miR‐9 promotes canine endothelial‐like cell migration by targeting COL15A1
Abstract Background Endothelial cell migration is the initial stage of angiogenesis. In previous studies, miR‐9 has been found to regulate angiogenesis and cell migration in human medicine. Objectives This study aimed to reveal the regulatory effect of miR‐9 on canine endothelial cell migration. Met...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.1339 |
| _version_ | 1797353769000763392 |
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| author | Heng Jiang Mengmeng Liu Yao Qin Hong Zhang |
| author_facet | Heng Jiang Mengmeng Liu Yao Qin Hong Zhang |
| author_sort | Heng Jiang |
| collection | DOAJ |
| description | Abstract Background Endothelial cell migration is the initial stage of angiogenesis. In previous studies, miR‐9 has been found to regulate angiogenesis and cell migration in human medicine. Objectives This study aimed to reveal the regulatory effect of miR‐9 on canine endothelial cell migration. Methods Embryonic canine ventricle myocardium tissues were collected and induced to differentiate into endothelial‐like cells (ELCs). A transwell and invasion assay were used to evaluate the impact of miR‐9 on the migration capacity of ELCs, after which a luciferase reporter assay, western blotting, RNA sequencing and reverse transcription‐polymerase chain reaction were conducted to explore the regulatory mechanism. Results Our results showed that we successfully induced the primary cells derived from canine cardiac embryo tissues into ELCs. MiR‐9 also promoted the migration and invasion of canine ELCs, and inhibited the expression of collagen XV, an angiogenic inhibitor, at the translational level by targeting the 3′ untranslated region of COL15A1 gene. Furthermore, RNA sequencing showed that overexpression of miR‐9 impacted several signalling pathways and eight genes involved in angiogenesis and cell migration in canine ELCs. Conclusions These findings suggest that miR‐9 enhances the migration of canine ELCs and may serve as a potential diagnostic and therapeutic target for canine diseases involved in endothelial cells migration and angiogenesis, but more further studies are needed. |
| first_indexed | 2024-03-08T13:35:48Z |
| format | Article |
| id | doaj.art-d7c65f6982d54d7988d6809c637be1e2 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-08T13:35:48Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-d7c65f6982d54d7988d6809c637be1e22024-01-16T18:58:33ZengWileyVeterinary Medicine and Science2053-10952024-01-01101n/an/a10.1002/vms3.1339miR‐9 promotes canine endothelial‐like cell migration by targeting COL15A1Heng Jiang0Mengmeng Liu1Yao Qin2Hong Zhang3Institute of Tropical Agriculture and Forestry Hainan University Hainan PR ChinaInstitute of Tropical Agriculture and Forestry Hainan University Hainan PR ChinaInstitute of Tropical Agriculture and Forestry Hainan University Hainan PR ChinaInstitute of Tropical Agriculture and Forestry Hainan University Hainan PR ChinaAbstract Background Endothelial cell migration is the initial stage of angiogenesis. In previous studies, miR‐9 has been found to regulate angiogenesis and cell migration in human medicine. Objectives This study aimed to reveal the regulatory effect of miR‐9 on canine endothelial cell migration. Methods Embryonic canine ventricle myocardium tissues were collected and induced to differentiate into endothelial‐like cells (ELCs). A transwell and invasion assay were used to evaluate the impact of miR‐9 on the migration capacity of ELCs, after which a luciferase reporter assay, western blotting, RNA sequencing and reverse transcription‐polymerase chain reaction were conducted to explore the regulatory mechanism. Results Our results showed that we successfully induced the primary cells derived from canine cardiac embryo tissues into ELCs. MiR‐9 also promoted the migration and invasion of canine ELCs, and inhibited the expression of collagen XV, an angiogenic inhibitor, at the translational level by targeting the 3′ untranslated region of COL15A1 gene. Furthermore, RNA sequencing showed that overexpression of miR‐9 impacted several signalling pathways and eight genes involved in angiogenesis and cell migration in canine ELCs. Conclusions These findings suggest that miR‐9 enhances the migration of canine ELCs and may serve as a potential diagnostic and therapeutic target for canine diseases involved in endothelial cells migration and angiogenesis, but more further studies are needed.https://doi.org/10.1002/vms3.1339canine endothelial‐like cellcell migrationCOL15A1collagen XVmiR‐9 |
| spellingShingle | Heng Jiang Mengmeng Liu Yao Qin Hong Zhang miR‐9 promotes canine endothelial‐like cell migration by targeting COL15A1 Veterinary Medicine and Science canine endothelial‐like cell cell migration COL15A1 collagen XV miR‐9 |
| title | miR‐9 promotes canine endothelial‐like cell migration by targeting COL15A1 |
| title_full | miR‐9 promotes canine endothelial‐like cell migration by targeting COL15A1 |
| title_fullStr | miR‐9 promotes canine endothelial‐like cell migration by targeting COL15A1 |
| title_full_unstemmed | miR‐9 promotes canine endothelial‐like cell migration by targeting COL15A1 |
| title_short | miR‐9 promotes canine endothelial‐like cell migration by targeting COL15A1 |
| title_sort | mir 9 promotes canine endothelial like cell migration by targeting col15a1 |
| topic | canine endothelial‐like cell cell migration COL15A1 collagen XV miR‐9 |
| url | https://doi.org/10.1002/vms3.1339 |
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