Synergistic Inhibitory Activity of Bacillomycin D, Surfactin and Nisin against Thermoascus crustaceus, Neosartorya hiratsukae and Bacillus subtilis, Responsible for Cardboard Spoilage
Corrugated cardboard boxes are one of the largest paper-based packaging forms used for shipping and handling of wide variety of products in different end-use industries due to low cost, low weight and recyclability. Due to its organic composition, they are highly susceptible to spoilage from heat-re...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2020-12-01
|
| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/synergistic-inhibitory-activity-of-bacillomycin-d-surfactin-and-nisin-against-thermoascus-crustaceus-neosartorya-hiratsukae-and-bacillus-subtilis-responsible-for-cardboard-spoilage/ |
| _version_ | 1818726470614253568 |
|---|---|
| author | Uttara Vairagkar Rutuja Ankulkar Zafar Ahmed Yasmin Mirza |
| author_facet | Uttara Vairagkar Rutuja Ankulkar Zafar Ahmed Yasmin Mirza |
| author_sort | Uttara Vairagkar |
| collection | DOAJ |
| description | Corrugated cardboard boxes are one of the largest paper-based packaging forms used for shipping and handling of wide variety of products in different end-use industries due to low cost, low weight and recyclability. Due to its organic composition, they are highly susceptible to spoilage from heat-resistant microbial spores, leading to economic losses and health risks. In this study, the efficacy of lipopeptides produced from Bacillus amyloliquefaciens MTCC 10456 against thermotolerant Thermoascus crustaceus, Neosartorya hiratsukae and Bacillus subtilis, isolated from spoiled cardboard boxes, was investigated. Lipopeptides were isolated by salt-precipitation of fermentation broth and activity-guided Reverse Phase-High Performance Liquid Chromatography (RP-HPLC). Inhibitory fractions consisted of bacillomycin D and surfactin, which were identified using liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS/MS) analysis. Mixture of lipopeptides with nisin (3:2 w/w) asserted significant synergistic effect on the tested pathogens which reduced the minimum inhibitory concentrations (MIC) values and increased their inhibition spectra. Preservative coating containing lipopeptides and nisin was applied on the corrugated cardboard surfaces by mixing with starch-based additive by spread-coating method. It demonstrated biopreservative efficacy against the targeted microorganisms at during the observational period of 180 days. Reduction in microbial count of 4 log cycles was observed in 20 days and showed controlled release of coated peptides which indicate its suitability for packaging purposes. Findings from this study suggests an effective and scalable strategy to prevent microbial spoilage thereby extending the storage period of cardboard boxes. |
| first_indexed | 2024-12-17T21:58:43Z |
| format | Article |
| id | doaj.art-d7f25fcff597492a91aece651cdc2620 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-17T21:58:43Z |
| publishDate | 2020-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-d7f25fcff597492a91aece651cdc26202022-12-21T21:31:03ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2020-12-011442555256710.22207/JPAM.14.4.31Synergistic Inhibitory Activity of Bacillomycin D, Surfactin and Nisin against Thermoascus crustaceus, Neosartorya hiratsukae and Bacillus subtilis, Responsible for Cardboard SpoilageUttara VairagkarRutuja AnkulkarZafar AhmedYasmin Mirzahttps://orcid.org/0000-0002-9783-839XCorrugated cardboard boxes are one of the largest paper-based packaging forms used for shipping and handling of wide variety of products in different end-use industries due to low cost, low weight and recyclability. Due to its organic composition, they are highly susceptible to spoilage from heat-resistant microbial spores, leading to economic losses and health risks. In this study, the efficacy of lipopeptides produced from Bacillus amyloliquefaciens MTCC 10456 against thermotolerant Thermoascus crustaceus, Neosartorya hiratsukae and Bacillus subtilis, isolated from spoiled cardboard boxes, was investigated. Lipopeptides were isolated by