The spatiotemporal organization of cerebellar network activity resolved by two-photon imaging of multiple single neurons
In order to investigate the spatiotemporal organization of neuronal activity in local microcircuits, techniques allowing the simultaneous recording from multiple single neurons are required. To this end, we implemented an advanced spatial-light modulator two-photon microscope (SLM-2PM). A critical i...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-04-01
|
| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00092/full |
| _version_ | 1828412458303225856 |
|---|---|
| author | Daniela eGandolfi Daniela eGandolfi Paolo ePozzi Marialuisa eTognolina Giuseppe eChirico Jonathan eMapelli Jonathan eMapelli Egidio eD‘Angelo Egidio eD‘Angelo |
| author_facet | Daniela eGandolfi Daniela eGandolfi Paolo ePozzi Marialuisa eTognolina Giuseppe eChirico Jonathan eMapelli Jonathan eMapelli Egidio eD‘Angelo Egidio eD‘Angelo |
| author_sort | Daniela eGandolfi |
| collection | DOAJ |
| description | In order to investigate the spatiotemporal organization of neuronal activity in local microcircuits, techniques allowing the simultaneous recording from multiple single neurons are required. To this end, we implemented an advanced spatial-light modulator two-photon microscope (SLM-2PM). A critical issue for cerebellar theory is the organization of granular layer activity in the cerebellum, which has been predicted by single-cell recordings and computational models. With SLM-2PM, calcium signals could be recorded from different network elements in acute cerebellar slices including granule cells (GrCs), Purkinje cells (PCs)and molecular layer interneurons. By combining WCRs with SLM-2PM, the spike/calcium relationship in GrCs and PCs could be extrapolated toward the detection of single spikes. The SLM-2PM technique made it possible to monitor activity of over tens to hundreds neurons simultaneously. GrC activity depended on the number of spikes in the input mossy fiber bursts. PC and molecular layer interneuron activity paralleled that in the underlying GrC population revealing the spread of activity through the cerebellar cortical network. Moreover, circuit activity was increased by the GABA-A receptor blocker, gabazine, and reduced by the AMPA and NMDA receptor blockers, NBQX and APV. The SLM-2PM analysis of spatiotemporal patterns lent experimental support to the time-window and center-surround organizing principles of the granular layer. |
| first_indexed | 2024-12-10T12:49:28Z |
| format | Article |
| id | doaj.art-d7dcf977b32b4cee9b201b311af06f22 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-10T12:49:28Z |
| publishDate | 2014-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-d7dcf977b32b4cee9b201b311af06f222022-12-22T01:48:19ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022014-04-01810.3389/fncel.2014.0009277842The spatiotemporal organization of cerebellar network activity resolved by two-photon imaging of multiple single neuronsDaniela eGandolfi0Daniela eGandolfi1Paolo ePozzi2Marialuisa eTognolina3Giuseppe eChirico4Jonathan eMapelli5Jonathan eMapelli6Egidio eD‘Angelo7Egidio eD‘Angelo8University of PaviaUniversity of Modena and Reggio EmiliaUniversity of Milano-BicoccaUniversity of PaviaUniversity of Milano-BicoccaUniversity of Modena and Reggio EmiliaFondazione IRCCS C. MondinoUniversity of PaviaFondazione IRCCS C. MondinoIn order to investigate the spatiotemporal organization of neuronal activity in local microcircuits, techniques allowing the simultaneous recording from multiple single neurons are required. To this end, we implemented an advanced spatial-light modulator two-photon microscope (SLM-2PM). A critical issue for cerebellar theory is the organization of granular layer activity in the cerebellum, which has been predicted by single-cell recordings and computational models. With SLM-2PM, calcium signals could be recorded from different network elements in acute cerebellar slices including granule cells (GrCs), Purkinje cells (PCs)and molecular layer interneurons. By combining WCRs with SLM-2PM, the spike/calcium relationship in GrCs and PCs could be extrapolated toward the detection of single spikes. The SLM-2PM technique made it possible to monitor activity of over tens to hundreds neurons simultaneously. GrC activity depended on the number of spikes in the input mossy fiber bursts. PC and molecular layer interneuron activity paralleled that in the underlying GrC population revealing the spread of activity through the cerebellar cortical network. Moreover, circuit activity was increased by the GABA-A receptor blocker, gabazine, and reduced by the AMPA and NMDA receptor blockers, NBQX and APV. The SLM-2PM analysis of spatiotemporal patterns lent experimental support to the time-window and center-surround organizing principles of the granular layer.http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00092/fullCerebellumPurkinje Cellsmicrocircuitgranule cellstwo-photon microscopy |
| spellingShingle | Daniela eGandolfi Daniela eGandolfi Paolo ePozzi Marialuisa eTognolina Giuseppe eChirico Jonathan eMapelli Jonathan eMapelli Egidio eD‘Angelo Egidio eD‘Angelo The spatiotemporal organization of cerebellar network activity resolved by two-photon imaging of multiple single neurons Frontiers in Cellular Neuroscience Cerebellum Purkinje Cells microcircuit granule cells two-photon microscopy |
| title | The spatiotemporal organization of cerebellar network activity resolved by two-photon imaging of multiple single neurons |
| title_full | The spatiotemporal organization of cerebellar network activity resolved by two-photon imaging of multiple single neurons |
| title_fullStr | The spatiotemporal organization of cerebellar network activity resolved by two-photon imaging of multiple single neurons |
| title_full_unstemmed | The spatiotemporal organization of cerebellar network activity resolved by two-photon imaging of multiple single neurons |
| title_short | The spatiotemporal organization of cerebellar network activity resolved by two-photon imaging of multiple single neurons |
| title_sort | spatiotemporal organization of cerebellar network activity resolved by two photon imaging of multiple single neurons |
| topic | Cerebellum Purkinje Cells microcircuit granule cells two-photon microscopy |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00092/full |
| work_keys_str_mv | AT danielaegandolfi thespatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT danielaegandolfi thespatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT paoloepozzi thespatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT marialuisaetognolina thespatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT giuseppeechirico thespatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT jonathanemapelli thespatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT jonathanemapelli thespatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT egidioedangelo thespatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT egidioedangelo thespatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT danielaegandolfi spatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT danielaegandolfi spatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT paoloepozzi spatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT marialuisaetognolina spatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT giuseppeechirico spatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT jonathanemapelli spatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT jonathanemapelli spatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT egidioedangelo spatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons AT egidioedangelo spatiotemporalorganizationofcerebellarnetworkactivityresolvedbytwophotonimagingofmultiplesingleneurons |