Effects of Dietary Microcapsule Sustained-Release Sodium Butyrate on the Growth Performance, Immunity, and Gut Microbiota of Yellow Broilers
The beneficial effects of butyric acid in poultry production are well documented, while the relationship between sodium butyrate (SB) and microcapsule sustained-release sodium butyrate (MSSB), especially in yellow broilers, remains poorly investigated. This study was designed to elucidate the functi...
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/23/3598 |
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| author | Zhenglie Dai Xiuxi Wang Yulan Liu Jinsong Liu Shiping Xiao Caimei Yang Yifan Zhong |
| author_facet | Zhenglie Dai Xiuxi Wang Yulan Liu Jinsong Liu Shiping Xiao Caimei Yang Yifan Zhong |
| author_sort | Zhenglie Dai |
| collection | DOAJ |
| description | The beneficial effects of butyric acid in poultry production are well documented, while the relationship between sodium butyrate (SB) and microcapsule sustained-release sodium butyrate (MSSB), especially in yellow broilers, remains poorly investigated. This study was designed to elucidate the function as well as the potential mechanisms of SB and MSSB in enhancing health in yellow broilers. In total, 360 one-day-old yellow broilers were allocated to three treatment groups. The control group (CON) received a basic diet, while the SB group was provided with 1000 mg/kg of sodium butyrate (SB), and the MSSB received microcapsule sustained-release sodium butyrate (MSSB), all over a period of 56 days. Compared to the CON group, the dietary supplementation of both SB and MSSB showed a lower feed:gain ratio (<i>p</i> < 0.01). No significant (<i>p</i> > 0.05) difference in antioxidant capacity was observed between the three groups. We observed significantly higher levels (<i>p</i> < 0.05) of immunoglobulins and a reduction in concentrations in both the SB and MSSB groups compared to the CON group. Furthermore, both SB and MSSB induced alterations in the diversity, structure, and function of gut microbiota. MSSB demonstrated even more pronounced beneficial effects than SB, particularly in regard to the serum IgA level (<i>p</i> = 0.05), cecal isovalerate concentration (<i>p</i> < 0.05), and villus height (<i>p</i> < 0.01). The sequencing of the gut microbiota revealed that MSSB led to a significant increase in the relative abundance of <i>Clostridia</i> UCG-014, <i>Bacilli</i> RF39, and <i>Oscillospiraceae</i> UCG-005. Predictions of bacterial function indicated changes in KEGG pathways, including an enrichment of tryptophan metabolism (ko00380), and a reduction in fructose and mannose metabolism (ko00051), chloroalkane and chloroalkene degradation (ko00625), and naphthalene degradation (ko00626) in yellow broilers fed with MSSB. Among these, the mediation analysis revealed a causal effect between the <i>Clostridia</i> UCG-014 in the gut and serum IgA, with tryptophan metabolism being a key mediator in this relationship. Our results suggest that dietary MSSB can improve the growth performance, immunity, and gut microbiota of yellow broilers. MSSB increased the abundance of <i>Clostridia</i> UCG-014 and activated the tryptophan metabolism pathway (ko00380), contributing to IgA levels in yellow broilers through this mechanism. |
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| spelling | doaj.art-d7f347e274d24000ab14bb7daa7baa692023-12-08T15:10:24ZengMDPI AGAnimals2076-26152023-11-011323359810.3390/ani13233598Effects of Dietary Microcapsule Sustained-Release Sodium Butyrate on the Growth Performance, Immunity, and Gut Microbiota of Yellow BroilersZhenglie Dai0Xiuxi Wang1Yulan Liu2Jinsong Liu3Shiping Xiao4Caimei Yang5Yifan Zhong6College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang Agricultural and Forestry University, Hangzhou 311300, ChinaCollege of Animal Science and Technology, College of Veterinary Medicine, Zhejiang Agricultural and Forestry University, Hangzhou 311300, ChinaZhejiang Vegamax Biotechnology Co., Ltd., Huzhou 313300, ChinaZhejiang Vegamax Biotechnology Co., Ltd., Huzhou 313300, ChinaZhejiang Vegamax Biotechnology Co., Ltd., Huzhou 313300, ChinaZhejiang Vegamax Biotechnology Co., Ltd., Huzhou 313300, ChinaCollege of Animal