Study on Anti-cancer Effect of Plant Extract ACA-1 on Gastric Adenocarcinoma Cell Line
Background: Apoptosis provides solutions for the treatment of cancers and its induction is used as a strategy for preparing drugs that destroy pre-neoplastic cells. Many plant compounds have anti-tumoral activity. ACA-1 plant product is an aqueous extract and has been used in traditional medicine in...
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2012-05-01
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| Series: | Journal of Medicinal Plants |
| Subjects: | |
| Online Access: | http://jmp.ir/article-1-172-en.html |
| _version_ | 1818559640440406016 |
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| author | T Ghazanfari Sh Zamani Taghizadeh Rabe R Yaraee M Mahmoudi |
| author_facet | T Ghazanfari Sh Zamani Taghizadeh Rabe R Yaraee M Mahmoudi |
| author_sort | T Ghazanfari |
| collection | DOAJ |
| description | Background: Apoptosis provides solutions for the treatment of cancers and its induction is used as a strategy for preparing drugs that destroy pre-neoplastic cells. Many plant compounds have anti-tumoral activity. ACA-1 plant product is an aqueous extract and has been used in traditional medicine in Iran and has cytotoxicity effect on melanoma cancer cells. Objective: In this study, anti-cancer effect of ACA-1 plant product on gastric adenocarcinoma cells was evaluated and the mechanism of its action was studied. Methods: Cytotoxicity of ACA-1 on gastric adenocarcinoma cells (AGS) and fibroblasts (HgF) was determined after 24 hours of incubation by MTT assay. Annexin V-FITC and PI staining method was used for measuring the cell apoptosis. Activity of aspase 8 and 9 was assayed by enzymatic method. An ECMatrix was used for determining invasion ability of AGS cells. Results: ACA-1 showed strong and dose - dependent toxicity on AGS cells by induction of early apoptosis. Increase in caspase 8 and 9 activities was involved in this process. Also, ACA-1 decrease the invasion ability on AGS cells. Conclusion: ACA-1 induced apoptosis in human gastric cancer cells by activating caspase 8 and 9. With respect to decreasing cell invasion of AGS cells, ACA-1 may be considered as a potential candidate against human gastric cancer. |
| first_indexed | 2024-12-14T00:28:10Z |
| format | Article |
| id | doaj.art-d7dcf441e67d4ca2a85554f0cbef7bac |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-14T00:28:10Z |
| publishDate | 2012-05-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-d7dcf441e67d4ca2a85554f0cbef7bac2022-12-21T23:24:58ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582012-05-011142154162Study on Anti-cancer Effect of Plant Extract ACA-1 on Gastric Adenocarcinoma Cell LineT Ghazanfari0Sh Zamani Taghizadeh Rabe1R Yaraee2M Mahmoudi3 Department of Immunology, Faculty of Medicine, Shahed University Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences Department of Immunology, Faculty of Medicine, Shahed University Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences Background: Apoptosis provides solutions for the treatment of cancers and its induction is used as a strategy for preparing drugs that destroy pre-neoplastic cells. Many plant compounds have anti-tumoral activity. ACA-1 plant product is an aqueous extract and has been used in traditional medicine in Iran and has cytotoxicity effect on melanoma cancer cells. Objective: In this study, anti-cancer effect of ACA-1 plant product on gastric adenocarcinoma cells was evaluated and the mechanism of its action was studied. Methods: Cytotoxicity of ACA-1 on gastric adenocarcinoma cells (AGS) and fibroblasts (HgF) was determined after 24 hours of incubation by MTT assay. Annexin V-FITC and PI staining method was used for measuring the cell apoptosis. Activity of aspase 8 and 9 was assayed by enzymatic method. An ECMatrix was used for determining invasion ability of AGS cells. Results: ACA-1 showed strong and dose - dependent toxicity on AGS cells by induction of early apoptosis. Increase in caspase 8 and 9 activities was involved in this process. Also, ACA-1 decrease the invasion ability on AGS cells. Conclusion: ACA-1 induced apoptosis in human gastric cancer cells by activating caspase 8 and 9. With respect to decreasing cell invasion of AGS cells, ACA-1 may be considered as a potential candidate against human gastric cancer.http://jmp.ir/article-1-172-en.htmlcanceraca-1apoptosiscaspase |
| spellingShingle | T Ghazanfari Sh Zamani Taghizadeh Rabe R Yaraee M Mahmoudi Study on Anti-cancer Effect of Plant Extract ACA-1 on Gastric Adenocarcinoma Cell Line Journal of Medicinal Plants cancer aca-1 apoptosis caspase |
| title | Study on Anti-cancer Effect of Plant Extract ACA-1 on Gastric Adenocarcinoma Cell Line |
| title_full | Study on Anti-cancer Effect of Plant Extract ACA-1 on Gastric Adenocarcinoma Cell Line |
| title_fullStr | Study on Anti-cancer Effect of Plant Extract ACA-1 on Gastric Adenocarcinoma Cell Line |
| title_full_unstemmed | Study on Anti-cancer Effect of Plant Extract ACA-1 on Gastric Adenocarcinoma Cell Line |
| title_short | Study on Anti-cancer Effect of Plant Extract ACA-1 on Gastric Adenocarcinoma Cell Line |
| title_sort | study on anti cancer effect of plant extract aca 1 on gastric adenocarcinoma cell line |
| topic | cancer aca-1 apoptosis caspase |
| url | http://jmp.ir/article-1-172-en.html |
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