Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study
Abstract Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds resolution. Here, a D-FFOCT module design is presented...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-023-05378-w |
| _version_ | 1797557174154559488 |
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| author | Tual Monfort Salvatore Azzollini Jérémy Brogard Marilou Clémençon Amélie Slembrouck-Brec Valerie Forster Serge Picaud Olivier Goureau Sacha Reichman Olivier Thouvenin Kate Grieve |
| author_facet | Tual Monfort Salvatore Azzollini Jérémy Brogard Marilou Clémençon Amélie Slembrouck-Brec Valerie Forster Serge Picaud Olivier Goureau Sacha Reichman Olivier Thouvenin Kate Grieve |
| author_sort | Tual Monfort |
| collection | DOAJ |
| description | Abstract Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds resolution. Here, a D-FFOCT module design is presented which can be coupled to a commercial microscope with a stage top incubator, allowing non-invasive label-free longitudinal imaging over periods of minutes to weeks on the same sample. Long term volumetric imaging on human induced pluripotent stem cell-derived retinal organoids is demonstrated, highlighting tissue and cell organization processes such as rosette formation and mitosis as well as cell shape and motility. Imaging on retinal explants highlights single 3D cone and rod structures. An optimal workflow for data acquisition, postprocessing and saving is demonstrated, resulting in a time gain factor of 10 compared to prior state of the art. Finally, a method to increase D-FFOCT signal-to-noise ratio is demonstrated, allowing rapid organoid screening. |
| first_indexed | 2024-03-10T17:13:36Z |
| format | Article |
| id | doaj.art-d7c6030c66464d5a921793a68612340c |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-03-10T17:13:36Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-d7c6030c66464d5a921793a68612340c2023-11-20T10:36:29ZengNature PortfolioCommunications Biology2399-36422023-09-016111710.1038/s42003-023-05378-wDynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture studyTual Monfort0Salvatore Azzollini1Jérémy Brogard2Marilou Clémençon3Amélie Slembrouck-Brec4Valerie Forster5Serge Picaud6Olivier Goureau7Sacha Reichman8Olivier Thouvenin9Kate Grieve10Sorbonne Université, INSERM, CNRS, Institut de la VisionSorbonne Université, INSERM, CNRS, Institut de la VisionSorbonne Université, INSERM, CNRS, Institut de la VisionSorbonne Université, INSERM, CNRS, Institut de la VisionSorbonne Université, INSERM, CNRS, Institut de la VisionSorbonne Université, INSERM, CNRS, Institut de la VisionSorbonne Université, INSERM, CNRS, Institut de la VisionSorbonne Université, INSERM, CNRS, Institut de la VisionSorbonne Université, INSERM, CNRS, Institut de la VisionInstitut Langevin, ESPCI Paris, Université PSL, CNRSSorbonne Université, INSERM, CNRS, Institut de la VisionAbstract Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds resolution. Here, a D-FFOCT module design is presented which can be coupled to a commercial microscope with a stage top incubator, allowing non-invasive label-free longitudinal imaging over periods of minutes to weeks on the same sample. Long term volumetric imaging on human induced pluripotent stem cell-derived retinal organoids is demonstrated, highlighting tissue and cell organization processes such as rosette formation and mitosis as well as cell shape and motility. Imaging on retinal explants highlights single 3D cone and rod structures. An optimal workflow for data acquisition, postprocessing and saving is demonstrated, resulting in a time gain factor of 10 compared to prior state of the art. Finally, a method to increase D-FFOCT signal-to-noise ratio is demonstrated, allowing rapid organoid screening.https://doi.org/10.1038/s42003-023-05378-w |
| spellingShingle | Tual Monfort Salvatore Azzollini Jérémy Brogard Marilou Clémençon Amélie Slembrouck-Brec Valerie Forster Serge Picaud Olivier Goureau Sacha Reichman Olivier Thouvenin Kate Grieve Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study Communications Biology |
| title | Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study |
| title_full | Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study |
| title_fullStr | Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study |
| title_full_unstemmed | Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study |
| title_short | Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study |
| title_sort | dynamic full field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study |
| url | https://doi.org/10.1038/s42003-023-05378-w |
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