The impact of pelvic floor electrical stimulation on vaginal microbiota and immunity
Pelvic floor electrical stimulation (ES) is an effective treatment for pelvic floor dysfunction. However, the impact of ES on vaginal microbiota and local inflammatory response is yet poorly understood. Therefore, we designed a longitudinal study to investigate the impact of ES on vaginal microbiota...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1006576/full |
| _version_ | 1818033081447088128 |
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| author | Yakun Zhang He Yang Chi Zhang Li Lin Wenlan Yang Guangwu Xiong Guolan Gao Guolan Gao |
| author_facet | Yakun Zhang He Yang Chi Zhang Li Lin Wenlan Yang Guangwu Xiong Guolan Gao Guolan Gao |
| author_sort | Yakun Zhang |
| collection | DOAJ |
| description | Pelvic floor electrical stimulation (ES) is an effective treatment for pelvic floor dysfunction. However, the impact of ES on vaginal microbiota and local inflammatory response is yet poorly understood. Therefore, we designed a longitudinal study to investigate the impact of ES on vaginal microbiota and cytokines. A total of 170 participants were recruited into the study at Peking University International Hospital, Beijing, China, from December 2021 to April 2022. They were divided into two groups concerning the follow-up: long-term cohort (n = 147) following up to seven treatment sessions and short-term cohort (n = 23) following up to 7 h after a 30-min treatment. Paired vaginal discharge samples were collected from 134 individuals. Vaginal microbiota was characterized by 16S rRNA sequencing, and local cytokines concentrations were detected by the cytometric bead array method. A significant increase in the relative abundance of Lactobacillus spp. was observed after ES treatment (P < 0.001). In addition, L. crispatus (P = 0.012) and L. gasseri (P = 0.011) also increased significantly. Reduced microbial diversity was observed in the vaginal microbiota after the treatment. In the long-term cohort, a significant downregulation of IFN-γ, IL-2, IL-4, IL-10, IL-17A, and TNF-α was compared with baseline. However, the short-term cohort presented with an elevated IL-6 level at 7 h after the treatment. In conclusion, this study suggested that transvaginal electrical stimulation might help to restore and maintain a healthy vaginal microbiota dominated by Lactobacillus, reducing the risk of vaginal inflammation. |
| first_indexed | 2024-12-10T06:17:36Z |
| format | Article |
| id | doaj.art-d7e06a17aa8b4162971757c6938208f2 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-10T06:17:36Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-d7e06a17aa8b4162971757c6938208f22022-12-22T01:59:24ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-09-011210.3389/fcimb.2022.10065761006576The impact of pelvic floor electrical stimulation on vaginal microbiota and immunityYakun Zhang0He Yang1Chi Zhang2Li Lin3Wenlan Yang4Guangwu Xiong5Guolan Gao6Guolan Gao7Savaid Medical School, University of Chinese Academy of Sciences, Beijing, ChinaSavaid Medical School, University of Chinese Academy of Sciences, Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University International Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University International Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University International Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University International Hospital, Beijing, ChinaSavaid Medical School, University of Chinese Academy of Sciences, Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University International Hospital, Beijing, ChinaPelvic floor electrical stimulation (ES) is an effective treatment for pelvic floor dysfunction. However, the impact of ES on vaginal microbiota and local inflammatory response is yet poorly understood. Therefore, we designed a longitudinal study to investigate the impact of ES on vaginal microbiota and cytokines. A total of 170 participants were recruited into the study at Peking University International Hospital, Beijing, China, from December 2021 to April 2022. They were divided into two groups concerning the follow-up: long-term cohort (n = 147) following up to seven treatment sessions and short-term cohort (n = 23) following up to 7 h after a 30-min treatment. Paired vaginal discharge samples were collected from 134 individuals. Vaginal microbiota was characterized by 16S rRNA sequencing, and local cytokines concentrations were detected by the cytometric bead array method. A significant increase in the relative abundance of Lactobacillus spp. was observed after ES treatment (P < 0.001). In addition, L. crispatus (P = 0.012) and L. gasseri (P = 0.011) also increased significantly. Reduced microbial diversity was observed in the vaginal microbiota after the treatment. In the long-term cohort, a significant downregulation of IFN-γ, IL-2, IL-4, IL-10, IL-17A, and TNF-α was compared with baseline. However, the short-term cohort presented with an elevated IL-6 level at 7 h after the treatment. In conclusion, this study suggested that transvaginal electrical stimulation might help to restore and maintain a healthy vaginal microbiota dominated by Lactobacillus, reducing the risk of vaginal inflammation.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1006576/fullvaginal microbiotaelectrical stimulation16S rRNApelvic floor musclelocal cytokine |
| spellingShingle | Yakun Zhang He Yang Chi Zhang Li Lin Wenlan Yang Guangwu Xiong Guolan Gao Guolan Gao The impact of pelvic floor electrical stimulation on vaginal microbiota and immunity Frontiers in Cellular and Infection Microbiology vaginal microbiota electrical stimulation 16S rRNA pelvic floor muscle local cytokine |
| title | The impact of pelvic floor electrical stimulation on vaginal microbiota and immunity |
| title_full | The impact of pelvic floor electrical stimulation on vaginal microbiota and immunity |
| title_fullStr | The impact of pelvic floor electrical stimulation on vaginal microbiota and immunity |
| title_full_unstemmed | The impact of pelvic floor electrical stimulation on vaginal microbiota and immunity |
| title_short | The impact of pelvic floor electrical stimulation on vaginal microbiota and immunity |
| title_sort | impact of pelvic floor electrical stimulation on vaginal microbiota and immunity |
| topic | vaginal microbiota electrical stimulation 16S rRNA pelvic floor muscle local cytokine |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1006576/full |
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