Intergenerational effects of overfeeding on aversive learning in zebrafish (Danio rerio)
Abstract The obesity epidemic is concerning as obesity appears to negatively impact cognition and behavior. Furthermore, some studies suggest that this negative effect could be carried across generations from both mothers and fathers although evidence is not consistent. Here, we attempt to address h...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-10-01
|
| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.9423 |
| _version_ | 1797987373283278848 |
|---|---|
| author | Hamza Anwer Dominic Mason Susanne Zajitschek Daniel Hesselson Daniel W. A. Noble Margaret J. Morris Malgorzata Lagisz Shinichi Nakagawa |
| author_facet | Hamza Anwer Dominic Mason Susanne Zajitschek Daniel Hesselson Daniel W. A. Noble Margaret J. Morris Malgorzata Lagisz Shinichi Nakagawa |
| author_sort | Hamza Anwer |
| collection | DOAJ |
| description | Abstract The obesity epidemic is concerning as obesity appears to negatively impact cognition and behavior. Furthermore, some studies suggest that this negative effect could be carried across generations from both mothers and fathers although evidence is not consistent. Here, we attempt to address how obesogenic diets in the parental generation (F0) can impact offspring's cognition and anxiety intergenerationally (F1) in a zebrafish model. We compare both mean trait values and their variances. Using a multifactorial design, we created a total of four groups: F1T (treatment mothers × treatment fathers); F1M (treatment mothers × control fathers); F1P (treatment fathers × control mothers); and F1C (control mothers × control fathers, F1C); and subjected them to anxiety tank tests and aversive learning assays. When both parents were exposed, offspring (F1T) displayed the poorest aversive learning, while offspring that only had one parent exposed (F1P and F1M) learnt the aversive learning task the best. Zebrafish in all groups displayed no statistically significant differences in anxiety‐associated behaviors. Males and females also performed similarly in both anxiety and aversive learning assays. While all F1 groups had similar levels of fasting blood glucose, variance in glucose levels were reduced in F1P and F1T indicating the importance of investigating heteroskedasticity between groups. Furthermore, anxiety behaviors of these two groups appeared to be less repeatable. To our knowledge, this is the first study to test the intergenerational effects of an obesogenic diet on zebrafish cognition. Our multifactorial design as well as repeated tests also allowed us to disentangle maternal and paternal effects (as well as combined effects) and accurately detect subtle information such as between‐individual variation. |
| first_indexed | 2024-04-11T07:47:40Z |
| format | Article |
| id | doaj.art-d7f4ee21236a49f09f9a6b65cac8c341 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-04-11T07:47:40Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-d7f4ee21236a49f09f9a6b65cac8c3412022-12-22T04:36:14ZengWileyEcology and Evolution2045-77582022-10-011210n/an/a10.1002/ece3.9423Intergenerational effects of overfeeding on aversive learning in zebrafish (Danio rerio)Hamza Anwer0Dominic Mason1Susanne Zajitschek2Daniel Hesselson3Daniel W. A. Noble4Margaret J. Morris5Malgorzata Lagisz6Shinichi Nakagawa7Evolution & Ecology Research Centre and School of Biological, Earth and Environmental Sciences University of New South Wales Sydney New South Wales AustraliaEvolution & Ecology Research Centre and School of Biological, Earth and Environmental Sciences University of New South Wales Sydney New South Wales AustraliaEvolution & Ecology Research Centre and School of Biological, Earth and Environmental Sciences University of New South Wales Sydney New South Wales AustraliaDiabetes and Metabolism Division Garvan Institute of Medical Research Darlinghurst, Sydney New South Wales AustraliaDivision of Ecology and Evolution, Research School of Biology The Australian National University Canberra Australian Capital Territory AustraliaDepartment of Pharmacology, School of Medical Sciences University of New South Wales Sydney New