Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models
Background and Aim: Endometriosis affects the ovaries and causes a decrease in the oocyte quality during endometrial receptivity. During the development of ovarian follicles, paracrine communication occurs between granulosa cells and oocytes. This study was conducted to determine the effects of bone...
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| Format: | Article |
| Language: | English |
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Veterinary World
2021-07-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.14/July-2021/9.pdf |
| _version_ | 1824024194695823360 |
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| author | Sri Ratna Dwiningsih Soehartono Darmosoekarto Hendy Hendarto Erry Gumilar Dachlan Fedik Abdul Rantam Sunarjo Sunarjo I. W. Arsana Wiyasa Widjiati Widjiati |
| author_facet | Sri Ratna Dwiningsih Soehartono Darmosoekarto Hendy Hendarto Erry Gumilar Dachlan Fedik Abdul Rantam Sunarjo Sunarjo I. W. Arsana Wiyasa Widjiati Widjiati |
| author_sort | Sri Ratna Dwiningsih |
| collection | DOAJ |
| description | Background and Aim: Endometriosis affects the ovaries and causes a decrease in the oocyte quality during endometrial receptivity. During the development of ovarian follicles, paracrine communication occurs between granulosa cells and oocytes. This study was conducted to determine the effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha (TNF-α) receptor 1 (TNFR1) expression, granulosa cell apoptosis, and folliculogenesis in endometriosis mouse models.
Materials and Methods: This study involved 42 female mice, which were divided into three groups: Healthy mice (T0), endometriosis mice without transplantation (T1), and endometriosis mice with bone marrow mesenchymal stem cell transplantation (T2). The mice were injected intraperitoneally with endometrial fragments (200 μL) to become endometriosis models. On day 15, the endometriosis models received mesenchymal stem cells. Sample collection was performed on day 29. Granulosa cell apoptosis and TNFR1 expression were examined using immunohistochemical staining, and folliculogenesis was assessed using hematoxylin and eosin staining of ovary samples. The data obtained from both examinations were statistically analyzed using Statistical Package for the Social Sciences.
Results: The results showed that TNFR1 expression is significantly decreased in T2 (p<0.004). The apoptosis of granulosa cells was lower in T2 (p<0.000). The primary, secondary, and graafian follicle counts in T2 were significantly increased.
Conclusion: Bone marrow mesenchymal stem cell transplantation in endometriosis mouse models can reduce TNFR1 expression and granulosa cell apoptosis and improve folliculogenesis. |
| first_indexed | 2024-12-19T09:44:56Z |
| format | Article |
| id | doaj.art-d7f084c98c7a41fdad4bf43e35c622a4 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-19T09:44:56Z |
| publishDate | 2021-07-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-d7f084c98c7a41fdad4bf43e35c622a42022-12-21T20:27:14ZengVeterinary WorldVeterinary World0972-89882231-09162021-07-011471788179610.14202/vetworld.2021.1788-1796Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse modelsSri Ratna Dwiningsih0https://orcid.org/0000-0002-3952-2020Soehartono Darmosoekarto1Hendy Hendarto2https://orcid.org/0000-0001-5551-2028Erry Gumilar Dachlan3https://orcid.org/0000-0002-1194-0597Fedik Abdul Rantam4https://orcid.org/0000-0002-1047-2410Sunarjo Sunarjo5I. W. Arsana Wiyasa6https://orcid.org/0000-0002-4922-588XWidjiati Widjiati7https://orcid.org/0000-0002-8376-1176Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Brawijaya Malang, Jl. Mayjen Prof. Dr. Moestopo No. 47, Surabaya 60132, Indonesia.Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.Background and Aim: Endometriosis affects the ovaries and causes a decrease in the oocyte quality during endometrial receptivity. During the development of ovarian follicles, paracrine communication occurs between granulosa cells and oocytes. This study was conducted to determine the effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha (TNF-α) receptor 1 (TNFR1) expression, granulosa cell apoptosis, and folliculogenesis in endometriosis mouse models. Materials and Methods: This study involved 42 female mice, which were divided into three groups: Healthy mice (T0), endometriosis mice without transplantation (T1), and endometriosis mice with bone marrow mesenchymal stem cell transplantation (T2). The mice were injected intraperitoneally with endometrial fragments (200 μL) to become endometriosis models. On day 15, the endometriosis models received mesenchymal stem cells. Sample collection was performed on day 29. Granulosa cell apoptosis and TNFR1 expression were examined using immunohistochemical staining, and folliculogenesis was assessed using hematoxylin and eosin staining of ovary samples. The data obtained from both examinations were statistically analyzed using Statistical Package for the Social Sciences. Results: The results showed that TNFR1 expression is significantly decreased in T2 (p<0.004). The apoptosis of granulosa cells was lower in T2 (p<0.000). The primary, secondary, and graafian follicle counts in T2 were significantly increased. Conclusion: Bone marrow mesenchymal stem cell transplantation in endometriosis mouse models can reduce TNFR1 expression and granulosa cell apoptosis and improve folliculogenesis.http://www.veterinaryworld.org/Vol.14/July-2021/9.pdfapoptosisbone marrow mesenchymal stem cellsendometriosisfolliculogenesisgranulosa cellstumor necrosis factor-alpha receptor 1 |
| spellingShingle | Sri Ratna Dwiningsih Soehartono Darmosoekarto Hendy Hendarto Erry Gumilar Dachlan Fedik Abdul Rantam Sunarjo Sunarjo I. W. Arsana Wiyasa Widjiati Widjiati Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models Veterinary World apoptosis bone marrow mesenchymal stem cells endometriosis folliculogenesis granulosa cells tumor necrosis factor-alpha receptor 1 |
| title | Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models |
| title_full | Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models |
| title_fullStr | Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models |
| title_full_unstemmed | Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models |
| title_short | Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models |
| title_sort | effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor alpha receptor 1 expression granulosa cell apoptosis and folliculogenesis repair in endometriosis mouse models |
| topic | apoptosis bone marrow mesenchymal stem cells endometriosis folliculogenesis granulosa cells tumor necrosis factor-alpha receptor 1 |
| url | http://www.veterinaryworld.org/Vol.14/July-2021/9.pdf |
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