| Summary: | The golden twin-spot moth (<i>Chrysodeixis chalcites</i>) is one of the most important pests in banana production on the Canary Islands (Spain). The efficacy of different biorational insecticides based on bioenzyme complexes (Intruder<sup>®</sup>), plant extracts: Rutaceae and Piperaceae (Avenger<sup>®</sup>), Rutaceae and Lauraceae (BioKnock<sup>®</sup>), cinnamon, citronella, and Menta (Cinamite<sup>®</sup>), Alliaceae and Solanacea (Garlitrol-Forte<sup>®</sup>), citrus (Prevam<sup>®</sup>), and neem oil (Indasol<sup>®</sup>) was assessed against <i>C. chalcites</i>. Laboratory assays included: choice (repellent effect), no choice, and contact toxicity on <i>C. chalcites</i> 2nd instar larvae. The highest repellent effect was observed with Prevam<sup>®</sup> (85.19 ± 1.7%), followed by Garlitrol<sup>®</sup> (68.44 ± 5.7%) and Intruder<sup>®</sup> (67.54 ± 4.3%). In no choice assays, Prevam<sup>®</sup> (0.92 ± 0.4%), Indasol<sup>®</sup> (0.98 ± 0.33%), and Intruder<sup>®</sup> (2.7 ± 0.33%) had the lowest leaf consumption. The contact toxicity assays showed the highest mortality with Intruder<sup>®</sup> both at 1 day and 7 days post-application (20.22 ± 2.98% and 77.77 ± 5.7%, respectively). In the screenhouse trial, the best results for <i>C. chalcites</i> larvae mortality, fruit damage, and fruit classification in quality categories 7 days after application of the bioinsecticide were obtained with Intruder<sup>®</sup>, Prevam<sup>®</sup>, and Indasol<sup>®</sup>. An economic analysis of biorational treatments was also performed. The results of this study provide successful alternatives to chemical pesticides for the control of <i>C. chalcites</i> on banana plants in the Canary Islands.
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