ROS generating BODIPY loaded nanoparticles for photodynamic eradication of biofilms
Bacterial biofilms can pose a serious health risk to humans and are less susceptible to antibiotics and disinfection than planktonic bacteria. Here, a novel method for biofilm eradication based on antimicrobial photodynamic therapy utilizing a nanoparticle in conjunction with a BODIPY derivative as...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1274715/full |
| _version_ | 1797660270989934592 |
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| author | Charlotte Kromer Charlotte Kromer Karin Schwibbert Sebastian Radunz Dorothea Thiele Peter Laux Andreas Luch Andreas Luch Harald R. Tschiche |
| author_facet | Charlotte Kromer Charlotte Kromer Karin Schwibbert Sebastian Radunz Dorothea Thiele Peter Laux Andreas Luch Andreas Luch Harald R. Tschiche |
| author_sort | Charlotte Kromer |
| collection | DOAJ |
| description | Bacterial biofilms can pose a serious health risk to humans and are less susceptible to antibiotics and disinfection than planktonic bacteria. Here, a novel method for biofilm eradication based on antimicrobial photodynamic therapy utilizing a nanoparticle in conjunction with a BODIPY derivative as photosensitizer was developed. Reactive oxygen species are generated upon illumination with visible light and lead to a strong, controllable and persistent eradication of both planktonic bacteria and biofilms. One of the biggest challenges in biofilm eradication is the penetration of the antimicrobial agent into the biofilm and its matrix. A biocompatible hydrophilic nanoparticle was utilized as a delivery system for the hydrophobic BODIPY dye and enabled its accumulation within the biofilm. This key feature of delivering the antimicrobial agent to the site of action where it is activated resulted in effective eradication of all tested biofilms. Here, 3 bacterial species that commonly form clinically relevant pathogenic biofilms were selected: Escherichia coli, Staphylococcus aureus and Streptococcus mutans. The development of this antimicrobial photodynamic therapy tool for biofilm eradication takes a promising step towards new methods for the much needed treatment of pathogenic biofilms. |
| first_indexed | 2024-03-11T18:28:21Z |
| format | Article |
| id | doaj.art-d803be63f0e845feb133a1ee2f5f1bb3 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T18:28:21Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d803be63f0e845feb133a1ee2f5f1bb32023-10-13T15:13:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-10-011410.3389/fmicb.2023.12747151274715ROS generating BODIPY loaded nanoparticles for photodynamic eradication of biofilmsCharlotte Kromer0Charlotte Kromer1Karin Schwibbert2Sebastian Radunz3Dorothea Thiele4Peter Laux5Andreas Luch6Andreas Luch7Harald R. Tschiche8Department Chemicals and Product Safety, Product Materials and Nanotechnology, German Federal Institute for Risk Assessment, Berlin, GermanyInstitute of Pharmacy, Freie Universität Berlin, Berlin, GermanyDepartment Materials and the Environment, Biodeterioration and Reference Organisms, Federal Institute for Materials Research and Testing, Berlin, GermanynanoPET Pharma GmbH, Berlin, GermanyDepartment Materials and the Environment, Biodeterioration and Reference Organisms, Federal Institute for Materials Research and Testing, Berlin, GermanyDepartment Chemicals and Product Safety, Product Materials and Nanotechnology, German Federal Institute for Risk Assessment, Berlin, GermanyDepartment Chemicals and Product Safety, Product Materials and Nanotechnology, German Federal Institute for Risk Assessment, Berlin, GermanyInstitute of Pharmacy, Freie Universität Berlin, Berlin, GermanyDepartment Chemicals and Product Safety, Product Materials and Nanotechnology, German Federal Institute for Risk Assessment, Berlin, GermanyBacterial biofilms can pose a serious health risk to humans and are less susceptible to antibiotics and disinfection than planktonic bacteria. Here, a novel method for biofilm eradication based on antimicrobial photodynamic therapy utilizing a nanoparticle in conjunction with a BODIPY derivative as photosensitizer was developed. Reactive oxygen species are generated upon illumination with visible light and lead to a strong, controllable and persistent eradication of both planktonic bacteria and biofilms. One of the biggest challenges in biofilm eradication is the penetration of the antimicrobial agent into the biofilm and its matrix. A biocompatible hydrophilic nanoparticle was utilized as a delivery system for the hydrophobic BODIPY dye and enabled its accumulation within the biofilm. This key feature of delivering the antimicrobial agent to the site of action where it is activated resulted in effective eradication of all tested biofilms. Here, 3 bacterial species that commonly form clinically relevant pathogenic biofilms were selected: Escherichia coli, Staphylococcus aureus and Streptococcus mutans. The development of this antimicrobial photodynamic therapy tool for biofilm eradication takes a promising step towards new methods for the much needed treatment of pathogenic biofilms.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1274715/fullbiofilmdisinfectionantimicrobialsphotodynamic therapyaPDTnanoparticles |
| spellingShingle | Charlotte Kromer Charlotte Kromer Karin Schwibbert Sebastian Radunz Dorothea Thiele Peter Laux Andreas Luch Andreas Luch Harald R. Tschiche ROS generating BODIPY loaded nanoparticles for photodynamic eradication of biofilms Frontiers in Microbiology biofilm disinfection antimicrobials photodynamic therapy aPDT nanoparticles |
| title | ROS generating BODIPY loaded nanoparticles for photodynamic eradication of biofilms |
| title_full | ROS generating BODIPY loaded nanoparticles for photodynamic eradication of biofilms |
| title_fullStr | ROS generating BODIPY loaded nanoparticles for photodynamic eradication of biofilms |
| title_full_unstemmed | ROS generating BODIPY loaded nanoparticles for photodynamic eradication of biofilms |
| title_short | ROS generating BODIPY loaded nanoparticles for photodynamic eradication of biofilms |
| title_sort | ros generating bodipy loaded nanoparticles for photodynamic eradication of biofilms |
| topic | biofilm disinfection antimicrobials photodynamic therapy aPDT nanoparticles |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1274715/full |
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