Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
Abstract The immunogenicity and protective efficacy of vaccines can be enhanced by the selective delivery of antigens to the antigen-presenting cells (APCs). In this study, H9N2 avian influenza virus haemagglutinin (HA) antigen, was targeted by fusing it to single-chain fragment variable (scFv) anti...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-07-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-021-00350-3 |
| _version_ | 1797641780473102336 |
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| author | Angita Shrestha Jean-Remy Sadeyen Deimante Lukosaityte Pengxiang Chang Adrian Smith Marielle Van Hulten Munir Iqbal |
| author_facet | Angita Shrestha Jean-Remy Sadeyen Deimante Lukosaityte Pengxiang Chang Adrian Smith Marielle Van Hulten Munir Iqbal |
| author_sort | Angita Shrestha |
| collection | DOAJ |
| description | Abstract The immunogenicity and protective efficacy of vaccines can be enhanced by the selective delivery of antigens to the antigen-presenting cells (APCs). In this study, H9N2 avian influenza virus haemagglutinin (HA) antigen, was targeted by fusing it to single-chain fragment variable (scFv) antibodies specific to CD83 receptor expressed on chicken APCs. We observed an increased level of IFNγ, IL6, IL1β, IL4, and CxCLi2 mRNA upon stimulation of chicken splenocytes ex vivo by CD83 scFv targeted H9HA. In addition, CD83 scFv targeted H9HA induced higher serum haemagglutinin inhibition activity and virus neutralising antibodies compared to untargeted H9HA, with induction of antibodies as early as day 6 post primary vaccination. Furthermore, chickens vaccinated with CD83 scFv targeted H9HA showed reduced H9N2 challenge virus shedding compared to untargeted H9HA. These results suggest that targeting antigens to CD83 receptors could improve the efficacy of poultry vaccines. |
| first_indexed | 2024-03-11T13:50:35Z |
| format | Article |
| id | doaj.art-d7ffdce6ab774f3594d034b069050909 |
| institution | Directory Open Access Journal |
| issn | 2059-0105 |
| language | English |
| last_indexed | 2024-03-11T13:50:35Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj.art-d7ffdce6ab774f3594d034b0690509092023-11-02T09:20:53ZengNature Portfolionpj Vaccines2059-01052021-07-016111210.1038/s41541-021-00350-3Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenzaAngita Shrestha0Jean-Remy Sadeyen1Deimante Lukosaityte2Pengxiang Chang3Adrian Smith4Marielle Van Hulten5Munir Iqbal6The Pirbright Institute, Pirbright, WokingThe Pirbright Institute, Pirbright, WokingThe Pirbright Institute, Pirbright, WokingThe Pirbright Institute, Pirbright, WokingDepartment of Zoology, Peter Medawar Building, University of OxfordGlobal Poultry R&D Biologicals Boxmeer, Intervet International BV, MSD Animal HealthThe Pirbright Institute, Pirbright, WokingAbstract The immunogenicity and protective efficacy of vaccines can be enhanced by the selective delivery of antigens to the antigen-presenting cells (APCs). In this study, H9N2 avian influenza virus haemagglutinin (HA) antigen, was targeted by fusing it to single-chain fragment variable (scFv) antibodies specific to CD83 receptor expressed on chicken APCs. We observed an increased level of IFNγ, IL6, IL1β, IL4, and CxCLi2 mRNA upon stimulation of chicken splenocytes ex vivo by CD83 scFv targeted H9HA. In addition, CD83 scFv targeted H9HA induced higher serum haemagglutinin inhibition activity and virus neutralising antibodies compared to untargeted H9HA, with induction of antibodies as early as day 6 post primary vaccination. Furthermore, chickens vaccinated with CD83 scFv targeted H9HA showed reduced H9N2 challenge virus shedding compared to untargeted H9HA. These results suggest that targeting antigens to CD83 receptors could improve the efficacy of poultry vaccines.https://doi.org/10.1038/s41541-021-00350-3 |
| spellingShingle | Angita Shrestha Jean-Remy Sadeyen Deimante Lukosaityte Pengxiang Chang Adrian Smith Marielle Van Hulten Munir Iqbal Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza npj Vaccines |
| title | Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza |
| title_full | Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza |
| title_fullStr | Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza |
| title_full_unstemmed | Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza |
| title_short | Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza |
| title_sort | selectively targeting haemagglutinin antigen to chicken cd83 receptor induces faster and stronger immunity against avian influenza |
| url | https://doi.org/10.1038/s41541-021-00350-3 |
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