Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involved
Objective(s): This study was undertaken to investigate the efficacy of telomerase activator-65 (Ta-65) and pomegranate peel against aging-induced deteriorations in the liver. Materials and Methods: The rats were divided into four groups: control, aged, aged rats treated with Ta-65, and pomegranate o...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2021-06-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_18137_0b44e7185b267567d3232a551c621f0d.pdf |
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| author | Ameera Alshinnawy Wael El-Sayed Ahmed Sayed Ahmed Salem AlShaimaa Taha |
| author_facet | Ameera Alshinnawy Wael El-Sayed Ahmed Sayed Ahmed Salem AlShaimaa Taha |
| author_sort | Ameera Alshinnawy |
| collection | DOAJ |
| description | Objective(s): This study was undertaken to investigate the efficacy of telomerase activator-65 (Ta-65) and pomegranate peel against aging-induced deteriorations in the liver. Materials and Methods: The rats were divided into four groups: control, aged, aged rats treated with Ta-65, and pomegranate orally for two months.Results: Aging significantly increased the serum levels of total protein, globulins, and protein carbonyl and reduced the insulin-like growth factor 1 (IGF-1). It also elevated the hepatic malondialdehyde and decreased the hepatic glutathione S-transferase (GST) activity. Aging elevated the expression of thioredoxin reductase1, telomerase reverse transcriptase, and cytochrome 3a1 in the liver; it increased the p53 protein level and elevated the activity of caspase-3 in the liver indicating the occurrence of apoptosis. The architecture of the liver deteriorated in the aged rats, as shown by both light and electron microscopy examinations. The liver of the aged rats had many apoptotic hepatocytes with shrunken nuclei. Many hepatocytes had dilated rough endoplasmic reticulum, many lysosomes, and many fat droplets. Administration of Ta-65 and pomegranate to the aged rats normalized most of the previous biochemical parameters and improved the liver architecture. Conclusion: Ta-65 and pomegranate have anti-aging activity through targeting multiple cellular pathways. It is also noteworthy that Ta-65 was superior to pomegranate in its alleviative effects. |
| first_indexed | 2024-12-19T17:17:59Z |
| format | Article |
| id | doaj.art-d7d761283be84ad29957d2c0f344f578 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-19T17:17:59Z |
| publishDate | 2021-06-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-d7d761283be84ad29957d2c0f344f5782022-12-21T20:12:49ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742021-06-0124684285010.22038/ijbms.2021.56670.1265518137Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involvedAmeera Alshinnawy0Wael El-Sayed1Ahmed Sayed2Ahmed Salem3AlShaimaa Taha4Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt. 11566Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt. 11566Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt. 11566Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt. 11566Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt. 11566Objective(s): This study was undertaken to investigate the efficacy of telomerase activator-65 (Ta-65) and pomegranate peel against aging-induced deteriorations in the liver. Materials and Methods: The rats were divided into four groups: control, aged, aged rats treated with Ta-65, and pomegranate orally for two months.Results: Aging significantly increased the serum levels of total protein, globulins, and protein carbonyl and reduced the insulin-like growth factor 1 (IGF-1). It also elevated the hepatic malondialdehyde and decreased the hepatic glutathione S-transferase (GST) activity. Aging elevated the expression of thioredoxin reductase1, telomerase reverse transcriptase, and cytochrome 3a1 in the liver; it increased the p53 protein level and elevated the activity of caspase-3 in the liver indicating the occurrence of apoptosis. The architecture of the liver deteriorated in the aged rats, as shown by both light and electron microscopy examinations. The liver of the aged rats had many apoptotic hepatocytes with shrunken nuclei. Many hepatocytes had dilated rough endoplasmic reticulum, many lysosomes, and many fat droplets. Administration of Ta-65 and pomegranate to the aged rats normalized most of the previous biochemical parameters and improved the liver architecture. Conclusion: Ta-65 and pomegranate have anti-aging activity through targeting multiple cellular pathways. It is also noteworthy that Ta-65 was superior to pomegranate in its alleviative effects.https://ijbms.mums.ac.ir/article_18137_0b44e7185b267567d3232a551c621f0d.pdfagingapoptosisigf1oxidative stresstelomerasethioredoxin reductase |
| spellingShingle | Ameera Alshinnawy Wael El-Sayed Ahmed Sayed Ahmed Salem AlShaimaa Taha Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involved Iranian Journal of Basic Medical Sciences aging apoptosis igf1 oxidative stress telomerase thioredoxin reductase |
| title | Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involved |
| title_full | Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involved |
| title_fullStr | Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involved |
| title_full_unstemmed | Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involved |
| title_short | Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involved |
| title_sort | telomerase activator 65 and pomegranate peel improved the health status of the liver in aged rats multi targets involved |
| topic | aging apoptosis igf1 oxidative stress telomerase thioredoxin reductase |
| url | https://ijbms.mums.ac.ir/article_18137_0b44e7185b267567d3232a551c621f0d.pdf |
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