Obp56h Modulates Mating Behavior in Drosophila melanogaster
Social interactions in insects are driven by conspecific chemical signals that are detected via olfactory and gustatory neurons. Odorant binding proteins (Obps) transport volatile odorants to chemosensory receptors, but their effects on behaviors remain poorly characterized. Here, we report that RNA...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2016-10-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.116.034595 |
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| author | John R. Shorter Lauren M. Dembeck Logan J. Everett Tatiana V. Morozova Gunjan H. Arya Lavanya Turlapati Genevieve E. St. Armour Coby Schal Trudy F. C. Mackay Robert R. H. Anholt |
| author_facet | John R. Shorter Lauren M. Dembeck Logan J. Everett Tatiana V. Morozova Gunjan H. Arya Lavanya Turlapati Genevieve E. St. Armour Coby Schal Trudy F. C. Mackay Robert R. H. Anholt |
| author_sort | John R. Shorter |
| collection | DOAJ |
| description | Social interactions in insects are driven by conspecific chemical signals that are detected via olfactory and gustatory neurons. Odorant binding proteins (Obps) transport volatile odorants to chemosensory receptors, but their effects on behaviors remain poorly characterized. Here, we report that RNAi knockdown of Obp56h gene expression in Drosophila melanogaster enhances mating behavior by reducing courtship latency. The change in mating behavior that results from inhibition of Obp56h expression is accompanied by significant alterations in cuticular hydrocarbon (CHC) composition, including reduction in 5-tricosene (5-T), an inhibitory sex pheromone produced by males that increases copulation latency during courtship. Whole genome RNA sequencing confirms that expression of Obp56h is virtually abolished in Drosophila heads. Inhibition of Obp56h expression also affects expression of other chemoreception genes, including upregulation of lush in both sexes and Obp83ef in females, and reduction in expression of Obp19b and Or19b in males. In addition, several genes associated with lipid metabolism, which underlies the production of cuticular hydrocarbons, show altered transcript abundances. Our data show that modulation of mating behavior through reduction of Obp56h is accompanied by altered cuticular hydrocarbon profiles and implicate 5-T as a possible ligand for Obp56h. |
| first_indexed | 2024-12-18T23:44:39Z |
| format | Article |
| id | doaj.art-d7e1a2b58a244b3abaf51910873ed188 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-18T23:44:39Z |
| publishDate | 2016-10-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-d7e1a2b58a244b3abaf51910873ed1882022-12-21T20:47:16ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362016-10-016103335334210.1534/g3.116.03459527Obp56h Modulates Mating Behavior in Drosophila melanogasterJohn R. ShorterLauren M. DembeckLogan J. EverettTatiana V. MorozovaGunjan H. AryaLavanya TurlapatiGenevieve E. St. ArmourCoby SchalTrudy F. C. MackayRobert R. H. AnholtSocial interactions in insects are driven by conspecific chemical signals that are detected via olfactory and gustatory neurons. Odorant binding proteins (Obps) transport volatile odorants to chemosensory receptors, but their effects on behaviors remain poorly characterized. Here, we report that RNAi knockdown of Obp56h gene expression in Drosophila melanogaster enhances mating behavior by reducing courtship latency. The change in mating behavior that results from inhibition of Obp56h expression is accompanied by significant alterations in cuticular hydrocarbon (CHC) composition, including reduction in 5-tricosene (5-T), an inhibitory sex pheromone produced by males that increases copulation latency during courtship. Whole genome RNA sequencing confirms that expression of Obp56h is virtually abolished in Drosophila heads. Inhibition of Obp56h expression also affects expression of other chemoreception genes, including upregulation of lush in both sexes and Obp83ef in females, and reduction in expression of Obp19b and Or19b in males. In addition, several genes associated with lipid metabolism, which underlies the production of cuticular hydrocarbons, show altered transcript abundances. Our data show that modulation of mating behavior through reduction of Obp56h is accompanied by altered cuticular hydrocarbon profiles and implicate 5-T as a possible ligand for Obp56h.http://g3journal.org/lookup/doi/10.1534/g3.116.034595odorant binding proteinolfactioncuticular hydrocarbonpheromone5-tricoseneFlyBook |
| spellingShingle | John R. Shorter Lauren M. Dembeck Logan J. Everett Tatiana V. Morozova Gunjan H. Arya Lavanya Turlapati Genevieve E. St. Armour Coby Schal Trudy F. C. Mackay Robert R. H. Anholt Obp56h Modulates Mating Behavior in Drosophila melanogaster G3: Genes, Genomes, Genetics odorant binding protein olfaction cuticular hydrocarbon pheromone 5-tricosene FlyBook |
| title | Obp56h Modulates Mating Behavior in Drosophila melanogaster |
| title_full | Obp56h Modulates Mating Behavior in Drosophila melanogaster |
| title_fullStr | Obp56h Modulates Mating Behavior in Drosophila melanogaster |
| title_full_unstemmed | Obp56h Modulates Mating Behavior in Drosophila melanogaster |
| title_short | Obp56h Modulates Mating Behavior in Drosophila melanogaster |
| title_sort | obp56h modulates mating behavior in drosophila melanogaster |
| topic | odorant binding protein olfaction cuticular hydrocarbon pheromone 5-tricosene FlyBook |
| url | http://g3journal.org/lookup/doi/10.1534/g3.116.034595 |
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