Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity
Abstract The chylous turbidity of blood samples is one of the causes of false-high hemoglobin (Hgb) concentration measurements by the colorimetric method, which has been widely applied in hematology analyzers. In such cases, additional manual procedures are required to correct Hgb concentrations. We...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
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Nature Portfolio
2021-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-94394-z |
| _version_ | 1818752046146256896 |
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| author | Yu Aruga Chiaki Ikeda Arisa Hanai Sakiko Yoshimura Momoko Kito Satoe Miyaki Misato Tsubokura Yuka Yasuno Chiaki Hayashi Motoi Miyakoshi Takahiro Nishino Kimihiko Kawamura Hiromichi Matsushita |
| author_facet | Yu Aruga Chiaki Ikeda Arisa Hanai Sakiko Yoshimura Momoko Kito Satoe Miyaki Misato Tsubokura Yuka Yasuno Chiaki Hayashi Motoi Miyakoshi Takahiro Nishino Kimihiko Kawamura Hiromichi Matsushita |
| author_sort | Yu Aruga |
| collection | DOAJ |
| description | Abstract The chylous turbidity of blood samples is one of the causes of false-high hemoglobin (Hgb) concentration measurements by the colorimetric method, which has been widely applied in hematology analyzers. In such cases, additional manual procedures are required to correct Hgb concentrations. We therefore examined the effectiveness of an optical method for measuring Hgb concentrations in samples with chylous turbidity using Hgb-O in the reticulocyte channel equipped in XN-series analyzers (Sysmex, Kobe, Japan). Hgb-O showed excellent basic performance, including linear correlation and invariability with sodium lauryl sulfate (SLS)-Hgb detected by the colorimetric method. In the analysis of samples from healthy volunteers supplemented with fat emulsion, chylous turbidity did not affect Hgb-O but SLS-Hgb, which was falsely increased according to the dose of fat emulsion. Actually, SLS-Hgb was falsely elevated in 34 of 40 chylous turbidity 3+ samples. The remaining 6 samples were measured in hematology analyzers where Hgb-O was inconsistent with SLS-Hgb in the internal quality control records. For these samples, the correction factors calculated from the internal quality control records could contribute to providing the corrected Hgb-O value. These findings suggested that the optical method was effective and convenient for accurately evaluating Hgb concentrations in samples with extremely chylous turbidity. |
| first_indexed | 2024-12-18T04:45:14Z |
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| id | doaj.art-d7d25f2affa04020b008fc99a79005bb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:45:14Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-d7d25f2affa04020b008fc99a79005bb2022-12-21T21:20:37ZengNature PortfolioScientific Reports2045-23222021-07-011111910.1038/s41598-021-94394-zConvenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidityYu Aruga0Chiaki Ikeda1Arisa Hanai2Sakiko Yoshimura3Momoko Kito4Satoe Miyaki5Misato Tsubokura6Yuka Yasuno7Chiaki Hayashi8Motoi Miyakoshi9Takahiro Nishino10Kimihiko Kawamura11Hiromichi Matsushita12Department of Laboratory Medicine, National Cancer Center HospitalDepartment of Laboratory Medicine, National Cancer Center HospitalDepartment of Laboratory Medicine, National Cancer Center HospitalDepartment of Laboratory Medicine, National Cancer Center HospitalDepartment of Laboratory Medicine, National Cancer Center HospitalDepartment of Laboratory Medicine, National Cancer Center HospitalDepartment of Laboratory Medicine, National Cancer Center HospitalDepartment of Laboratory Medicine, National Cancer Center HospitalDepartment of Laboratory Medicine, National Cancer Center HospitalDepartment of Laboratory Medicine, National Cancer Center HospitalDepartment of Laboratory Medicine, National Cancer Center HospitalDepartment of Laboratory Medicine, National Cancer Center HospitalDepartment of Laboratory Medicine, National Cancer Center HospitalAbstract The chylous turbidity of blood samples is one of the causes of false-high hemoglobin (Hgb) concentration measurements by the colorimetric method, which has been widely applied in hematology analyzers. In such cases, additional manual procedures are required to correct Hgb concentrations. We therefore examined the effectiveness of an optical method for measuring Hgb concentrations in samples with chylous turbidity using Hgb-O in the reticulocyte channel equipped in XN-series analyzers (Sysmex, Kobe, Japan). Hgb-O showed excellent basic performance, including linear correlation and invariability with sodium lauryl sulfate (SLS)-Hgb detected by the colorimetric method. In the analysis of samples from healthy volunteers supplemented with fat emulsion, chylous turbidity did not affect Hgb-O but SLS-Hgb, which was falsely increased according to the dose of fat emulsion. Actually, SLS-Hgb was falsely elevated in 34 of 40 chylous turbidity 3+ samples. The remaining 6 samples were measured in hematology analyzers where Hgb-O was inconsistent with SLS-Hgb in the internal quality control records. For these samples, the correction factors calculated from the internal quality control records could contribute to providing the corrected Hgb-O value. These findings suggested that the optical method was effective and convenient for accurately evaluating Hgb concentrations in samples with extremely chylous turbidity.https://doi.org/10.1038/s41598-021-94394-z |
| spellingShingle | Yu Aruga Chiaki Ikeda Arisa Hanai Sakiko Yoshimura Momoko Kito Satoe Miyaki Misato Tsubokura Yuka Yasuno Chiaki Hayashi Motoi Miyakoshi Takahiro Nishino Kimihiko Kawamura Hiromichi Matsushita Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity Scientific Reports |
| title | Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity |
| title_full | Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity |
| title_fullStr | Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity |
| title_full_unstemmed | Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity |
| title_short | Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity |
| title_sort | convenience of hgb o detected by optical method in xn series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity |
| url | https://doi.org/10.1038/s41598-021-94394-z |
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