First report on metagenomics and their predictive functional analysis of fermented bamboo shoot food of Tripura, North East India
Moiya pansung, mileye amileye, moiya koshak, and midukeye are naturally fermented bamboo shoot foods of Tripura. The present study aimed to reveal the whole microbial community structure of naturally fermented moiya pangsung, mileye amileye, moiya koshak, and midukeye along with the prediction of mi...
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| Format: | Article |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1158411/full |
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| author | Rohit Das Buddhiman Tamang Ishfaq Nabi Najar Nagendra Thakur Krishnendu Mondal |
| author_facet | Rohit Das Buddhiman Tamang Ishfaq Nabi Najar Nagendra Thakur Krishnendu Mondal |
| author_sort | Rohit Das |
| collection | DOAJ |
| description | Moiya pansung, mileye amileye, moiya koshak, and midukeye are naturally fermented bamboo shoot foods of Tripura. The present study aimed to reveal the whole microbial community structure of naturally fermented moiya pangsung, mileye amileye, moiya koshak, and midukeye along with the prediction of microbial functional profiles by shotgun metagenomic sequence analysis. The metataxonomic profile of moiya pangsung, mileye amileye, moiya koshak, and midukeye samples showed different domains, viz., bacteria (97.70%) followed by the virus (0.76%), unclassified (0.09%), eukaryotes (1.46%) and archaea (0.05%). Overall, 49 phyla, 409 families, 841 genera, and 1,799 species were found in all the fermented bamboo shoot samples collected from different places of Tripura. Firmicutes was the most abundant phylum (89.28%) followed by Proteobacteria (5.13%), Bacteroidetes (4.38%), Actinobacteria (1.02%), and Fusobacteria (0.17%). Lactiplantibacillus plantarum was the most abundant species in moiya pangsung, mileye amileye, moiya koshak, and midukeye followed by Lactococcus lactis, Levilactobacillus brevis, Leuconostoc mesenteroides, Weissella paramesenteroides, Leuconostoc kimchii, Pediococcus pentosaceus, Leuconostoc gasicomitatum, and Lacticaseibacillus casei. A few phyla of fungus were found, viz., Ascomycota, Basidiomycota, and Glomeromycota, where Ascomycota was present in high abundance. Functional analysis of moiya pangsung, mileye amileye, moiya koshak, and midukeye metagenome revealed the genes for the synthesis and metabolism of a wide range of bioactive compounds including, various essential amino acids, and conjugated amino acids. The abundance profile and predictive analysis of fermented bamboo shoots revealed a huge plethora of essential microorganisms and KEGG analysis revealed genes for amino acid metabolism, pectin degradation, lipid metabolism, and many other essential pathways that can be essential for the improvement of nutritional and sensory qualities of the fermented bamboo shoot products. |
| first_indexed | 2024-04-09T18:24:51Z |
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| id | doaj.art-d8050877ab6a482f95ac249ef0ad00ee |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T18:24:51Z |
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| spelling | doaj.art-d8050877ab6a482f95ac249ef0ad00ee2023-04-12T05:12:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11584111158411First report on metagenomics and their predictive functional analysis of fermented bamboo shoot food of Tripura, North East IndiaRohit Das0Buddhiman Tamang1Ishfaq Nabi Najar2Nagendra Thakur3Krishnendu Mondal4Department of Microbiology, Sikkim University, Gangtok, IndiaDepartment of Microbiology, Sikkim University, Gangtok, IndiaDepartment of Microbiology, Sikkim University, Gangtok, IndiaDepartment of Microbiology, Sikkim University, Gangtok, IndiaDepartment of Microbiology, Vidyasagar University, Midnapore, IndiaMoiya pansung, mileye amileye, moiya koshak, and midukeye are naturally fermented bamboo shoot foods of Tripura. The present study aimed to reveal the whole microbial community structure of naturally fermented moiya pangsung, mileye amileye, moiya koshak, and midukeye along with the prediction of microbial functional profiles by shotgun metagenomic sequence analysis. The metataxonomic profile of moiya pangsung, mileye amileye, moiya koshak, and midukeye samples showed different domains, viz., bacteria (97.70%) followed by the virus (0.76%), unclassified (0.09%), eukaryotes (1.46%) and archaea (0.05%). Overall, 49 phyla, 409 families, 841 genera, and 1,799 species were found in all the fermented bamboo shoot samples collected from different places of Tripura. Firmicutes was the most abundant phylum (89.28%) followed by Proteobacteria (5.13%), Bacteroidetes (4.38%), Actinobacteria (1.02%), and Fusobacteria (0.17%). Lactiplantibacillus plantarum was the most abundant species in moiya pangsung, mileye amileye, moiya koshak, and midukeye followed by Lactococcus lactis, Levilactobacillus brevis, Leuconostoc mesenteroides, Weissella paramesenteroides, Leuconostoc kimchii, Pediococcus pentosaceus, Leuconostoc gasicomitatum, and Lacticaseibacillus casei. A few phyla of fungus were found, viz., Ascomycota, Basidiomycota, and Glomeromycota, where Ascomycota was present in high abundance. Functional analysis of moiya pangsung, mileye amileye, moiya koshak, and midukeye metagenome revealed the genes for the synthesis and metabolism of a wide range of bioactive compounds including, various essential amino acids, and conjugated amino acids. The abundance profile and predictive analysis of fermented bamboo shoots revealed a huge plethora of essential microorganisms and KEGG analysis revealed genes for amino acid metabolism, pectin degradation, lipid metabolism, and many other essential pathways that can be essential for the improvement of nutritional and sensory qualities of the fermented bamboo shoot products.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1158411/fullfermented bamboo shootmeloncana bacciferashotgun metagenomicsmicrobial community analysisfunctional gene analysis |
| spellingShingle | Rohit Das Buddhiman Tamang Ishfaq Nabi Najar Nagendra Thakur Krishnendu Mondal First report on metagenomics and their predictive functional analysis of fermented bamboo shoot food of Tripura, North East India Frontiers in Microbiology fermented bamboo shoot meloncana baccifera shotgun metagenomics microbial community analysis functional gene analysis |
| title | First report on metagenomics and their predictive functional analysis of fermented bamboo shoot food of Tripura, North East India |
| title_full | First report on metagenomics and their predictive functional analysis of fermented bamboo shoot food of Tripura, North East India |
| title_fullStr | First report on metagenomics and their predictive functional analysis of fermented bamboo shoot food of Tripura, North East India |
| title_full_unstemmed | First report on metagenomics and their predictive functional analysis of fermented bamboo shoot food of Tripura, North East India |
| title_short | First report on metagenomics and their predictive functional analysis of fermented bamboo shoot food of Tripura, North East India |
| title_sort | first report on metagenomics and their predictive functional analysis of fermented bamboo shoot food of tripura north east india |
| topic | fermented bamboo shoot meloncana baccifera shotgun metagenomics microbial community analysis functional gene analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1158411/full |
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