Genome-Wide Identification of <i>Phytophthora sojae</i>-Associated microRNAs and Network in a Resistant and a Susceptible Soybean Germplasm
Phytophthora root rot, caused by <i>Phytophthora sojae</i> (<i>P. sojae</i>), is one of the most devastating diseases limiting soybean production worldwide. microRNAs (miRNAs) play major roles in regulating plant defense against pathogens. To understand the roles of soybean m...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/12/2922 |
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| author | Na Guo Ammara Tahir Xiaoxia Cui Jianyu Xu Jutao Sun Nannan Zhang Ruidong Sun Sushuang Deng Han Xing Jinming Zhao |
| author_facet | Na Guo Ammara Tahir Xiaoxia Cui Jianyu Xu Jutao Sun Nannan Zhang Ruidong Sun Sushuang Deng Han Xing Jinming Zhao |
| author_sort | Na Guo |
| collection | DOAJ |
| description | Phytophthora root rot, caused by <i>Phytophthora sojae</i> (<i>P. sojae</i>), is one of the most devastating diseases limiting soybean production worldwide. microRNAs (miRNAs) play major roles in regulating plant defense against pathogens. To understand the roles of soybean miRNAs during <i>P. sojae</i> infection, we analyzed four small RNA libraries from two soybean germplasms before and after <i>P. sojae</i> isolate JS08-12 infection. The cultivar Nannong 10-1 was resistant to JS08-12, whereas the 06-070583 line was susceptible to JS08-12. In total, 528 known and 555 putative novel miRNAs in soybean were identified from 97 million reads; 74 known miRNAs and 75 novel miRNAs that might be specifically related to Nannong10-1 responses to <i>P. sojae</i>; and 55 known and 43 novel miRNAs expressed before and after infection in the susceptible line 06-070583. qRT-PCR provided similar miRNA expression patterns to those obtained by the small-RNA sequencing of the four libraries. Then, the potential target genes of these differentially expressed miRNA were predicted, which encoded transcriptional factors, resistance proteins and transporters. Finally, we focused on the targets of the three legume-specific miRNAs (gma-miR1508, gma-miR1509, and gma-miR1510) and charted the miRNA–target interactions and networks based on the published degradome data. |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T17:26:23Z |
| publishDate | 2022-11-01 |
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| series | Agronomy |
| spelling | doaj.art-d7c2b28e3b344871b65647a11acf8b562023-11-24T12:43:22ZengMDPI AGAgronomy2073-43952022-11-011212292210.3390/agronomy12122922Genome-Wide Identification of <i>Phytophthora sojae</i>-Associated microRNAs and Network in a Resistant and a Susceptible Soybean GermplasmNa Guo0Ammara Tahir1Xiaoxia Cui2Jianyu Xu3Jutao Sun4Nannan Zhang5Ruidong Sun6Sushuang Deng7Han Xing8Jinming Zhao9National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, ChinaPhytophthora root rot, caused by <i>Phytophthora sojae</i> (<i>P. sojae</i>), is one of the most devastating diseases limiting soybean production worldwide. microRNAs (miRNAs) play major roles in regulating plant defense against pathogens. To understand the roles of soybean miRNAs during <i>P. sojae</i> infection, we analyzed four small RNA libraries from two soybean germplasms before and after <i>P. sojae</i> isolate JS08-12 infection. The cultivar Nannong 10-1 was resistant to JS08-12, whereas the 06-070583 line was susceptible to JS08-12. In total, 528 known and 555 putative novel miRNAs in soybean were identified from 97 million reads; 74 known miRNAs and 75 novel miRNAs that might be specifically related to Nannong10-1 responses to <i>P. sojae</i>; and 55 known and 43 novel miRNAs expressed before and after infection in the susceptible line 06-070583. qRT-PCR provided similar miRNA expression patterns to those obtained by the small-RNA sequencing of the four libraries. Then, the potential target genes of these differentially expressed miRNA were predicted, which encoded transcriptional factors, resistance proteins and transporters. Finally, we focused on the targets of the three legume-specific miRNAs (gma-miR1508, gma-miR1509, and gma-miR1510) and charted the miRNA–target interactions and networks based on the published degradome data.https://www.mdpi.com/2073-4395/12/12/2922<i>Glycine max</i><i>Phytophthora sojae</i>microRNARNA sequencingresistance |
| spellingShingle | Na Guo Ammara Tahir Xiaoxia Cui Jianyu Xu Jutao Sun Nannan Zhang Ruidong Sun Sushuang Deng Han Xing Jinming Zhao Genome-Wide Identification of <i>Phytophthora sojae</i>-Associated microRNAs and Network in a Resistant and a Susceptible Soybean Germplasm Agronomy <i>Glycine max</i> <i>Phytophthora sojae</i> microRNA RNA sequencing resistance |
| title | Genome-Wide Identification of <i>Phytophthora sojae</i>-Associated microRNAs and Network in a Resistant and a Susceptible Soybean Germplasm |
| title_full | Genome-Wide Identification of <i>Phytophthora sojae</i>-Associated microRNAs and Network in a Resistant and a Susceptible Soybean Germplasm |
| title_fullStr | Genome-Wide Identification of <i>Phytophthora sojae</i>-Associated microRNAs and Network in a Resistant and a Susceptible Soybean Germplasm |
| title_full_unstemmed | Genome-Wide Identification of <i>Phytophthora sojae</i>-Associated microRNAs and Network in a Resistant and a Susceptible Soybean Germplasm |
| title_short | Genome-Wide Identification of <i>Phytophthora sojae</i>-Associated microRNAs and Network in a Resistant and a Susceptible Soybean Germplasm |
| title_sort | genome wide identification of i phytophthora sojae i associated micrornas and network in a resistant and a susceptible soybean germplasm |
| topic | <i>Glycine max</i> <i>Phytophthora sojae</i> microRNA RNA sequencing resistance |
| url | https://www.mdpi.com/2073-4395/12/12/2922 |
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