuyuki Nagatsuka Masafumi Ihara |
| author_facet | Yorito Hattori Akihiro Kitamura Kazuyuki Nagatsuka Masafumi Ihara |
| author_sort | Yorito Hattori |
| collection | DOAJ |
| description | <h4>Background</h4>Carotid artery occlusive disease gradually develops over time, eventually leading to cerebral infarction and high mortality rate. Animal models replicating cerebral infarction resulting from carotid artery occlusive disease have thus been developed to test potential novel treatments, which could be subsequently administered clinically.<h4>Methods</h4>Adult C57BL/6J male mice were subjected to ameroid constrictor (AC) placement to gradually narrow the bilateral common carotid arteries. Cerebral blood flow (CBF) was measured at several time points. At 7 and 28 days post-operation, post-mortem brain samples were analyzed for ischemic changes.<h4>Results</h4>The mortality rate was 58.8% at 28 days post-operation. Surviving mice with AC showed continuous reduction of CBF by up to 70% of the baseline level at 28 days. Most of the mice (75%) showed multiple cerebral infarctions in the gray and white matter. Non-surviving mice showed critical CBF reduction below 20-30% of the baseline level before death.<h4>Conclusion</h4>The application of the AC on the bilateral common carotid arteries in mice could offer a reliable model of severe cerebrovascular insufficiency due to carotid artery occlusive disease and may thus be useful in exploring pharmacological intervention in stroke through monitoring survival rate, infarct formation, and CBF profile. |
| first_indexed | 2024-12-22T22:23:17Z |
| format | Article |
| id | doaj.art-d7c0c1ea29b24b58817aca16f1fc3d4c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T22:23:17Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d7c0c1ea29b24b58817aca16f1fc3d4c2022-12-21T18:10:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e10025710.1371/journal.pone.0100257A novel mouse model of ischemic carotid artery disease.Yorito HattoriAkihiro KitamuraKazuyuki NagatsukaMasafumi Ihara<h4>Background</h4>Carotid artery occlusive disease gradually develops over time, eventually leading to cerebral infarction and high mortality rate. Animal models replicating cerebral infarction resulting from carotid artery occlusive disease have thus been developed to test potential novel treatments, which could be subsequently administered clinically.<h4>Methods</h4>Adult C57BL/6J male mice were subjected to ameroid constrictor (AC) placement to gradually narrow the bilateral common carotid arteries. Cerebral blood flow (CBF) was measured at several time points. At 7 and 28 days post-operation, post-mortem brain samples were analyzed for ischemic changes.<h4>Results</h4>The mortality rate was 58.8% at 28 days post-operation. Surviving mice with AC showed continuous reduction of CBF by up to 70% of the baseline level at 28 days. Most of the mice (75%) showed multiple cerebral infarctions in the gray and white matter. Non-surviving mice showed critical CBF reduction below 20-30% of the baseline level before death.<h4>Conclusion</h4>The application of the AC on the bilateral common carotid arteries in mice could offer a reliable model of severe cerebrovascular insufficiency due to carotid artery occlusive disease and may thus be useful in exploring pharmacological intervention in stroke through monitoring survival rate, infarct formation, and CBF profile.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24940742/?tool=EBI |
| spellingShingle | Yorito Hattori Akihiro Kitamura Kazuyuki Nagatsuka Masafumi Ihara A novel mouse model of ischemic carotid artery disease. PLoS ONE |
| title | A novel mouse model of ischemic carotid artery disease. |
| title_full | A novel mouse model of ischemic carotid artery disease. |
| title_fullStr | A novel mouse model of ischemic carotid artery disease. |
| title_full_unstemmed | A novel mouse model of ischemic carotid artery disease. |
| title_short | A novel mouse model of ischemic carotid artery disease. |
| title_sort | novel mouse model of ischemic carotid artery disease |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24940742/?tool=EBI |
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