salt-precipitation of fermentation broth and activity-guided Reverse Phase-High Performance Liquid Chromatography (RP-HPLC). Inhibitory fractions consisted of bacillomycin D and surfactin, which were identified using liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS/MS) analysis. Mixture of lipopeptides with nisin (3:2 w/w) asserted significant synergistic effect on the tested pathogens which reduced the minimum inhibitory concentrations (MIC) values and increased their inhibition spectra. Preservative coating containing lipopeptides and nisin was applied on the corrugated cardboard surfaces by mixing with starch-based additive by spread-coating method. It demonstrated biopreservative efficacy against the targeted microorganisms at during the observational period of 180 days. Reduction in microbial count of 4 log cycles was observed in 20 days and showed controlled release of coated peptides which indicate its suitability for packaging purposes. Findings from this study suggests an effective and scalable strategy to prevent microbial spoilage thereby extending the storage period of cardboard boxes.https://microbiologyjournal.org/synergistic-inhibitory-activity-of-bacillomycin-d-surfactin-and-nisin-against-thermoascus-crustaceus-neosartorya-hiratsukae-and-bacillus-subtilis-responsible-for-cardboard-spoilage/bacillus amyloliquefaciensthermotolerant microorganismspreservative coatinglipopeptidescorrugated cardboard boxes |
| spellingShingle | Uttara Vairagkar Rutuja Ankulkar Zafar Ahmed Yasmin Mirza Synergistic Inhibitory Activity of Bacillomycin D, Surfactin and Nisin against Thermoascus crustaceus, Neosartorya hiratsukae and Bacillus subtilis, Responsible for Cardboard Spoilage Journal of Pure and Applied Microbiology bacillus amyloliquefaciens thermotolerant microorganisms preservative coating lipopeptides corrugated cardboard boxes |
| title | Synergistic Inhibitory Activity of Bacillomycin D, Surfactin and Nisin against Thermoascus crustaceus, Neosartorya hiratsukae and Bacillus subtilis, Responsible for Cardboard Spoilage |
| title_full | Synergistic Inhibitory Activity of Bacillomycin D, Surfactin and Nisin against Thermoascus crustaceus, Neosartorya hiratsukae and Bacillus subtilis, Responsible for Cardboard Spoilage |
| title_fullStr | Synergistic Inhibitory Activity of Bacillomycin D, Surfactin and Nisin against Thermoascus crustaceus, Neosartorya hiratsukae and Bacillus subtilis, Responsible for Cardboard Spoilage |
| title_full_unstemmed | Synergistic Inhibitory Activity of Bacillomycin D, Surfactin and Nisin against Thermoascus crustaceus, Neosartorya hiratsukae and Bacillus subtilis, Responsible for Cardboard Spoilage |
| title_short | Synergistic Inhibitory Activity of Bacillomycin D, Surfactin and Nisin against Thermoascus crustaceus, Neosartorya hiratsukae and Bacillus subtilis, Responsible for Cardboard Spoilage |
| title_sort | synergistic inhibitory activity of bacillomycin d surfactin and nisin against thermoascus crustaceus neosartorya hiratsukae and bacillus subtilis responsible for cardboard spoilage |
| topic | bacillus amyloliquefaciens thermotolerant microorganisms preservative coating lipopeptides corrugated cardboard boxes |
| url | https://microbiologyjournal.org/synergistic-inhibitory-activity-of-bacillomycin-d-surfactin-and-nisin-against-thermoascus-crustaceus-neosartorya-hiratsukae-and-bacillus-subtilis-responsible-for-cardboard-spoilage/ |
| work_keys_str_mv | AT uttaravairagkar synergisticinhibitoryactivityofbacillomycindsurfactinandnisinagainstthermoascuscrustaceusneosartoryahiratsukaeandbacillussubtilisresponsibleforcardboardspoilage AT rutujaankulkar synergisticinhibitoryactivityofbacillomycindsurfactinandnisinagainstthermoascuscrustaceusneosartoryahiratsukaeandbacillussubtilisresponsibleforcardboardspoilage AT zafarahmed synergisticinhibitoryactivityofbacillomycindsurfactinandnisinagainstthermoascuscrustaceusneosartoryahiratsukaeandbacillussubtilisresponsibleforcardboardspoilage AT yasminmirza synergisticinhibitoryactivityofbacillomycindsurfactinandnisinagainstthermoascuscrustaceusneosartoryahiratsukaeandbacillussubtilisresponsibleforcardboardspoilage |