Aerosolization behavior of antimicrobial resistance in animal farms: a field study from feces to fine particulate matter
Antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB) in animal feces can be released into the atmosphere via aerosolization, posing a high health risk to farm workers. So far, little attention has been paid to the characterization of the aerosolization process. In this study, f...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1175265/full |
| _version_ | 1797843740698607616 |
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| author | Huibo Xin Huibo Xin Tianlei Qiu Yajie Guo Haoze Gao Liqiu Zhang Min Gao |
| author_facet | Huibo Xin Huibo Xin Tianlei Qiu Yajie Guo Haoze Gao Liqiu Zhang Min Gao |
| author_sort | Huibo Xin |
| collection | DOAJ |
| description | Antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB) in animal feces can be released into the atmosphere via aerosolization, posing a high health risk to farm workers. So far, little attention has been paid to the characterization of the aerosolization process. In this study, fecal and fine particulate matter (PM2.5) samples were collected from 20 animal farms involving swine, cattle, layers, and broilers, and the ARGs, ARB, and human pathogenic bacteria (HPB) were loaded in these two media. The results showed that approximately 70% of ARGs, 60% of ARBs, and 43% of HPBs were found to be preferential aerosolization. The bioaerosolization index (BI) of target 30 ARGs varied from 0.04 to 460.07, and the highest value was detected from tetW. The highest BI values of erythromycin- and tetracycline-resistant bacteria were for Kocuria (13119) and Staphylococcus (24746), respectively, and the distribution of BI in the two types of dominant ARB was similar. Regarding the bioaerosolization behavior of HPB, Clostridium saccharolyticum WM1 was the most easily aerosolized pathogen in swine and broiler farms, and Brucella abortus strain CNM 20040339 had the highest value in cattle and layer farms. Notably, the highest BI values for ARGs, ARB, and HPB were universally detected on chicken farms. Most ARGs, ARB, and HPB positively correlated with animal age, stocking density, and breeding area. Temperature and relative humidity have significant effects on the aerosolization behavior of targets, and the effects of these two parameters on the same target are usually opposite. The results of this study provide a basis for a better understanding of the contribution of animal feces to airborne ARGs and HPBs in farms, as well as for controlling the transport of the fecal microbiome to the environment through the aerosolization pathway. |
| first_indexed | 2024-04-09T17:11:58Z |
| format | Article |
| id | doaj.art-d7d1c042ca14425095989917718e2549 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T17:11:58Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d7d1c042ca14425095989917718e25492023-04-20T05:58:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11752651175265Aerosolization behavior of antimicrobial resistance in animal farms: a field study from feces to fine particulate matterHuibo Xin0Huibo Xin1Tianlei Qiu2Yajie Guo3Haoze Gao4Liqiu Zhang5Min Gao6Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory for Source Control Technology of Water Pollution, Engineering Research Center for Water Pollution Source Control and Eco-remediation, Beijing Forestry University, Beijing, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory for Source Control Technology of Water Pollution, Engineering Research Center for Water Pollution Source Control and Eco-remediation, Beijing Forestry University, Beijing, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaAntibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB) in animal feces can be released into the atmosphere via aerosolization, posing a high health risk to farm workers. So far, little attention has been paid to the characterization of the aerosolization process. In this study, fecal and fine particulate matter (PM2.5) samples were collected from 20 animal farms involving swine, cattle, layers, and broilers, and the ARGs, ARB, and human pathogenic bacteria (HPB) were loaded in these two media. The results showed that approximately 70% of ARGs, 60% of ARBs, and 43% of HPBs were found to be preferential aerosolization. The bioaerosolization index (BI) of target 30 ARGs varied from 0.04 to 460.07, and the highest value was detected from tetW. The highest BI values of erythromycin- and tetracycline-resistant bacteria were for Kocuria (13119) and Staphylococcus (24746), respectively, and the distribution of BI in the two types of dominant ARB was similar. Regarding the bioaerosolization behavior of HPB, Clostridium saccharolyticum WM1 was the most easily aerosolized pathogen in swine and broiler farms, and Brucella abortus strain CNM 20040339 had the highest value in cattle and layer farms. Notably, the highest BI values for ARGs, ARB, and HPB were universally detected on chicken farms. Most ARGs, ARB, and HPB positively correlated with animal age, stocking density, and breeding area. Temperature and relative humidity have significant effects on the aerosolization behavior of targets, and the effects of these two parameters on the same target are usually opposite. The results of this study provide a basis for a better understanding of the contribution of animal feces to airborne ARGs and HPBs in farms, as well as for controlling the transport of the fecal microbiome to the environment through the aerosolization pathway.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1175265/fullanimal farmsfine particulate matterantibiotic resistancehuman pathogenic bacteriaaerosolization behavior |
| spellingShingle | Huibo Xin Huibo Xin Tianlei Qiu Yajie Guo Haoze Gao Liqiu Zhang Min Gao Aerosolization behavior of antimicrobial resistance in animal farms: a field study from feces to fine particulate matter Frontiers in Microbiology animal farms fine particulate matter antibiotic resistance human pathogenic bacteria aerosolization behavior |
| title | Aerosolization behavior of antimicrobial resistance in animal farms: a field study from feces to fine particulate matter |
| title_full | Aerosolization behavior of antimicrobial resistance in animal farms: a field study from feces to fine particulate matter |
| title_fullStr | Aerosolization behavior of antimicrobial resistance in animal farms: a field study from feces to fine particulate matter |
| title_full_unstemmed | Aerosolization behavior of antimicrobial resistance in animal farms: a field study from feces to fine particulate matter |
| title_short | Aerosolization behavior of antimicrobial resistance in animal farms: a field study from feces to fine particulate matter |
| title_sort | aerosolization behavior of antimicrobial resistance in animal farms a field study from feces to fine particulate matter |
| topic | animal farms fine particulate matter antibiotic resistance human pathogenic bacteria aerosolization behavior |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1175265/full |
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