South Wales AustraliaEvolution & Ecology Research Centre and School of Biological, Earth and Environmental Sciences University of New South Wales Sydney New South Wales AustraliaEvolution & Ecology Research Centre and School of Biological, Earth and Environmental Sciences University of New South Wales Sydney New South Wales AustraliaAbstract The obesity epidemic is concerning as obesity appears to negatively impact cognition and behavior. Furthermore, some studies suggest that this negative effect could be carried across generations from both mothers and fathers although evidence is not consistent. Here, we attempt to address how obesogenic diets in the parental generation (F0) can impact offspring's cognition and anxiety intergenerationally (F1) in a zebrafish model. We compare both mean trait values and their variances. Using a multifactorial design, we created a total of four groups: F1T (treatment mothers × treatment fathers); F1M (treatment mothers × control fathers); F1P (treatment fathers × control mothers); and F1C (control mothers × control fathers, F1C); and subjected them to anxiety tank tests and aversive learning assays. When both parents were exposed, offspring (F1T) displayed the poorest aversive learning, while offspring that only had one parent exposed (F1P and F1M) learnt the aversive learning task the best. Zebrafish in all groups displayed no statistically significant differences in anxiety‐associated behaviors. Males and females also performed similarly in both anxiety and aversive learning assays. While all F1 groups had similar levels of fasting blood glucose, variance in glucose levels were reduced in F1P and F1T indicating the importance of investigating heteroskedasticity between groups. Furthermore, anxiety behaviors of these two groups appeared to be less repeatable. To our knowledge, this is the first study to test the intergenerational effects of an obesogenic diet on zebrafish cognition. Our multifactorial design as well as repeated tests also allowed us to disentangle maternal and paternal effects (as well as combined effects) and accurately detect subtle information such as between‐individual variation.https://doi.org/10.1002/ece3.9423behaviorcognitionintergenerationalobesogenic dietrepeatabilityzebrafish |
| spellingShingle | Hamza Anwer Dominic Mason Susanne Zajitschek Daniel Hesselson Daniel W. A. Noble Margaret J. Morris Malgorzata Lagisz Shinichi Nakagawa Intergenerational effects of overfeeding on aversive learning in zebrafish (Danio rerio) Ecology and Evolution behavior cognition intergenerational obesogenic diet repeatability zebrafish |
| title | Intergenerational effects of overfeeding on aversive learning in zebrafish (Danio rerio) |
| title_full | Intergenerational effects of overfeeding on aversive learning in zebrafish (Danio rerio) |
| title_fullStr | Intergenerational effects of overfeeding on aversive learning in zebrafish (Danio rerio) |
| title_full_unstemmed | Intergenerational effects of overfeeding on aversive learning in zebrafish (Danio rerio) |
| title_short | Intergenerational effects of overfeeding on aversive learning in zebrafish (Danio rerio) |
| title_sort | intergenerational effects of overfeeding on aversive learning in zebrafish danio rerio |
| topic | behavior cognition intergenerational obesogenic diet repeatability zebrafish |
| url | https://doi.org/10.1002/ece3.9423 |
| work_keys_str_mv | AT hamzaanwer intergenerationaleffectsofoverfeedingonaversivelearninginzebrafishdaniorerio AT dominicmason intergenerationaleffectsofoverfeedingonaversivelearninginzebrafishdaniorerio AT susannezajitschek intergenerationaleffectsofoverfeedingonaversivelearninginzebrafishdaniorerio AT danielhesselson intergenerationaleffectsofoverfeedingonaversivelearninginzebrafishdaniorerio AT danielwanoble intergenerationaleffectsofoverfeedingonaversivelearninginzebrafishdaniorerio AT margaretjmorris intergenerationaleffectsofoverfeedingonaversivelearninginzebrafishdaniorerio AT malgorzatalagisz intergenerationaleffectsofoverfeedingonaversivelearninginzebrafishdaniorerio AT shinichinakagawa intergenerationaleffectsofoverfeedingonaversivelearninginzebrafishdaniorerio |