Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli
The hepatitis A virus (HAV) HAF-203 strain was isolated from an acute case of HAV infection. The primary isolation of HAF-203 in Brazil and its adaptation to the FRhK-4 cell lineage allowed the production of large amounts of viral particles enabling molecular characterization of the first HAV isolat...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2006-11-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700009 |
| _version_ | 1797757212980936704 |
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| author | Marcia L Baptista Messias Silva Maria Amélia de Lima Clara FT Yoshida Ana Maria C Gaspar Ricardo Galler |
| author_facet | Marcia L Baptista Messias Silva Maria Amélia de Lima Clara FT Yoshida Ana Maria C Gaspar Ricardo Galler |
| author_sort | Marcia L Baptista |
| collection | DOAJ |
| description | The hepatitis A virus (HAV) HAF-203 strain was isolated from an acute case of HAV infection. The primary isolation of HAF-203 in Brazil and its adaptation to the FRhK-4 cell lineage allowed the production of large amounts of viral particles enabling molecular characterization of the first HAV isolate in Brazil. The aim of our study was to determine the nucleotide sequence of the HAF-203 strain genome, compare it to other HAV genomes and highlight its genetic variability. The complete nucleotide sequence of the HAF-203 strain (7472 nucleotides) was compared to those obtained earlier by others for other HAV isolates. These analyses revealed 19 HAF-specific nucleotide sequence differences with 10 amino acid substitutions. Most of the non-conservative changes were located at VP1, 2C, and 3D genes, but the 3B region was the most variable. The availability of HAF-203 complementary DNA was useful for the production of the recombinant VP1 protein, which is a major determinant of viral infectivity. This recombinant protein was shown by enzyme-linked immunoassay and blotting, to be immunogenic and resemble the native protein, therefore suggesting its value as a reagent for incorporation into diagnostic tests. |
| first_indexed | 2024-03-12T18:12:44Z |
| format | Article |
| id | doaj.art-d7fc715e9969443fbdb6dec540de2570 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:12:44Z |
| publishDate | 2006-11-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-d7fc715e9969443fbdb6dec540de25702023-08-02T09:14:55ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602006-11-01101775976610.1590/S0074-02762006000700009Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coliMarcia L BaptistaMessias SilvaMaria Amélia de LimaClara FT YoshidaAna Maria C GasparRicardo GallerThe hepatitis A virus (HAV) HAF-203 strain was isolated from an acute case of HAV infection. The primary isolation of HAF-203 in Brazil and its adaptation to the FRhK-4 cell lineage allowed the production of large amounts of viral particles enabling molecular characterization of the first HAV isolate in Brazil. The aim of our study was to determine the nucleotide sequence of the HAF-203 strain genome, compare it to other HAV genomes and highlight its genetic variability. The complete nucleotide sequence of the HAF-203 strain (7472 nucleotides) was compared to those obtained earlier by others for other HAV isolates. These analyses revealed 19 HAF-specific nucleotide sequence differences with 10 amino acid substitutions. Most of the non-conservative changes were located at VP1, 2C, and 3D genes, but the 3B region was the most variable. The availability of HAF-203 complementary DNA was useful for the production of the recombinant VP1 protein, which is a major determinant of viral infectivity. This recombinant protein was shown by enzyme-linked immunoassay and blotting, to be immunogenic and resemble the native protein, therefore suggesting its value as a reagent for incorporation into diagnostic tests.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700009hepatitis A virus (HAV)HAF-203 straincomplete genomeVP1 gene recombinant proteinEscherichia coli expressionHAV diagnosis |
| spellingShingle | Marcia L Baptista Messias Silva Maria Amélia de Lima Clara FT Yoshida Ana Maria C Gaspar Ricardo Galler Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli Memorias do Instituto Oswaldo Cruz hepatitis A virus (HAV) HAF-203 strain complete genome VP1 gene recombinant protein Escherichia coli expression HAV diagnosis |
| title | Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli |
| title_full | Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli |
| title_fullStr | Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli |
| title_full_unstemmed | Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli |
| title_short | Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli |
| title_sort | genetic variability of hepatitis a virus strain haf 203 isolated in brazil and expression of the vp1 gene in escherichia coli |
| topic | hepatitis A virus (HAV) HAF-203 strain complete genome VP1 gene recombinant protein Escherichia coli expression HAV diagnosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700009 |
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