Advances in the differential molecular diagnosis of vesicular disease pathogens in swine
Foot-and-mouth disease virus (FMDV), Senecavirus A (SVA) and swine vesicular disease virus (SVDV) are members of the family Picornaviridae, which can cause similar symptoms - vesicular lesions in the tissues of the mouth, nose, feet, skin and mucous membrane of animals. Rapid and accurate diagnosis...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1019876/full |
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| author | Wenxian Chen Wenxian Chen Weijun Wang Weijun Wang Xinyan Wang Xinyan Wang Zhaoyao Li Zhaoyao Li Keke Wu Keke Wu Xiaowen Li Xiaowen Li Yuwan Li Yuwan Li Yuwan Li Lin Yi Lin Yi Lin Yi Mingqiu Zhao Mingqiu Zhao Mingqiu Zhao Hongxing Ding Hongxing Ding Hongxing Ding Shuangqi Fan Shuangqi Fan Jinding Chen Jinding Chen |
| author_facet | Wenxian Chen Wenxian Chen Weijun Wang Weijun Wang Xinyan Wang Xinyan Wang Zhaoyao Li Zhaoyao Li Keke Wu Keke Wu Xiaowen Li Xiaowen Li Yuwan Li Yuwan Li Yuwan Li Lin Yi Lin Yi Lin Yi Mingqiu Zhao Mingqiu Zhao Mingqiu Zhao Hongxing Ding Hongxing Ding Hongxing Ding Shuangqi Fan Shuangqi Fan Jinding Chen Jinding Chen |
| author_sort | Wenxian Chen |
| collection | DOAJ |
| description | Foot-and-mouth disease virus (FMDV), Senecavirus A (SVA) and swine vesicular disease virus (SVDV) are members of the family Picornaviridae, which can cause similar symptoms - vesicular lesions in the tissues of the mouth, nose, feet, skin and mucous membrane of animals. Rapid and accurate diagnosis of these viruses allows for control measures to prevent the spread of these diseases. Reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR are traditional and reliable methods for pathogen detection, while their amplification reaction requires a thermocycler. Isothermal amplification methods including loop-mediated isothermal amplification and recombinase polymerase amplification developed in recent years are simple, rapid and do not require specialized equipment, allowing for point of care diagnostics. Luminex technology allows for simultaneous detection of multiple pathogens. CRISPR-Cas diagnostic systems also emerging nucleic acid detection technologies which are very sensitivity and specificity. In this paper, various nucleic acid detection methods aimed at vesicular disease pathogens in swine (including FMDV, SVA and SVDV) are summarized. |
| first_indexed | 2024-04-12T11:46:32Z |
| format | Article |
| id | doaj.art-d7f4a6c81bfc4ae6a9bbe505b33e28b4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T11:46:32Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d7f4a6c81bfc4ae6a9bbe505b33e28b42022-12-22T03:34:21ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10198761019876Advances in the differential molecular diagnosis of vesicular disease pathogens in swineWenxian Chen0Wenxian Chen1Weijun Wang2Weijun Wang3Xinyan Wang4Xinyan Wang5Zhaoyao Li6Zhaoyao Li7Keke Wu8Keke Wu9Xiaowen Li10Xiaowen Li11Yuwan Li12Yuwan Li13Yuwan Li14Lin Yi15Lin Yi16Lin Yi17Mingqiu Zhao18Mingqiu Zhao19Mingqiu Zhao20Hongxing Ding21Hongxing Ding22Hongxing Ding23Shuangqi Fan24Shuangqi Fan25Jinding Chen26Jinding Chen27College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaFoot-and-mouth disease virus (FMDV), Senecavirus A (SVA) and swine vesicular disease virus (SVDV) are members of the family Picornaviridae, which can cause similar symptoms - vesicular lesions in the tissues of the mouth, nose, feet, skin and mucous membrane of animals. Rapid and accurate diagnosis of these viruses allows for control measures to prevent the spread of these diseases. Reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR are traditional and reliable methods for pathogen detection, while their amplification reaction requires a thermocycler. Isothermal amplification methods including loop-mediated isothermal amplification and recombinase polymerase amplification developed in recent years are simple, rapid and do not require specialized equipment, allowing for point of care diagnostics. Luminex technology allows for simultaneous detection of multiple pathogens. CRISPR-Cas diagnostic systems also emerging nucleic acid detection technologies which are very sensitivity and specificity. In this paper, various nucleic acid detection methods aimed at vesicular disease pathogens in swine (including FMDV, SVA and SVDV) are summarized.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1019876/fullFMDVSVASVDVPCRisothermal amplificationLuminex |
| spellingShingle | Wenxian Chen Wenxian Chen Weijun Wang Weijun Wang Xinyan Wang Xinyan Wang Zhaoyao Li Zhaoyao Li Keke Wu Keke Wu Xiaowen Li Xiaowen Li Yuwan Li Yuwan Li Yuwan Li Lin Yi Lin Yi Lin Yi Mingqiu Zhao Mingqiu Zhao Mingqiu Zhao Hongxing Ding Hongxing Ding Hongxing Ding Shuangqi Fan Shuangqi Fan Jinding Chen Jinding Chen Advances in the differential molecular diagnosis of vesicular disease pathogens in swine Frontiers in Microbiology FMDV SVA SVDV PCR isothermal amplification Luminex |
| title | Advances in the differential molecular diagnosis of vesicular disease pathogens in swine |
| title_full | Advances in the differential molecular diagnosis of vesicular disease pathogens in swine |
| title_fullStr | Advances in the differential molecular diagnosis of vesicular disease pathogens in swine |
| title_full_unstemmed | Advances in the differential molecular diagnosis of vesicular disease pathogens in swine |
| title_short | Advances in the differential molecular diagnosis of vesicular disease pathogens in swine |
| title_sort | advances in the differential molecular diagnosis of vesicular disease pathogens in swine |
| topic | FMDV SVA SVDV PCR isothermal amplification Luminex |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1019876/full |
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