Chemotactic behavior of egg mitochondria in response to sperm fusion in mice
Mitochondria are the powerhouses of eukaryotic cells and their positioning contributes to fertilization and early developmental processes. We report that sperm fusion triggers Ca2+ oscillations and mitochondrial movement toward fused sperm (mitochondrial chemotaxis) in mouse eggs. Mitochondria funct...
Main Authors: | , , , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
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Elsevier
2018-11-01
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Series: | Heliyon |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844018304304 |
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author | Maki Iwai Yuichirou Harada Rinako Miyabayashi Woojin Kang Akihiro Nakamura Natsuko Kawano Yoshitaka Miyamoto Mitsutoshi Yamada Toshio Hamatani Mami Miyado Keiichi Yoshida Hidekazu Saito Mamoru Tanaka Akihiro Umezawa Kenji Miyado |
author_facet | Maki Iwai Yuichirou Harada Rinako Miyabayashi Woojin Kang Akihiro Nakamura Natsuko Kawano Yoshitaka Miyamoto Mitsutoshi Yamada Toshio Hamatani Mami Miyado Keiichi Yoshida Hidekazu Saito Mamoru Tanaka Akihiro Umezawa Kenji Miyado |
author_sort | Maki Iwai |
collection | DOAJ |
description | Mitochondria are the powerhouses of eukaryotic cells and their positioning contributes to fertilization and early developmental processes. We report that sperm fusion triggers Ca2+ oscillations and mitochondrial movement toward fused sperm (mitochondrial chemotaxis) in mouse eggs. Mitochondria functioned in Ca2+ storage and were colocalized with endoplasmic reticulum (ER) during Ca2+ oscillations. Mitochondria then moved toward the fused sperm. Sperm extracts lacking nuclei induced Ca2+ oscillations, but did not promote mitochondrial chemotaxis. Our results suggest that sperm fusion motivates Ca2+ oscillation-independent mitochondrial chemotaxis. This phenomenon indicates that egg mitochondria interact with sperm materials, presumably nuclear substances, and their network tethers egg and sperm nuclei at the early stage of zygote formation. |
first_indexed | 2024-12-18T10:20:37Z |
format | Article |
id | doaj.art-d7e0604cc1f345c0a65740e5133163d1 |
institution | Directory Open Access Journal |
issn | 2405-8440 |
language | English |
last_indexed | 2024-12-18T10:20:37Z |
publishDate | 2018-11-01 |
publisher | Elsevier |
record_format | Article |
series | Heliyon |
spelling | doaj.art-d7e0604cc1f345c0a65740e5133163d12022-12-21T21:11:10ZengElsevierHeliyon2405-84402018-11-01411e00944Chemotactic behavior of egg mitochondria in response to sperm fusion in miceMaki Iwai0Yuichirou Harada1Rinako Miyabayashi2Woojin Kang3Akihiro Nakamura4Natsuko Kawano5Yoshitaka Miyamoto6Mitsutoshi Yamada7Toshio Hamatani8Mami Miyado9Keiichi Yoshida10Hidekazu Saito11Mamoru Tanaka12Akihiro Umezawa13Kenji Miyado14Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan; Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, JapanDepartment of Molecular Pathology, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku, Tokyo 192-0397, Japan; Corresponding author.Shinbashi YUME Clinic, 2-5-1 Shinbashi, Miyato, Tokyo 105-0004, JapanDepartment of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan; Department of Perinatal Medicine and Oocyte Care, National Center for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, JapanDepartment of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan; Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, JapanDepartment of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan; Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama, Kawasaki, Kanagawa 214-8571, JapanDepartment of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, JapanDepartment of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, JapanDepartment of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, JapanDepartment of Molecular Endocrinology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, JapanAdvanced Medicine, Innovation and Clinical Research Center, Tottori University Hospital, 36-1 Nishicho, Yonago, Tottori 683-8504, JapanDepartment of Perinatal Medicine and Oocyte Care, National Center for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, JapanDepartment of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, JapanDepartment of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, JapanDepartment of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan; Corresponding author.Mitochondria are the powerhouses of eukaryotic cells and their positioning contributes to fertilization and early developmental processes. We report that sperm fusion triggers Ca2+ oscillations and mitochondrial movement toward fused sperm (mitochondrial chemotaxis) in mouse eggs. Mitochondria functioned in Ca2+ storage and were colocalized with endoplasmic reticulum (ER) during Ca2+ oscillations. Mitochondria then moved toward the fused sperm. Sperm extracts lacking nuclei induced Ca2+ oscillations, but did not promote mitochondrial chemotaxis. Our results suggest that sperm fusion motivates Ca2+ oscillation-independent mitochondrial chemotaxis. This phenomenon indicates that egg mitochondria interact with sperm materials, presumably nuclear substances, and their network tethers egg and sperm nuclei at the early stage of zygote formation.http://www.sciencedirect.com/science/article/pii/S2405844018304304Cell biologyDevelopmental biologyMolecular biologyZoology |
spellingShingle | Maki Iwai Yuichirou Harada Rinako Miyabayashi Woojin Kang Akihiro Nakamura Natsuko Kawano Yoshitaka Miyamoto Mitsutoshi Yamada Toshio Hamatani Mami Miyado Keiichi Yoshida Hidekazu Saito Mamoru Tanaka Akihiro Umezawa Kenji Miyado Chemotactic behavior of egg mitochondria in response to sperm fusion in mice Heliyon Cell biology Developmental biology Molecular biology Zoology |
title | Chemotactic behavior of egg mitochondria in response to sperm fusion in mice |
title_full | Chemotactic behavior of egg mitochondria in response to sperm fusion in mice |
title_fullStr | Chemotactic behavior of egg mitochondria in response to sperm fusion in mice |
title_full_unstemmed | Chemotactic behavior of egg mitochondria in response to sperm fusion in mice |
title_short | Chemotactic behavior of egg mitochondria in response to sperm fusion in mice |
title_sort | chemotactic behavior of egg mitochondria in response to sperm fusion in mice |
topic | Cell biology Developmental biology Molecular biology Zoology |
url | http://www.sciencedirect.com/science/article/pii/S2405844018304304 |
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