Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model
Intervertebral disc (IVD) degeneration is a major cause of low back pain. However, treatments directly approaching the etiology of IVD degeneration and discogenic pain are not yet established. We previously demonstrated that intradiscal implantation of cell-free bioresorbable ultra-purified alginate...
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MDPI AG
2023-02-01
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| author | Hisataka Suzuki Katsuro Ura Daisuke Ukeba Takashi Suyama Norimasa Iwasaki Masatoki Watanabe Yumi Matsuzaki Katsuhisa Yamada Hideki Sudo |
| author_facet | Hisataka Suzuki Katsuro Ura Daisuke Ukeba Takashi Suyama Norimasa Iwasaki Masatoki Watanabe Yumi Matsuzaki Katsuhisa Yamada Hideki Sudo |
| author_sort | Hisataka Suzuki |
| collection | DOAJ |
| description | Intervertebral disc (IVD) degeneration is a major cause of low back pain. However, treatments directly approaching the etiology of IVD degeneration and discogenic pain are not yet established. We previously demonstrated that intradiscal implantation of cell-free bioresorbable ultra-purified alginate (UPAL) gel promotes tissue repair and reduces discogenic pain, and a combination of ultra-purified, Good Manufacturing Practice (GMP)-compliant, human bone marrow mesenchymal stem cells (rapidly expanding clones; RECs), and the UPAL gel increasingly enhanced IVD regeneration in animal models. This study investigated the therapeutic efficacy of injecting a mixture of REC and UPAL non-gelling solution for discogenic pain and IVD regeneration in a rat caudal nucleus pulposus punch model. REC and UPAL mixture and UPAL alone suppressed not only the expression of TNF-α, IL-6, and TrkA (<i>p</i> < 0.01, respectively), but also IVD degeneration and nociceptive behavior compared to punching alone (<i>p</i> < 0.01, respectively). Furthermore, REC and UPAL mixture suppressed these expression levels and nociceptive behavior compared to UPAL alone (<i>p</i> < 0.01, respectively). These results suggest that this minimally invasive treatment strategy with a single injection may be applied to treat discogenic pain and as a regenerative therapy. |
| first_indexed | 2024-03-11T09:49:32Z |
| format | Article |
| id | doaj.art-d7f14035cc0643a9babff6749df55fd7 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T09:49:32Z |
| publishDate | 2023-02-01 |
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| record_format | Article |
| series | Cells |
| spelling | doaj.art-d7f14035cc0643a9babff6749df55fd72023-11-16T16:22:36ZengMDPI AGCells2073-44092023-02-0112350510.3390/cells12030505Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat ModelHisataka Suzuki0Katsuro Ura1Daisuke Ukeba2Takashi Suyama3Norimasa Iwasaki4Masatoki Watanabe5Yumi Matsuzaki6Katsuhisa Yamada7Hideki Sudo8Department of Orthopedic Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, N15W7, Sapporo 060-8638, JapanDepartment of Orthopedic Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, N15W7, Sapporo 060-8638, JapanDepartment of Orthopedic Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, N15W7, Sapporo 060-8638, JapanPuREC/Bio-Venture, Shimane University, Izumo 693-8501, JapanDepartment of Orthopedic Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, N15W7, Sapporo 060-8638, JapanJapan Tissue Engineering Co., Ltd. (J-TEC), Gamagori 443-0022, JapanPuREC/Bio-Venture, Shimane University, Izumo 693-8501, JapanDepartment of Orthopedic Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, N15W7, Sapporo 060-8638, JapanDepartment of Orthopedic Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, N15W7, Sapporo 060-8638, JapanIntervertebral disc (IVD) degeneration is a major cause of low back pain. However, treatments directly approaching the etiology of IVD degeneration and discogenic pain are not yet established. We previously demonstrated that intradiscal implantation of cell-free bioresorbable ultra-purified alginate (UPAL) gel promotes tissue repair and reduces discogenic pain, and a combination of ultra-purified, Good Manufacturing Practice (GMP)-compliant, human bone marrow mesenchymal stem cells (rapidly expanding clones; RECs), and the UPAL gel increasingly enhanced IVD regeneration in animal models. This study investigated the therapeutic efficacy of injecting a mixture of REC and UPAL non-gelling solution for discogenic pain and IVD regeneration in a rat caudal nucleus pulposus punch model. REC and UPAL mixture and UPAL alone suppressed not only the expression of TNF-α, IL-6, and TrkA (<i>p</i> < 0.01, respectively), but also IVD degeneration and nociceptive behavior compared to punching alone (<i>p</i> < 0.01, respectively). Furthermore, REC and UPAL mixture suppressed these expression levels and nociceptive behavior compared to UPAL alone (<i>p</i> < 0.01, respectively). These results suggest that this minimally invasive treatment strategy with a single injection may be applied to treat discogenic pain and as a regenerative therapy.https://www.mdpi.com/2073-4409/12/3/505low back painintervertebral disc regenerationultra-purified clonogenic bone marrow-derived mesenchymal stem cellultra-purified alginate |
| spellingShingle | Hisataka Suzuki Katsuro Ura Daisuke Ukeba Takashi Suyama Norimasa Iwasaki Masatoki Watanabe Yumi Matsuzaki Katsuhisa Yamada Hideki Sudo Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model Cells low back pain intervertebral disc regeneration ultra-purified clonogenic bone marrow-derived mesenchymal stem cell ultra-purified alginate |
| title | Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model |
| title_full | Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model |
| title_fullStr | Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model |
| title_full_unstemmed | Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model |
| title_short | Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model |
| title_sort | injection of ultra purified stem cells with sodium alginate reduces discogenic pain in a rat model |
| topic | low back pain intervertebral disc regeneration ultra-purified clonogenic bone marrow-derived mesenchymal stem cell ultra-purified alginate |
| url | https://www.mdpi.com/2073-4409/12/3/505 |
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