Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma
Abstract Isolation of pure extracellular vesicles (EVs), especially from blood, has been a major challenge in the field of EV research. The presence of lipoproteins and soluble proteins often hinders the isolation of high purity EVs upon utilization of conventional separation methods. To circumvent...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-80514-8 |
| _version_ | 1818716232341258240 |
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| author | Jik-Han Jung Woojin Back Junyong Yoon Hyeonjeong Han Ka-Won Kang Byeonghyeon Choi Hyesun Jeong Jaena Park Hyunku Shin Woojune Hur Yeonho Choi Sunghoi Hong Hyun Koo Kim Yong Park Ji-Ho Park |
| author_facet | Jik-Han Jung Woojin Back Junyong Yoon Hyeonjeong Han Ka-Won Kang Byeonghyeon Choi Hyesun Jeong Jaena Park Hyunku Shin Woojune Hur Yeonho Choi Sunghoi Hong Hyun Koo Kim Yong Park Ji-Ho Park |
| author_sort | Jik-Han Jung |
| collection | DOAJ |
| description | Abstract Isolation of pure extracellular vesicles (EVs), especially from blood, has been a major challenge in the field of EV research. The presence of lipoproteins and soluble proteins often hinders the isolation of high purity EVs upon utilization of conventional separation methods. To circumvent such problems, we designed a single-step dual size-exclusion chromatography (dSEC) column for effective isolation of highly pure EVs from bone marrow derived human plasma. With an aim to select appropriate column design parameters, we analyzed the physiochemical properties of the major substances in bone marrow derived plasma, which include EVs, lipoproteins, and soluble proteins. Based on these findings, we devised a novel dSEC column with two different types of porous beads sequentially stacked each other for efficient separation of EVs from other contaminants. The newly developed dSEC columns exhibited better performance in isolating highly pure EVs from AML plasma in comparison to conventional isolation methods. |
| first_indexed | 2024-12-17T19:15:59Z |
| format | Article |
| id | doaj.art-d7c58063b9d64ae4a0715170544b6155 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T19:15:59Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d7c58063b9d64ae4a0715170544b61552022-12-21T21:35:45ZengNature PortfolioScientific Reports2045-23222021-01-011111910.1038/s41598-020-80514-8Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasmaJik-Han Jung0Woojin Back1Junyong Yoon2Hyeonjeong Han3Ka-Won Kang4Byeonghyeon Choi5Hyesun Jeong6Jaena Park7Hyunku Shin8Woojune Hur9Yeonho Choi10Sunghoi Hong11Hyun Koo Kim12Yong Park13Ji-Ho Park14Department of Bio and Brain Engineering and KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology (KAIST)Department of Bio and Brain Engineering and KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology (KAIST)Department of Bio and Brain Engineering and KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology (KAIST)Department of Bio and Brain Engineering and KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology (KAIST)Division of Hematology-Oncology, Department of Internal Medicine, Korea University College of MedicineDepartment of Thoracic and Cardiovascular Surgery and Department of Biomedical Sciences, Korea University College of MedicineSchool of Biosystems and Biomedical Sciences, Korea UniversityDepartment of Biomedical Engineering, Korea UniversityDepartment of Biomedical Engineering, Korea UniversityDivision of Hematology-Oncology, Department of Internal Medicine, Korea University College of MedicineDepartment of Biomedical Engineering, Korea UniversitySchool of Biosystems and Biomedical Sciences, Korea UniversityDepartment of Thoracic and Cardiovascular Surgery and Department of Biomedical Sciences, Korea University College of MedicineDivision of Hematology-Oncology, Department of Internal Medicine, Korea University College of MedicineDepartment of Bio and Brain Engineering and KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology (KAIST)Abstract Isolation of pure extracellular vesicles (EVs), especially from blood, has been a major challenge in the field of EV research. The presence of lipoproteins and soluble proteins often hinders the isolation of high purity EVs upon utilization of conventional separation methods. To circumvent such problems, we designed a single-step dual size-exclusion chromatography (dSEC) column for effective isolation of highly pure EVs from bone marrow derived human plasma. With an aim to select appropriate column design parameters, we analyzed the physiochemical properties of the major substances in bone marrow derived plasma, which include EVs, lipoproteins, and soluble proteins. Based on these findings, we devised a novel dSEC column with two different types of porous beads sequentially stacked each other for efficient separation of EVs from other contaminants. The newly developed dSEC columns exhibited better performance in isolating highly pure EVs from AML plasma in comparison to conventional isolation methods.https://doi.org/10.1038/s41598-020-80514-8 |
| spellingShingle | Jik-Han Jung Woojin Back Junyong Yoon Hyeonjeong Han Ka-Won Kang Byeonghyeon Choi Hyesun Jeong Jaena Park Hyunku Shin Woojune Hur Yeonho Choi Sunghoi Hong Hyun Koo Kim Yong Park Ji-Ho Park Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma Scientific Reports |
| title | Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma |
| title_full | Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma |
| title_fullStr | Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma |
| title_full_unstemmed | Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma |
| title_short | Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma |
| title_sort | dual size exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma |
| url | https://doi.org/10.1038/s41598-020-80514-8 |
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