Dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitro
Dendritic outgrowth and arborization are important for establishing neural circuit formation. To date, little information exists about the involvement of the extracellular matrix (ECM) and its cellular receptors in these processes. In our studies, we focus on the role of dystroglycan (DG), a cell ad...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-05-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00199/full |
| _version_ | 1818495620155965440 |
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| author | Monika eBijata Jakub eWlodarczyk Izabela eFigiel |
| author_facet | Monika eBijata Jakub eWlodarczyk Izabela eFigiel |
| author_sort | Monika eBijata |
| collection | DOAJ |
| description | Dendritic outgrowth and arborization are important for establishing neural circuit formation. To date, little information exists about the involvement of the extracellular matrix (ECM) and its cellular receptors in these processes. In our studies, we focus on the role of dystroglycan (DG), a cell adhesion molecule that links ECM components to the actin cytoskeleton, in dendritic development and branching. Using a lentiviral vector (LV) to deliver short-hairpin RNA (shRNA) that specifically silences DG in cultured hippocampal neurons, we found that DG knockdown exerted an inhibitory effect on dendritic tree growth and arborization. The structural changes were associated with activation of the guanosine triphosphatase (GTPase) Cdc42. The overexpression of DG promoted dendritic length and branching. Furthermore, exposure of the cultures to autoactivating matrix metalloproteinase-9 (aaMMP-9), a β-DG-cleaving protease, decreased the complexity of dendritic arbors. This effect was abolished in neurons that overexpressed a β-DG mutant that was defective in MMP-9-mediated cleavage. Altogether, our results indicate that DG controls dendritic arborization in vitro in MMP-9-dependent manner. |
| first_indexed | 2024-12-10T18:23:04Z |
| format | Article |
| id | doaj.art-d7c55ea42e8e4bccbb609bc9a21342b8 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-10T18:23:04Z |
| publishDate | 2015-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-d7c55ea42e8e4bccbb609bc9a21342b82022-12-22T01:38:09ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022015-05-01910.3389/fncel.2015.00199141893Dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitroMonika eBijata0Jakub eWlodarczyk1Izabela eFigiel2Nencki Institute of Experimental BiologyNencki Institute of Experimental BiologyNencki Institute of Experimental BiologyDendritic outgrowth and arborization are important for establishing neural circuit formation. To date, little information exists about the involvement of the extracellular matrix (ECM) and its cellular receptors in these processes. In our studies, we focus on the role of dystroglycan (DG), a cell adhesion molecule that links ECM components to the actin cytoskeleton, in dendritic development and branching. Using a lentiviral vector (LV) to deliver short-hairpin RNA (shRNA) that specifically silences DG in cultured hippocampal neurons, we found that DG knockdown exerted an inhibitory effect on dendritic tree growth and arborization. The structural changes were associated with activation of the guanosine triphosphatase (GTPase) Cdc42. The overexpression of DG promoted dendritic length and branching. Furthermore, exposure of the cultures to autoactivating matrix metalloproteinase-9 (aaMMP-9), a β-DG-cleaving protease, decreased the complexity of dendritic arbors. This effect was abolished in neurons that overexpressed a β-DG mutant that was defective in MMP-9-mediated cleavage. Altogether, our results indicate that DG controls dendritic arborization in vitro in MMP-9-dependent manner.http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00199/fullExtracellular MatrixCdc42DystroglycanMMP-9dendritic arborization |
| spellingShingle | Monika eBijata Jakub eWlodarczyk Izabela eFigiel Dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitro Frontiers in Cellular Neuroscience Extracellular Matrix Cdc42 Dystroglycan MMP-9 dendritic arborization |
| title | Dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitro |
| title_full | Dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitro |
| title_fullStr | Dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitro |
| title_full_unstemmed | Dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitro |
| title_short | Dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitro |
| title_sort | dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitro |
| topic | Extracellular Matrix Cdc42 Dystroglycan MMP-9 dendritic arborization |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00199/full |
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