High-parameter immune profiling and subjective health assessment of the immunomodulatory effects of paramylon-rich Euglena gracilis EOD-1: A randomized, double-blind, placebo-controlled, parallel-group study
Paramylon, a characteristic β-glucan of microalgae Euglena gracilis, modulates immune function. However, data on its mechanism of action and use in humans are limited. We conducted a 12-week randomized controlled trial to determine the effects of paramylon-rich E. gracilis EOD-1 (PM EOD-1) ingestion...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623004048 |
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| author | Takanori Kawano Atsushi Miura Junko Naito Norihisa Nishida Ken-ichi Ishibashi Yoshiyuki Adachi Naohito Ohno Yuji Naito |
| author_facet | Takanori Kawano Atsushi Miura Junko Naito Norihisa Nishida Ken-ichi Ishibashi Yoshiyuki Adachi Naohito Ohno Yuji Naito |
| author_sort | Takanori Kawano |
| collection | DOAJ |
| description | Paramylon, a characteristic β-glucan of microalgae Euglena gracilis, modulates immune function. However, data on its mechanism of action and use in humans are limited. We conducted a 12-week randomized controlled trial to determine the effects of paramylon-rich E. gracilis EOD-1 (PM EOD-1) ingestion on immune function and physical condition by randomly assigning 24 healthy men and women (50–65 years old) to PM EOD-1 (PM 350 mg) or placebo groups. We evaluated physical condition, cytokine proteomics, and high-parameter immune profiling. The PM EOD-1 group exhibited a higher number of cumulative good health days, increased or sustained high IL17A, IL27, CSF2, FLT3LG, and IL17C levels, increased CD28 expression in CD4+/CD8+ naïve T cells, and maintained CD80 expression in monocytes than the placebo group. PM EOD-1 promotes immune cell response potential by enhancing monocyte activation and co-stimulatory molecule interactions between T and antigen-presenting cells, thereby promoting immune defense and good health. |
| first_indexed | 2024-03-11T18:29:21Z |
| format | Article |
| id | doaj.art-d7d1029f57e4416b8ff5560f04e2484d |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-11T18:29:21Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-d7d1029f57e4416b8ff5560f04e2484d2023-10-13T13:52:55ZengElsevierJournal of Functional Foods1756-46462023-10-01109105804High-parameter immune profiling and subjective health assessment of the immunomodulatory effects of paramylon-rich Euglena gracilis EOD-1: A randomized, double-blind, placebo-controlled, parallel-group studyTakanori Kawano0Atsushi Miura1Junko Naito2Norihisa Nishida3Ken-ichi Ishibashi4Yoshiyuki Adachi5Naohito Ohno6Yuji Naito7Kobelco Eco-Solutions Co., Ltd, 1-1-4 Murotani, Nishi-ku, Kobe, Hyogo 650-2241, Japan; Corresponding author.IMEQRD Co., Ltd, 6-2-1 Ginza, Chuo-ku, Tokyo 104-0061, JapanKobelco Eco-Solutions Co., Ltd, 1-1-4 Murotani, Nishi-ku, Kobe, Hyogo 650-2241, JapanKobelco Eco-Solutions Co., Ltd, 1-1-4 Murotani, Nishi-ku, Kobe, Hyogo 650-2241, JapanLaboratory of Host Defense and Responses, Faculty of Nutrition, Kagawa Nutrition University, 3–9–21 Chiyoda, Sakado, Saitama 350–0288, JapanLaboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432–1 Horinouchi, Hachioji, Tokyo 192–0392, JapanLaboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432–1 Horinouchi, Hachioji, Tokyo 192–0392, JapanDepartment of Human Immunology and Nutrition Science, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, JapanParamylon, a characteristic β-glucan of microalgae Euglena gracilis, modulates immune function. However, data on its mechanism of action and use in humans are limited. We conducted a 12-week randomized controlled trial to determine the effects of paramylon-rich E. gracilis EOD-1 (PM EOD-1) ingestion on immune function and physical condition by randomly assigning 24 healthy men and women (50–65 years old) to PM EOD-1 (PM 350 mg) or placebo groups. We evaluated physical condition, cytokine proteomics, and high-parameter immune profiling. The PM EOD-1 group exhibited a higher number of cumulative good health days, increased or sustained high IL17A, IL27, CSF2, FLT3LG, and IL17C levels, increased CD28 expression in CD4+/CD8+ naïve T cells, and maintained CD80 expression in monocytes than the placebo group. PM EOD-1 promotes immune cell response potential by enhancing monocyte activation and co-stimulatory molecule interactions between T and antigen-presenting cells, thereby promoting immune defense and good health.http://www.sciencedirect.com/science/article/pii/S1756464623004048Euglnena gracilis EOD-1Paramylonβ-glucanImmune functionAntigen presentationMass cytometry |
| spellingShingle | Takanori Kawano Atsushi Miura Junko Naito Norihisa Nishida Ken-ichi Ishibashi Yoshiyuki Adachi Naohito Ohno Yuji Naito High-parameter immune profiling and subjective health assessment of the immunomodulatory effects of paramylon-rich Euglena gracilis EOD-1: A randomized, double-blind, placebo-controlled, parallel-group study Journal of Functional Foods Euglnena gracilis EOD-1 Paramylon β-glucan Immune function Antigen presentation Mass cytometry |
| title | High-parameter immune profiling and subjective health assessment of the immunomodulatory effects of paramylon-rich Euglena gracilis EOD-1: A randomized, double-blind, placebo-controlled, parallel-group study |
| title_full | High-parameter immune profiling and subjective health assessment of the immunomodulatory effects of paramylon-rich Euglena gracilis EOD-1: A randomized, double-blind, placebo-controlled, parallel-group study |
| title_fullStr | High-parameter immune profiling and subjective health assessment of the immunomodulatory effects of paramylon-rich Euglena gracilis EOD-1: A randomized, double-blind, placebo-controlled, parallel-group study |
| title_full_unstemmed | High-parameter immune profiling and subjective health assessment of the immunomodulatory effects of paramylon-rich Euglena gracilis EOD-1: A randomized, double-blind, placebo-controlled, parallel-group study |
| title_short | High-parameter immune profiling and subjective health assessment of the immunomodulatory effects of paramylon-rich Euglena gracilis EOD-1: A randomized, double-blind, placebo-controlled, parallel-group study |
| title_sort | high parameter immune profiling and subjective health assessment of the immunomodulatory effects of paramylon rich euglena gracilis eod 1 a randomized double blind placebo controlled parallel group study |
| topic | Euglnena gracilis EOD-1 Paramylon β-glucan Immune function Antigen presentation Mass cytometry |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623004048 |
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