Hypoallergenic properties of donkey's milk: a preliminary study
Cow's milk protein allergy (CMPA) is an abnormal immunological response to cow milk proteins, which results in IgE-mediated reactions. The therapeutic strategy to respond to CMPA envisages the total elimination of milk or the administration of cow's milk substitutes. For this reason the us...
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2014-06-01
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| Series: | Veterinaria Italiana |
| Subjects: | |
| Online Access: | http://www.izs.it/vet_italiana/2014/50_2/VetIt_219_125_5.pdf |
| _version_ | 1819150853215354880 |
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| author | Silvia Vincenzetti Laura Foghini Stefania Pucciarelli Valeria Polzonetti Natalina Cammertoni Daniela Beghelli Paolo Polidori |
| author_facet | Silvia Vincenzetti Laura Foghini Stefania Pucciarelli Valeria Polzonetti Natalina Cammertoni Daniela Beghelli Paolo Polidori |
| author_sort | Silvia Vincenzetti |
| collection | DOAJ |
| description | Cow's milk protein allergy (CMPA) is an abnormal immunological response to cow milk proteins, which results in IgE-mediated reactions. The therapeutic strategy to respond to CMPA envisages the total elimination of milk or the administration of cow's milk substitutes. For this reason the use of milk from other mammalian species was tested. Among them, donkey's milk proved to be the best alternative in feeding infants affected by CMPA, since its chemical composition is comparable to human milk. In this work an in vitro study was performed in order to analyze the IgE reactivity to milk protein allergens from cow, donkey and goat. In particular, immunoblotting experiments using sera from milk-allergic and non-allergic adult volunteers were conducted with the aim of verifying the hypoallergenic property of donkey's milk. This study provided a preliminary evidence of the hypoallergenicity of donkey's milk when compared to bovine and goat milk. Considering the obtained results, it would be possible to develop a sensitive diagnostic method for CMPA detection, based on chromatographic and immunoblotting analysis. |
| first_indexed | 2024-12-22T14:24:06Z |
| format | Article |
| id | doaj.art-d8094b5b9cc14e3aae99d0e9de437be2 |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-12-22T14:24:06Z |
| publishDate | 2014-06-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-d8094b5b9cc14e3aae99d0e9de437be22022-12-21T18:22:55ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272014-06-015029910710.12834/VetIt.219.125.5Hypoallergenic properties of donkey's milk: a preliminary studySilvia Vincenzetti0Laura Foghini1Stefania Pucciarelli2Valeria Polzonetti3Natalina Cammertoni4Daniela Beghelli5Paolo Polidori6School of Biosciences and Veterinary Medicine, section of Veterinary Medicine, University of Camerino, via circonvallazione 93/95, 62024 Matelica (MC), Italy. Tel.: +39 0737 403462, Fax: +39 0737 403402, e-mail: silvia.vincenzetti@unicam.itSchool of Biosciences and Veterinary Medicine, section of Biosciences, Via Gentile III da Varano, 62032 Camerino (MC), ItalySchool of Biosciences and Veterinary Medicine, section of Biosciences, Via Gentile III da Varano, 62032 Camerino (MC), ItalySchool of Biosciences and Veterinary Medicine, section of Biosciences, Via Gentile III da Varano, 62032 Camerino (MC), ItalySchool of Biosciences and Veterinary Medicine, section of Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica (MC), ItalySchool of Biosciences and Veterinary Medicine, section of Biosciences, Via Gentile III da Varano, 62032 Camerino (MC), ItalySchool of Pharmacy, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica (MC), ItalyCow's milk protein allergy (CMPA) is an abnormal immunological response to cow milk proteins, which results in IgE-mediated reactions. The therapeutic strategy to respond to CMPA envisages the total elimination of milk or the administration of cow's milk substitutes. For this reason the use of milk from other mammalian species was tested. Among them, donkey's milk proved to be the best alternative in feeding infants affected by CMPA, since its chemical composition is comparable to human milk. In this work an in vitro study was performed in order to analyze the IgE reactivity to milk protein allergens from cow, donkey and goat. In particular, immunoblotting experiments using sera from milk-allergic and non-allergic adult volunteers were conducted with the aim of verifying the hypoallergenic property of donkey's milk. This study provided a preliminary evidence of the hypoallergenicity of donkey's milk when compared to bovine and goat milk. Considering the obtained results, it would be possible to develop a sensitive diagnostic method for CMPA detection, based on chromatographic and immunoblotting analysis.http://www.izs.it/vet_italiana/2014/50_2/VetIt_219_125_5.pdfCationic exchange chromatographyCMPACow's milkDonkey's milkGoat's milkImmunoblottingHypoallergenicityReversed-phase chromatography |
| spellingShingle | Silvia Vincenzetti Laura Foghini Stefania Pucciarelli Valeria Polzonetti Natalina Cammertoni Daniela Beghelli Paolo Polidori Hypoallergenic properties of donkey's milk: a preliminary study Veterinaria Italiana Cationic exchange chromatography CMPA Cow's milk Donkey's milk Goat's milk Immunoblotting Hypoallergenicity Reversed-phase chromatography |
| title | Hypoallergenic properties of donkey's milk: a preliminary study |
| title_full | Hypoallergenic properties of donkey's milk: a preliminary study |
| title_fullStr | Hypoallergenic properties of donkey's milk: a preliminary study |
| title_full_unstemmed | Hypoallergenic properties of donkey's milk: a preliminary study |
| title_short | Hypoallergenic properties of donkey's milk: a preliminary study |
| title_sort | hypoallergenic properties of donkey s milk a preliminary study |
| topic | Cationic exchange chromatography CMPA Cow's milk Donkey's milk Goat's milk Immunoblotting Hypoallergenicity Reversed-phase chromatography |
| url | http://www.izs.it/vet_italiana/2014/50_2/VetIt_219_125_5.pdf |
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