Science and Technology, College of Veterinary Medicine, Zhejiang Agricultural and Forestry University, Hangzhou 311300, ChinaThe beneficial effects of butyric acid in poultry production are well documented, while the relationship between sodium butyrate (SB) and microcapsule sustained-release sodium butyrate (MSSB), especially in yellow broilers, remains poorly investigated. This study was designed to elucidate the function as well as the potential mechanisms of SB and MSSB in enhancing health in yellow broilers. In total, 360 one-day-old yellow broilers were allocated to three treatment groups. The control group (CON) received a basic diet, while the SB group was provided with 1000 mg/kg of sodium butyrate (SB), and the MSSB received microcapsule sustained-release sodium butyrate (MSSB), all over a period of 56 days. Compared to the CON group, the dietary supplementation of both SB and MSSB showed a lower feed:gain ratio (<i>p</i> < 0.01). No significant (<i>p</i> > 0.05) difference in antioxidant capacity was observed between the three groups. We observed significantly higher levels (<i>p</i> < 0.05) of immunoglobulins and a reduction in concentrations in both the SB and MSSB groups compared to the CON group. Furthermore, both SB and MSSB induced alterations in the diversity, structure, and function of gut microbiota. MSSB demonstrated even more pronounced beneficial effects than SB, particularly in regard to the serum IgA level (<i>p</i> = 0.05), cecal isovalerate concentration (<i>p</i> < 0.05), and villus height (<i>p</i> < 0.01). The sequencing of the gut microbiota revealed that MSSB led to a significant increase in the relative abundance of <i>Clostridia</i> UCG-014, <i>Bacilli</i> RF39, and <i>Oscillospiraceae</i> UCG-005. Predictions of bacterial function indicated changes in KEGG pathways, including an enrichment of tryptophan metabolism (ko00380), and a reduction in fructose and mannose metabolism (ko00051), chloroalkane and chloroalkene degradation (ko00625), and naphthalene degradation (ko00626) in yellow broilers fed with MSSB. Among these, the mediation analysis revealed a causal effect between the <i>Clostridia</i> UCG-014 in the gut and serum IgA, with tryptophan metabolism being a key mediator in this relationship. Our results suggest that dietary MSSB can improve the growth performance, immunity, and gut microbiota of yellow broilers. MSSB increased the abundance of <i>Clostridia</i> UCG-014 and activated the tryptophan metabolism pathway (ko00380), contributing to IgA levels in yellow broilers through this mechanism.https://www.mdpi.com/2076-2615/13/23/3598microcapsule sustained-release sodium butyrateyellow broilergut healthimmunitygut microbiota |
| spellingShingle | Zhenglie Dai Xiuxi Wang Yulan Liu Jinsong Liu Shiping Xiao Caimei Yang Yifan Zhong Effects of Dietary Microcapsule Sustained-Release Sodium Butyrate on the Growth Performance, Immunity, and Gut Microbiota of Yellow Broilers Animals microcapsule sustained-release sodium butyrate yellow broiler gut health immunity gut microbiota |
| title | Effects of Dietary Microcapsule Sustained-Release Sodium Butyrate on the Growth Performance, Immunity, and Gut Microbiota of Yellow Broilers |
| title_full | Effects of Dietary Microcapsule Sustained-Release Sodium Butyrate on the Growth Performance, Immunity, and Gut Microbiota of Yellow Broilers |
| title_fullStr | Effects of Dietary Microcapsule Sustained-Release Sodium Butyrate on the Growth Performance, Immunity, and Gut Microbiota of Yellow Broilers |
| title_full_unstemmed | Effects of Dietary Microcapsule Sustained-Release Sodium Butyrate on the Growth Performance, Immunity, and Gut Microbiota of Yellow Broilers |
| title_short | Effects of Dietary Microcapsule Sustained-Release Sodium Butyrate on the Growth Performance, Immunity, and Gut Microbiota of Yellow Broilers |
| title_sort | effects of dietary microcapsule sustained release sodium butyrate on the growth performance immunity and gut microbiota of yellow broilers |
| topic | microcapsule sustained-release sodium butyrate yellow broiler gut health immunity gut microbiota |
| url | https://www.mdpi.com/2076-2615/13/23/3598 |
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