The Core Jasmonic Acid-Signalling Module <i>CoCOI1/CoJAZ1/CoMYC2</i> Are Involved in Jas Mediated Growth of the Pollen Tube in <i>Camellia oleifera</i>
<i>Camellia oleifera</i> is a woody edible oil species with late self-incompatibility characteristics. Previous transcriptome analysis showed that genes involved in jasmonic acid signal transduction were significantly different in self-and cross-pollinated pistils of <i>Camellia ol...
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MDPI AG
2022-11-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/11/366 |
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| author | Yiyao Liu Junqin Zhou Mengqi Lu Jin Yang Xiaofeng Tan |
| author_facet | Yiyao Liu Junqin Zhou Mengqi Lu Jin Yang Xiaofeng Tan |
| author_sort | Yiyao Liu |
| collection | DOAJ |
| description | <i>Camellia oleifera</i> is a woody edible oil species with late self-incompatibility characteristics. Previous transcriptome analysis showed that genes involved in jasmonic acid signal transduction were significantly different in self-and cross-pollinated pistils of <i>Camellia oleifera</i>. To investigate the relationship between jasmonate signal and self-incompatibility by studying the core genes of jasmonate signal transduction. The results showed that exogenous JA and MeJA at 1.0 mM significantly inhibited pollen tube germination and pollen tube elongation. and JA up-regulated <i>CoCOI1</i>, <i>CoJAZ1</i>, and <i>CoMYC</i>, the core genes of jasmonate signal transduction. Subcellular localization indicated that CoCOI1 and CoJAZ1 were located in the nucleus and CoMYC2 in the endoplasmic reticulum. The three genes exhibited tissue-specific expression pattern. <i>CoCOI1</i> was significantly expressed in pollen, <i>CoJAZ1</i> was significantly expressed in ovary, <i>CoMYC2</i> was significantly expressed in filaments, but not in pollen. Furthermore, <i>CoJAZ1</i> and <i>CoMYC2</i> were highly expressing at 24 h in self-pollinated styles. These results suggested that JA signal transduction of <i>C. oleifera</i> was involved in the process of self-pollination, and thus in the process of plant defense. When pollen tubes grew slowly in the style, ovary may receive JA signal, which initiates the molecular mechanism of inhibiting the growth of self-pollinating pollen tubes. |
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| last_indexed | 2024-03-09T19:10:44Z |
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| spelling | doaj.art-d7da1977bcee459ca62281b81301267c2023-11-24T04:13:11ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-11-0144115405541510.3390/cimb44110366The Core Jasmonic Acid-Signalling Module <i>CoCOI1/CoJAZ1/CoMYC2</i> Are Involved in Jas Mediated Growth of the Pollen Tube in <i>Camellia oleifera</i>Yiyao Liu0Junqin Zhou1Mengqi Lu2Jin Yang3Xiaofeng Tan4Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410001, ChinaKey Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410001, ChinaKey Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410001, ChinaKey Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410001, ChinaKey Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410001, China<i>Camellia oleifera</i> is a woody edible oil species with late self-incompatibility characteristics. Previous transcriptome analysis showed that genes involved in jasmonic acid signal transduction were significantly different in self-and cross-pollinated pistils of <i>Camellia oleifera</i>. To investigate the relationship between jasmonate signal and self-incompatibility by studying the core genes of jasmonate signal transduction. The results showed that exogenous JA and MeJA at 1.0 mM significantly inhibited pollen tube germination and pollen tube elongation. and JA up-regulated <i>CoCOI1</i>, <i>CoJAZ1</i>, and <i>CoMYC</i>, the core genes of jasmonate signal transduction. Subcellular localization indicated that CoCOI1 and CoJAZ1 were located in the nucleus and CoMYC2 in the endoplasmic reticulum. The three genes exhibited tissue-specific expression pattern. <i>CoCOI1</i> was significantly expressed in pollen, <i>CoJAZ1</i> was significantly expressed in ovary, <i>CoMYC2</i> was significantly expressed in filaments, but not in pollen. Furthermore, <i>CoJAZ1</i> and <i>CoMYC2</i> were highly expressing at 24 h in self-pollinated styles. These results suggested that JA signal transduction of <i>C. oleifera</i> was involved in the process of self-pollination, and thus in the process of plant defense. When pollen tubes grew slowly in the style, ovary may receive JA signal, which initiates the molecular mechanism of inhibiting the growth of self-pollinating pollen tubes.https://www.mdpi.com/1467-3045/44/11/366<i>Camellia</i> oil treeself-incompatibilityjasmonic acidexpression |
| spellingShingle | Yiyao Liu Junqin Zhou Mengqi Lu Jin Yang Xiaofeng Tan The Core Jasmonic Acid-Signalling Module <i>CoCOI1/CoJAZ1/CoMYC2</i> Are Involved in Jas Mediated Growth of the Pollen Tube in <i>Camellia oleifera</i> Current Issues in Molecular Biology <i>Camellia</i> oil tree self-incompatibility jasmonic acid expression |
| title | The Core Jasmonic Acid-Signalling Module <i>CoCOI1/CoJAZ1/CoMYC2</i> Are Involved in Jas Mediated Growth of the Pollen Tube in <i>Camellia oleifera</i> |
| title_full | The Core Jasmonic Acid-Signalling Module <i>CoCOI1/CoJAZ1/CoMYC2</i> Are Involved in Jas Mediated Growth of the Pollen Tube in <i>Camellia oleifera</i> |
| title_fullStr | The Core Jasmonic Acid-Signalling Module <i>CoCOI1/CoJAZ1/CoMYC2</i> Are Involved in Jas Mediated Growth of the Pollen Tube in <i>Camellia oleifera</i> |
| title_full_unstemmed | The Core Jasmonic Acid-Signalling Module <i>CoCOI1/CoJAZ1/CoMYC2</i> Are Involved in Jas Mediated Growth of the Pollen Tube in <i>Camellia oleifera</i> |
| title_short | The Core Jasmonic Acid-Signalling Module <i>CoCOI1/CoJAZ1/CoMYC2</i> Are Involved in Jas Mediated Growth of the Pollen Tube in <i>Camellia oleifera</i> |
| title_sort | core jasmonic acid signalling module i cocoi1 cojaz1 comyc2 i are involved in jas mediated growth of the pollen tube in i camellia oleifera i |
| topic | <i>Camellia</i> oil tree self-incompatibility jasmonic acid expression |
| url | https://www.mdpi.com/1467-3045/44/11/366 |
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