Detection of antibacterial protein in Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae)
Abstract Antimicrobial peptides (AMPs) constitute a major arm of defense in mosquitoes against microbes. The purpose of this study was to determine which of the peptides are produced in the mosquito hemolymph after bacterial treatment. Culex pipiens (Diptera: Culicidae) larvae, collected from draina...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2018-03-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s41938-018-0037-0 |
| _version_ | 1818262776928272384 |
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| author | Maha K. Tewfick Waleed M. Serag Belal A. Soliman |
| author_facet | Maha K. Tewfick Waleed M. Serag Belal A. Soliman |
| author_sort | Maha K. Tewfick |
| collection | DOAJ |
| description | Abstract Antimicrobial peptides (AMPs) constitute a major arm of defense in mosquitoes against microbes. The purpose of this study was to determine which of the peptides are produced in the mosquito hemolymph after bacterial treatment. Culex pipiens (Diptera: Culicidae) larvae, collected from drainage canal in Suez Governorate, Egypt, were treated with LC80 of Bacillus sphaericus strain 2362 (0.035 ppm). The hemolymph of bacteria-treated Cx. pipiens mosquitoes and non-treated mosquitoes as control were extracted and undergo electrophoresis, using a Bio-Rad Mini-protean II cell. In Cx. pipiens mosquitoes emerging from bacteria-treated larvae, a band of protein was detected at 165 kDa. This protein was more likely to be Thioester-containing protein 1 (TEP1). Understanding mosquito resistance mechanism to bacterial control is crucial to build up programs to overcome such resistance. |
| first_indexed | 2024-12-12T19:08:30Z |
| format | Article |
| id | doaj.art-d7cc6b67863545658ffdecce2816a7de |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-12-12T19:08:30Z |
| publishDate | 2018-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-d7cc6b67863545658ffdecce2816a7de2022-12-22T00:14:55ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422018-03-012811610.1186/s41938-018-0037-0Detection of antibacterial protein in Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae)Maha K. Tewfick0Waleed M. Serag1Belal A. Soliman2Department of Zoology, Faculty of Science, Suez UniversityDepartment of Chemistry, Faculty of Science, Suez UniversityDepartment of Zoology, Faculty of Science, Suez UniversityAbstract Antimicrobial peptides (AMPs) constitute a major arm of defense in mosquitoes against microbes. The purpose of this study was to determine which of the peptides are produced in the mosquito hemolymph after bacterial treatment. Culex pipiens (Diptera: Culicidae) larvae, collected from drainage canal in Suez Governorate, Egypt, were treated with LC80 of Bacillus sphaericus strain 2362 (0.035 ppm). The hemolymph of bacteria-treated Cx. pipiens mosquitoes and non-treated mosquitoes as control were extracted and undergo electrophoresis, using a Bio-Rad Mini-protean II cell. In Cx. pipiens mosquitoes emerging from bacteria-treated larvae, a band of protein was detected at 165 kDa. This protein was more likely to be Thioester-containing protein 1 (TEP1). Understanding mosquito resistance mechanism to bacterial control is crucial to build up programs to overcome such resistance.http://link.springer.com/article/10.1186/s41938-018-0037-0Antibacterial proteinBacillus sphaericusCulex pipiens |
| spellingShingle | Maha K. Tewfick Waleed M. Serag Belal A. Soliman Detection of antibacterial protein in Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae) Egyptian Journal of Biological Pest Control Antibacterial protein Bacillus sphaericus Culex pipiens |
| title | Detection of antibacterial protein in Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae) |
| title_full | Detection of antibacterial protein in Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae) |
| title_fullStr | Detection of antibacterial protein in Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae) |
| title_full_unstemmed | Detection of antibacterial protein in Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae) |
| title_short | Detection of antibacterial protein in Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae) |
| title_sort | detection of antibacterial protein in bacillus sphaericus treated culex pipiens diptera culicidae |
| topic | Antibacterial protein Bacillus sphaericus Culex pipiens |
| url | http://link.springer.com/article/10.1186/s41938-018-0037-0 |
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