Redox State of Human Serum Albumin in Multiple Sclerosis: A Pilot Study
Like in many other pathologies, oxidative stress is involved in the development of neurodegenerative disorders. Human serum albumin (HSA) is the main protein in different body fluids including cerebrospinal fluid (CSF). By its redox state in terms of cysteine-34, albumin serves as marker for oxidati...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/24/15806 |
| _version_ | 1797457293078429696 |
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| author | Margret Paar Katharina Seifried Gerhard Cvirn Arabella Buchmann Michael Khalil Karl Oettl |
| author_facet | Margret Paar Katharina Seifried Gerhard Cvirn Arabella Buchmann Michael Khalil Karl Oettl |
| author_sort | Margret Paar |
| collection | DOAJ |
| description | Like in many other pathologies, oxidative stress is involved in the development of neurodegenerative disorders. Human serum albumin (HSA) is the main protein in different body fluids including cerebrospinal fluid (CSF). By its redox state in terms of cysteine-34, albumin serves as marker for oxidative burden. We aimed to evaluate the redox state of HSA in patients with multiple sclerosis in serum and CSF in comparison to controls to identify possible correlations with disease activity and severity. Samples were stored at −70 °C until analysis by HPLC for the determination of albumin redox state in terms of the fractions of human mercaptalbumin (HMA), human nonmercaptalbumin1 (HNA1), and human nonmercaptalbumin2 (HNA2). Albumin in CSF showed significantly higher fractions of the reduced form HMA and decreased HNA1 and HNA2. There was no difference between albumin redox states in serum of patients and controls. In CSF of patients HNA2 showed a trend to higher fractions compared to controls. Albumin redox state in serum was associated with physical disability in remission while albumin redox state in CSF was related to disease activity. Thus, albumin redox state in serum and CSF of patients in relation to disease condition merits further investigation. |
| first_indexed | 2024-03-09T16:20:07Z |
| format | Article |
| id | doaj.art-d803444fcff8474eaf4612d1900cf1aa |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:20:07Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d803444fcff8474eaf4612d1900cf1aa2023-11-24T15:28:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123241580610.3390/ijms232415806Redox State of Human Serum Albumin in Multiple Sclerosis: A Pilot StudyMargret Paar0Katharina Seifried1Gerhard Cvirn2Arabella Buchmann3Michael Khalil4Karl Oettl5Division of Medicinal Chemistry, Otto Loewi Research Center, Medical University of Graz, Neue Stiftingtalstraße 6, 8010 Graz, AustriaDivision of Medicinal Chemistry, Otto Loewi Research Center, Medical University of Graz, Neue Stiftingtalstraße 6, 8010 Graz, AustriaDivision of Medicinal Chemistry, Otto Loewi Research Center, Medical University of Graz, Neue Stiftingtalstraße 6, 8010 Graz, AustriaDepartment of Neurology, Medical University of Graz, 8036 Graz, AustriaDepartment of Neurology, Medical University of Graz, 8036 Graz, AustriaDivision of Medicinal Chemistry, Otto Loewi Research Center, Medical University of Graz, Neue Stiftingtalstraße 6, 8010 Graz, AustriaLike in many other pathologies, oxidative stress is involved in the development of neurodegenerative disorders. Human serum albumin (HSA) is the main protein in different body fluids including cerebrospinal fluid (CSF). By its redox state in terms of cysteine-34, albumin serves as marker for oxidative burden. We aimed to evaluate the redox state of HSA in patients with multiple sclerosis in serum and CSF in comparison to controls to identify possible correlations with disease activity and severity. Samples were stored at −70 °C until analysis by HPLC for the determination of albumin redox state in terms of the fractions of human mercaptalbumin (HMA), human nonmercaptalbumin1 (HNA1), and human nonmercaptalbumin2 (HNA2). Albumin in CSF showed significantly higher fractions of the reduced form HMA and decreased HNA1 and HNA2. There was no difference between albumin redox states in serum of patients and controls. In CSF of patients HNA2 showed a trend to higher fractions compared to controls. Albumin redox state in serum was associated with physical disability in remission while albumin redox state in CSF was related to disease activity. Thus, albumin redox state in serum and CSF of patients in relation to disease condition merits further investigation.https://www.mdpi.com/1422-0067/23/24/15806human serum albuminhuman mercaptalbuminhuman nonmercaptalbuminhuman cerebrospinal fluid albuminalbumin redox statemultiple sclerosis |
| spellingShingle | Margret Paar Katharina Seifried Gerhard Cvirn Arabella Buchmann Michael Khalil Karl Oettl Redox State of Human Serum Albumin in Multiple Sclerosis: A Pilot Study International Journal of Molecular Sciences human serum albumin human mercaptalbumin human nonmercaptalbumin human cerebrospinal fluid albumin albumin redox state multiple sclerosis |
| title | Redox State of Human Serum Albumin in Multiple Sclerosis: A Pilot Study |
| title_full | Redox State of Human Serum Albumin in Multiple Sclerosis: A Pilot Study |
| title_fullStr | Redox State of Human Serum Albumin in Multiple Sclerosis: A Pilot Study |
| title_full_unstemmed | Redox State of Human Serum Albumin in Multiple Sclerosis: A Pilot Study |
| title_short | Redox State of Human Serum Albumin in Multiple Sclerosis: A Pilot Study |
| title_sort | redox state of human serum albumin in multiple sclerosis a pilot study |
| topic | human serum albumin human mercaptalbumin human nonmercaptalbumin human cerebrospinal fluid albumin albumin redox state multiple sclerosis |
| url | https://www.mdpi.com/1422-0067/23/24/15806 |
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