<i>Artemisinin</i> Loaded Cerium-Doped Nanopowders Improved In Vitro the Biomineralization in Human Periodontal Ligament Cells
Background: A promising strategy to enhance bone regeneration is the use of bioactive materials doped with metallic ions with therapeutic effects and their combination with active substances and/or drugs. The aim of the present study was to investigate the osteogenic capacity of human periodontal li...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/2/655 |
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| author | Ioannis Tsamesidis Anna Theocharidou Anastasia Beketova Maria Bousnaki Iason Chatzimentor Georgia K. Pouroutzidou Dimitrios Gkiliopoulos Eleana Kontonasaki |
| author_facet | Ioannis Tsamesidis Anna Theocharidou Anastasia Beketova Maria Bousnaki Iason Chatzimentor Georgia K. Pouroutzidou Dimitrios Gkiliopoulos Eleana Kontonasaki |
| author_sort | Ioannis Tsamesidis |
| collection | DOAJ |
| description | Background: A promising strategy to enhance bone regeneration is the use of bioactive materials doped with metallic ions with therapeutic effects and their combination with active substances and/or drugs. The aim of the present study was to investigate the osteogenic capacity of human periodontal ligament cells (hPDLCs) in culture with artemisinin (ART)-loaded Ce-doped calcium silicate nanopowders (NPs); Methods: Mesoporous silica, calcium-doped and calcium/cerium-doped silicate NPs were synthesized via a surfactant-assisted cooperative self-assembly process. Human periodontal ligament cells (hPDLCs) were isolated and tested for their osteogenic differentiation in the presence of ART-loaded and unloaded NPs through alkaline phosphatase (ALP) activity and Alizarine red S staining, while their antioxidant capacity was also evaluated; Results: ART promoted further the osteogenic differentiation of hPDLCs in the presence of Ce-doped NPs. Higher amounts of Ce in the ART-loaded NPs inversely affected the mineral deposition process by the hPDLCs. ART and Ce in the NPs have a synergistic role controlling the redox status and reducing ROS production from the hPDLCs; Conclusions: By monitoring the Ce amount and ART concentration, mesoporous NPs with optimum properties can be developed towards bone tissue regeneration demonstrating also potential application in periodontal tissue regeneration strategies. |
| first_indexed | 2024-03-11T08:17:22Z |
| format | Article |
| id | doaj.art-d7e0b92554334cc9bc67e0b8bb9c9041 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T08:17:22Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-d7e0b92554334cc9bc67e0b8bb9c90412023-11-16T22:42:51ZengMDPI AGPharmaceutics1999-49232023-02-0115265510.3390/pharmaceutics15020655<i>Artemisinin</i> Loaded Cerium-Doped Nanopowders Improved In Vitro the Biomineralization in Human Periodontal Ligament CellsIoannis Tsamesidis0Anna Theocharidou1Anastasia Beketova2Maria Bousnaki3Iason Chatzimentor4Georgia K. Pouroutzidou5Dimitrios Gkiliopoulos6Eleana Kontonasaki7Department of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceLaboratory of Chemical and Environmental Technology, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceBackground: A promising strategy to enhance bone regeneration is the use of bioactive materials doped with metallic ions with therapeutic effects and their combination with active substances and/or drugs. The aim of the present study was to investigate the osteogenic capacity of human periodontal ligament cells (hPDLCs) in culture with artemisinin (ART)-loaded Ce-doped calcium silicate nanopowders (NPs); Methods: Mesoporous silica, calcium-doped and calcium/cerium-doped silicate NPs were synthesized via a surfactant-assisted cooperative self-assembly process. Human periodontal ligament cells (hPDLCs) were isolated and tested for their osteogenic differentiation in the presence of ART-loaded and unloaded NPs through alkaline phosphatase (ALP) activity and Alizarine red S staining, while their antioxidant capacity was also evaluated; Results: ART promoted further the osteogenic differentiation of hPDLCs in the presence of Ce-doped NPs. Higher amounts of Ce in the ART-loaded NPs inversely affected the mineral deposition process by the hPDLCs. ART and Ce in the NPs have a synergistic role controlling the redox status and reducing ROS production from the hPDLCs; Conclusions: By monitoring the Ce amount and ART concentration, mesoporous NPs with optimum properties can be developed towards bone tissue regeneration demonstrating also potential application in periodontal tissue regeneration strategies.https://www.mdpi.com/1999-4923/15/2/655artemisininbio mineralizationcerium nanopowdershuman periodontal ligament cellsbone regeneration |
| spellingShingle | Ioannis Tsamesidis Anna Theocharidou Anastasia Beketova Maria Bousnaki Iason Chatzimentor Georgia K. Pouroutzidou Dimitrios Gkiliopoulos Eleana Kontonasaki <i>Artemisinin</i> Loaded Cerium-Doped Nanopowders Improved In Vitro the Biomineralization in Human Periodontal Ligament Cells Pharmaceutics artemisinin bio mineralization cerium nanopowders human periodontal ligament cells bone regeneration |
| title | <i>Artemisinin</i> Loaded Cerium-Doped Nanopowders Improved In Vitro the Biomineralization in Human Periodontal Ligament Cells |
| title_full | <i>Artemisinin</i> Loaded Cerium-Doped Nanopowders Improved In Vitro the Biomineralization in Human Periodontal Ligament Cells |
| title_fullStr | <i>Artemisinin</i> Loaded Cerium-Doped Nanopowders Improved In Vitro the Biomineralization in Human Periodontal Ligament Cells |
| title_full_unstemmed | <i>Artemisinin</i> Loaded Cerium-Doped Nanopowders Improved In Vitro the Biomineralization in Human Periodontal Ligament Cells |
| title_short | <i>Artemisinin</i> Loaded Cerium-Doped Nanopowders Improved In Vitro the Biomineralization in Human Periodontal Ligament Cells |
| title_sort | i artemisinin i loaded cerium doped nanopowders improved in vitro the biomineralization in human periodontal ligament cells |
| topic | artemisinin bio mineralization cerium nanopowders human periodontal ligament cells bone regeneration |
| url | https://www.mdpi.com/1999-4923/15/2/655 |
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