A Novel H-2<sup>d</sup> Epitope for Influenza A Polymerase Acidic Protein
Understanding the complexity of the T-cell epitope hierarchy in humans through mouse models can be difficult. In particular, using only one murine strain, the C57BL/6 mouse, to investigate the immune response to influenza virus infection limits our understanding. In the present study, by immunizing...
| Principais autores: | , , , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
MDPI AG
2022-03-01
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| coleção: | Viruses |
| Assuntos: | |
| Acesso em linha: | https://www.mdpi.com/1999-4915/14/3/601 |
| _version_ | 1827624767016927232 |
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| author | Ida Uddbäck Jacob E. Kohlmeier Allan R. Thomsen Jan P. Christensen |
| author_facet | Ida Uddbäck Jacob E. Kohlmeier Allan R. Thomsen Jan P. Christensen |
| author_sort | Ida Uddbäck |
| collection | DOAJ |
| description | Understanding the complexity of the T-cell epitope hierarchy in humans through mouse models can be difficult. In particular, using only one murine strain, the C57BL/6 mouse, to investigate the immune response to influenza virus infection limits our understanding. In the present study, by immunizing C57BL/6 mice with an adenoviral vector encoding the polymerase acidic (AdIiPA) protein of influenza A virus, we were able to induce a high number of PA-specific T cells. However, upon challenge, these cells were only partly protective. When instead immunizing BALB/c mice with AdIiPA, we found that the immunized mice were fully protected against challenge. We found that this protection was dependent on CD8 T cells, and we identified a novel H-2D<sup>d</sup>-restricted epitope, PA33. These findings provide a new tool for researchers to study PA-specific immunity in mice with an H-2<sup>d</sup> haplotype. Additionally, our findings underscore the importance of critically evaluating important limitations of using a single inbred mouse strain in vaccine studies. |
| first_indexed | 2024-03-09T12:16:16Z |
| format | Article |
| id | doaj.art-d7f9f34b36ec4912a4d0236b885f5c7d |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:16:16Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-d7f9f34b36ec4912a4d0236b885f5c7d2023-11-30T22:46:55ZengMDPI AGViruses1999-49152022-03-0114360110.3390/v14030601A Novel H-2<sup>d</sup> Epitope for Influenza A Polymerase Acidic ProteinIda Uddbäck0Jacob E. Kohlmeier1Allan R. Thomsen2Jan P. Christensen3Department of Immunology and Microbiology, University of Copenhagen, DK-2200 Copenhagen, DenmarkDepartment of Microbiology and Immunology, Emory University, Atlanta, GA 30322, USADepartment of Immunology and Microbiology, University of Copenhagen, DK-2200 Copenhagen, DenmarkDepartment of Immunology and Microbiology, University of Copenhagen, DK-2200 Copenhagen, DenmarkUnderstanding the complexity of the T-cell epitope hierarchy in humans through mouse models can be difficult. In particular, using only one murine strain, the C57BL/6 mouse, to investigate the immune response to influenza virus infection limits our understanding. In the present study, by immunizing C57BL/6 mice with an adenoviral vector encoding the polymerase acidic (AdIiPA) protein of influenza A virus, we were able to induce a high number of PA-specific T cells. However, upon challenge, these cells were only partly protective. When instead immunizing BALB/c mice with AdIiPA, we found that the immunized mice were fully protected against challenge. We found that this protection was dependent on CD8 T cells, and we identified a novel H-2D<sup>d</sup>-restricted epitope, PA33. These findings provide a new tool for researchers to study PA-specific immunity in mice with an H-2<sup>d</sup> haplotype. Additionally, our findings underscore the importance of critically evaluating important limitations of using a single inbred mouse strain in vaccine studies.https://www.mdpi.com/1999-4915/14/3/601T cellT-cell immunitymucosal immunityadenovirusadenoviral vaccinesmurine models |
| spellingShingle | Ida Uddbäck Jacob E. Kohlmeier Allan R. Thomsen Jan P. Christensen A Novel H-2<sup>d</sup> Epitope for Influenza A Polymerase Acidic Protein Viruses T cell T-cell immunity mucosal immunity adenovirus adenoviral vaccines murine models |
| title | A Novel H-2<sup>d</sup> Epitope for Influenza A Polymerase Acidic Protein |
| title_full | A Novel H-2<sup>d</sup> Epitope for Influenza A Polymerase Acidic Protein |
| title_fullStr | A Novel H-2<sup>d</sup> Epitope for Influenza A Polymerase Acidic Protein |
| title_full_unstemmed | A Novel H-2<sup>d</sup> Epitope for Influenza A Polymerase Acidic Protein |
| title_short | A Novel H-2<sup>d</sup> Epitope for Influenza A Polymerase Acidic Protein |
| title_sort | novel h 2 sup d sup epitope for influenza a polymerase acidic protein |
| topic | T cell T-cell immunity mucosal immunity adenovirus adenoviral vaccines murine models |
| url | https://www.mdpi.com/1999-4915/14/3/601 |
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